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Brilliant Violet 650™ anti-mouse CD138 (Syndecan-1) Antibody

Pricing & Availability
Clone
281-2 (See other available formats)
Regulatory Status
RUO
Other Names
Syndecan-1
Isotype
Rat IgG2a, κ
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Product Citations
publications
281-2_BV650_CD138_Antibody_FC_1_081413
C57BL/6 mouse bone marrow cells were stained with CD19 FITC and CD138 (clone 281-2) Brilliant Violet 650™ (top) or rat IgG2a, κ Brilliant Violet 650™ isotype control (bottom). Data shown is from gated on lymphoid cell population.
  • 281-2_BV650_CD138_Antibody_FC_1_081413
    C57BL/6 mouse bone marrow cells were stained with CD19 FITC and CD138 (clone 281-2) Brilliant Violet 650™ (top) or rat IgG2a, κ Brilliant Violet 650™ isotype control (bottom). Data shown is from gated on lymphoid cell population.
  • 281-2_BV650_CD138_Antibody_FC_2_081413
Compare all formats See Brilliant Violet 650™ spectral data
Cat # Size Price Quantity Check Availability Save
142517 125 µL 168€
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142518 50 µg 210€
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Description

CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant plasma cells, pre-B cells, mesenchymal cells, epithelial cells, and endothelial cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse mammary gland epithelial cell line NMuMG
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.4 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue3 and formalin-fixed paraffin embedded tissue4 and immunofluorescent staining2,3.

Application References
  1. Jalkanen M, et al. 1985. J. Cell. Biol. 101:976. (FC)
  2. Miettinen H, et al. 1994. J. Cell. Sci. 107:1571. (IF)
  3. Li Q, et al. 2002. Cell 111:635. (IF, IHC)
  4. McCarthy BA, et al. 2012. BMC Cancer. 12:203. (IHC)
Product Citations
  1. Lee JH, et al. 2022. Cell Rep. 38:110485. PubMed
  2. Schiepers A, et al. 2023. Nature. 615:482. PubMed
  3. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  4. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  5. Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed
  6. Mesin L, et al. 2020. Cell. 180(1):92-106.e11.. PubMed
RRID
AB_2650927 (BioLegend Cat. No. 142517)
AB_2650927 (BioLegend Cat. No. 142518)

Antigen Details

Structure
Transmembrane heparan sulfate proteoglycan; member of the syndecan proteoglycan family, 100-200 kD
Function
Cell adhesion, cell motility, cell migration, cell proliferation
Cell Type
B cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zong F, et al. 2011. PLoS ONE 6:e14816.
2. Yamashita Y, et al. 1999. J. Immunol. 162:5940.
3. Sanderson RD, et al. 1989. Cell. Regul. 1:27.

Gene ID
20969 View all products for this Gene ID
UniProt
View information about CD138 on UniProt.org

Other Formats

View All CD138 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD138 (Syndecan-1) 281-2 FC,ICC,IHC-P,IHC-F
PE anti-mouse CD138 (Syndecan-1) 281-2 FC
APC anti-mouse CD138 (Syndecan-1) 281-2 FC
Brilliant Violet 421™ anti-mouse CD138 (Syndecan-1) 281-2 FC
PerCP/Cyanine5.5 anti-mouse CD138 (Syndecan-1) 281-2 FC
Biotin anti-mouse CD138 (Syndecan-1) 281-2 FC
PE/Cyanine7 anti-mouse CD138 (Syndecan-1) 281-2 FC
Brilliant Violet 605™ anti-mouse CD138 (Syndecan-1) 281-2 FC
Brilliant Violet 650™ anti-mouse CD138 (Syndecan-1) 281-2 FC
Brilliant Violet 711™ anti-mouse CD138 (Syndecan-1) 281-2 FC
Brilliant Violet 510™ anti-mouse CD138 (Syndecan-1) 281-2 FC
Alexa Fluor® 647 anti-mouse CD138 (Syndecan-1) 281-2 FC
PE/Dazzle™ 594 anti-mouse CD138 (Syndecan-1) 281-2 FC
APC/Cyanine7 anti-mouse CD138 (Syndecan-1) 281-2 FC
TotalSeq™-A0810 anti-mouse CD138 (Syndecan-1) 281-2 PG
Brilliant Violet 785™ anti-mouse CD138 (Syndecan-1) 281-2 FC
TotalSeq™-C0810 anti-mouse CD138 (Syndecan-1) 281-2 PG
TotalSeq™-B0810 anti-mouse CD138 (Syndecan-1) 281-2 PG
PE/Cyanine5 anti-mouse CD138 (Syndecan-1) 281-2 FC
APC/Fire™ 750 anti-mouse CD138 (Syndecan-1) 281-2 FC
PE/Fire™ 640 anti-mouse CD138 (Syndecan-1) 281-2 FC
PE/Fire™ 810 anti-mouse CD138 (Syndecan-1) 281-2 FC
PE/Fire™ 700 anti-mouse CD138 (Syndecan-1) 281-2 FC
Spark Red™ 718 anti-mouse CD138 (Syndecan-1) (Flexi-Fluor™) 281-2 FC
Spark Blue™ 574 anti-mouse CD138 (Syndecan-1) (Flexi-Fluor™) 281-2 FC
Go To Top Version: 1    Revision Date: 08.15.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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