PE/Fire™ 810 anti-mouse CD138 (Syndecan-1) Antibody, CD138, 281-2

Pricing & Availability

Clone: 281-2 (See other available formats)
Regulatory Status: RUO
Other Names: Syndecan-1
Isotype: Rat IgG2a, κ
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Product Citations: publications

281-2_PE_Fire810_CD138_Antibody_122022 — C57BL/6 mouse bone marrow cells were stained with anti-mouse CD19 FITC and anti-mouse CD138 (Syndecan-1) (clone 281-2) PE/Fire™ 810 (left) or stained with anti-mouse CD19 FITC only (right). Data shown is from gated on lymphoid cell population.

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142545

25 µg

141€

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Description

CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant plasma cells, pre-B cells, mesenchymal cells, epithelial cells, and endothelial cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse mammary gland epithelial cell line NMuMG
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 810 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Fire™ 810 has a maximum excitation of 488/561 nm and a maximum emission of 810 nm.

Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect PE/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue³ and formalin-fixed paraffin embedded tissue⁴and immunofluorescent staining^2,3.
RRID: AB_2936540 (BioLegend Cat. No. 142545)

Antigen Details

Structure: Transmembrane heparan sulfate proteoglycan; member of the syndecan proteoglycan family, 100-200 kD
Function: Cell adhesion, cell motility, cell migration, cell proliferation
Cell Type: B cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Zong F, et al. 2011. PLoS ONE 6:e14816.
2. Yamashita Y, et al. 1999. J. Immunol. 162:5940.
3. Sanderson RD, et al. 1989. Cell. Regul. 1:27.
Gene ID: 20969 View all products for this Gene ID
UniProt: View information about CD138 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Pathways

B cell Development and Activation

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Other Formats

View All CD138 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse CD138 (Syndecan-1)	281-2	FC,ICC,IHC-P,IHC-F
PE anti-mouse CD138 (Syndecan-1)	281-2	FC
APC anti-mouse CD138 (Syndecan-1)	281-2	FC
Brilliant Violet 421™ anti-mouse CD138 (Syndecan-1)	281-2	FC
PerCP/Cyanine5.5 anti-mouse CD138 (Syndecan-1)	281-2	FC
Biotin anti-mouse CD138 (Syndecan-1)	281-2	FC
PE/Cyanine7 anti-mouse CD138 (Syndecan-1)	281-2	FC
Brilliant Violet 605™ anti-mouse CD138 (Syndecan-1)	281-2	FC
Brilliant Violet 650™ anti-mouse CD138 (Syndecan-1)	281-2	FC
Brilliant Violet 711™ anti-mouse CD138 (Syndecan-1)	281-2	FC
Brilliant Violet 510™ anti-mouse CD138 (Syndecan-1)	281-2	FC
Alexa Fluor® 647 anti-mouse CD138 (Syndecan-1)	281-2	FC
PE/Dazzle™ 594 anti-mouse CD138 (Syndecan-1)	281-2	FC
APC/Cyanine7 anti-mouse CD138 (Syndecan-1)	281-2	FC
TotalSeq™-A0810 anti-mouse CD138 (Syndecan-1)	281-2	PG
Brilliant Violet 785™ anti-mouse CD138 (Syndecan-1)	281-2	FC
TotalSeq™-C0810 anti-mouse CD138 (Syndecan-1)	281-2	PG
TotalSeq™-B0810 anti-mouse CD138 (Syndecan-1)	281-2	PG
PE/Cyanine5 anti-mouse CD138 (Syndecan-1)	281-2	FC
APC/Fire™ 750 anti-mouse CD138 (Syndecan-1)	281-2	FC
PE/Fire™ 640 anti-mouse CD138 (Syndecan-1)	281-2	FC
PE/Fire™ 810 anti-mouse CD138 (Syndecan-1)	281-2	FC
PE/Fire™ 700 anti-mouse CD138 (Syndecan-1)	281-2	FC
Spark Red™ 718 anti-mouse CD138 (Syndecan-1) (Flexi-Fluor™)	281-2	FC
Spark Blue™ 574 anti-mouse CD138 (Syndecan-1) (Flexi-Fluor™)	281-2	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 05.03.2023

For Research Use Only. Not for diagnostic or therapeutic use.

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