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Direct-Blot™ HRP anti-HA.11 Epitope Tag Antibody

Pricing & Availability
Clone
16B12 (See other available formats)
Regulatory Status
RUO
Other Names
HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
Isotype
Mouse IgG1, κ
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Product Citations
publications
16B12_DB-HRP_HA.11_Antibody_WB_042017
Total cell lysate from CHO (lane 1) and CHO stably transfected with HA tag fused protein (lane 2) (15 µg/lane) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:10,000 purified anti-HA.11 or 1:10,000 Direct-Blot™ HRP anti-HA.11 Antibody. For the purified anti-HA.11 antibody, proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading controls (lower). Lane M is the MW ladder.
  • 16B12_DB-HRP_HA.11_Antibody_WB_042017
    Total cell lysate from CHO (lane 1) and CHO stably transfected with HA tag fused protein (lane 2) (15 µg/lane) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:10,000 purified anti-HA.11 or 1:10,000 Direct-Blot™ HRP anti-HA.11 Antibody. For the purified anti-HA.11 antibody, proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading controls (lower). Lane M is the MW ladder.
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901520 25 µL 101 CHF
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901519 100 µL 340 CHF
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Description

The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106.  It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Monoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:10000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

Additional tested and reported applications of the 16B12 clone for the relevant formats include: western blot (WB), immunocytochemistry (ICC), immunoprecipitation (IP), and flow cytometry (FC).

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. The HA.11 antibody recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of the tagged protein. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.

Application References

(PubMed link indicates BioLegend citation)
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  2. Helliwell SB, et al. 2001. J Cell Biol. 153:649. (WB)
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  4. Royer Y, et al. 2005. J. Biol. Chem. 29:27251. (FC)
  5. Smith BA, et al. 2012. Genes Cancer. 3:550. (IHC) PubMed
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  63. Tam K, et al. 2016. MBio. 7:e02024-16. PubMed
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Product Citations
  1. Mansouri S, et al. 2022. J Immunol. 209:2114. PubMed
  2. Choi YJ, et al. 2023. Nat Commun. 14:2045. PubMed
  3. Sondo E et al. 2018. Cell chemical biology. 25(7):891-905 . PubMed
  4. Portales-Castillo I, et al. 2022. JBMR Plus. 6:e10604. PubMed
  5. Liu Z, et al. 2021. PLoS Pathog. 17:e1010123. PubMed
RRID
AB_2686981 (BioLegend Cat. No. 901520)
AB_2686981 (BioLegend Cat. No. 901519)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
Specificity (DOES NOT SHOW ON TDS):
HA.11
Specificity Alt (DOES NOT SHOW ON TDS):
HA.11
App Abbreviation (DOES NOT SHOW ON TDS):
WB
UniProt
View information about HA.11 on UniProt.org

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Go To Top Version: 1    Revision Date: 04.21.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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