APC/Fire™ 750 anti-mouse CD138 (Syndecan-1) Antibody, CD138, 281-2

Pricing & Availability

Clone: 281-2 (See other available formats)
Regulatory Status: RUO
Other Names: Syndecan-1
Isotype: Rat IgG2a, κ
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Product Citations: publications

281-2_APCFire750_CD138_Antibody_030822 — C577BL/6 mouse bone marrow cells were stained with anti-mouse CD19 PE and anti-mouse CD138 (clone 281-2) APC/Fire™ 750 (Left) or rat IgG2a, κ APC/Fire™ 750 isotype control (right).

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Size

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142541

25 µg

141€

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142542

100 µg

306€

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Description

CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant plasma cells, pre-B cells, mesenchymal cells, epithelial cells, and endothelial cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse mammary gland epithelial cell line NMuMG

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Preparation

The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.

Concentration

0.2 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue³ and formalin-fixed paraffin embedded tissue⁴and immunofluorescent staining^2,3.

Application References

Jalkanen M, et al. 1985. J. Cell. Biol. 101:976. (FC)
Miettinen H, et al. 1994. J. Cell. Sci. 107:1571. (IF)
Li Q, et al. 2002. Cell 111:635. (IF, IHC)
McCarthy BA, et al. 2012. BMC Cancer. 12:203. (IHC)

RRID

AB_2924473 (BioLegend Cat. No. 142541)
AB_2924473 (BioLegend Cat. No. 142542)

Antigen Details

Structure: Transmembrane heparan sulfate proteoglycan; member of the syndecan proteoglycan family, 100-200 kD
Function: Cell adhesion, cell motility, cell migration, cell proliferation
Cell Type: B cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Zong F, et al. 2011. PLoS ONE 6:e14816.
2. Yamashita Y, et al. 1999. J. Immunol. 162:5940.
3. Sanderson RD, et al. 1989. Cell. Regul. 1:27.
Gene ID: 20969 View all products for this Gene ID
UniProt: View information about CD138 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Pathways

B cell Development and Activation

Related FAQs

Other Formats

View All CD138 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse CD138 (Syndecan-1)	281-2	FC,ICC,IHC-P,IHC-F
PE anti-mouse CD138 (Syndecan-1)	281-2	FC
APC anti-mouse CD138 (Syndecan-1)	281-2	FC
Brilliant Violet 421™ anti-mouse CD138 (Syndecan-1)	281-2	FC
PerCP/Cyanine5.5 anti-mouse CD138 (Syndecan-1)	281-2	FC
Biotin anti-mouse CD138 (Syndecan-1)	281-2	FC
PE/Cyanine7 anti-mouse CD138 (Syndecan-1)	281-2	FC
Brilliant Violet 605™ anti-mouse CD138 (Syndecan-1)	281-2	FC
Brilliant Violet 650™ anti-mouse CD138 (Syndecan-1)	281-2	FC
Brilliant Violet 711™ anti-mouse CD138 (Syndecan-1)	281-2	FC
Brilliant Violet 510™ anti-mouse CD138 (Syndecan-1)	281-2	FC
Alexa Fluor® 647 anti-mouse CD138 (Syndecan-1)	281-2	FC
PE/Dazzle™ 594 anti-mouse CD138 (Syndecan-1)	281-2	FC
APC/Cyanine7 anti-mouse CD138 (Syndecan-1)	281-2	FC
TotalSeq™-A0810 anti-mouse CD138 (Syndecan-1)	281-2	PG
Brilliant Violet 785™ anti-mouse CD138 (Syndecan-1)	281-2	FC
TotalSeq™-C0810 anti-mouse CD138 (Syndecan-1)	281-2	PG
TotalSeq™-B0810 anti-mouse CD138 (Syndecan-1)	281-2	PG
PE/Cyanine5 anti-mouse CD138 (Syndecan-1)	281-2	FC
APC/Fire™ 750 anti-mouse CD138 (Syndecan-1)	281-2	FC
PE/Fire™ 640 anti-mouse CD138 (Syndecan-1)	281-2	FC
PE/Fire™ 810 anti-mouse CD138 (Syndecan-1)	281-2	FC
PE/Fire™ 700 anti-mouse CD138 (Syndecan-1)	281-2	FC
Spark Red™ 718 anti-mouse CD138 (Syndecan-1) (Flexi-Fluor™)	281-2	FC
Spark Blue™ 574 anti-mouse CD138 (Syndecan-1) (Flexi-Fluor™)	281-2	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 03.08.2022

For Research Use Only. Not for diagnostic or therapeutic use.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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