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Alexa Fluor® 555 Donkey anti-rabbit IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4064 (See other available formats)
Regulatory Status
RUO
Isotype
Donkey Polyclonal Ig
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Product Citations
publications
Poly4064_AF555_IgG_Antibody_IF_030515
HeLa cells were fixed with 1% paraformaldehyde for ten minutes followed by permeabilization for ten minutes with 0.5% Triton X-100, and then blocked with 5% FBS for 30 minutes. 2.5 µg/ml of purified BRCA-1 (Poly26599) was added to the cells and left at 4°C overnight. The cells were then stained with 2.5 µg/ml of Alexa Fluor® 555 (Yellow) for 2 hours at room temperature. Nuclei were counterstained with DAPI and are shown in blue and phalloidin shown in red. The image was captured with a 40X objective.
  • Poly4064_AF555_IgG_Antibody_IF_030515
    HeLa cells were fixed with 1% paraformaldehyde for ten minutes followed by permeabilization for ten minutes with 0.5% Triton X-100, and then blocked with 5% FBS for 30 minutes. 2.5 µg/ml of purified BRCA-1 (Poly26599) was added to the cells and left at 4°C overnight. The cells were then stained with 2.5 µg/ml of Alexa Fluor® 555 (Yellow) for 2 hours at room temperature. Nuclei were counterstained with DAPI and are shown in blue and phalloidin shown in red. The image was captured with a 40X objective.
Compare all formats See Alexa Fluor® 555 spectral data
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406412 100 µg 141 CHF
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Description

This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rabbit
Antibody Type
Polyclonal
Host Species
Donkey
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 555 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 555 has an excitation maximum of 555 nm, and a maximum emission of 565 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.

Product Citations
  1. Neufert C, et al. 2020. Nature Protocols. 16(1):61-85. PubMed
  2. Tayoun T, et al. 2022. JCI Insight. 7: . PubMed
  3. Frigon ÉM, et al. 2022. Front Neuroanat. 16:957358. PubMed
  4. Chae SA, et al. 2023. J Physiol. 601:2371. PubMed
  5. Koop K, et al. 2023. Front Immunol. 14:1163198. PubMed
  6. Brown CW, et al. 2020. Developmental Cell. 51(5):575-586.e4.. PubMed
  7. Son JS, et al. 2020. Cell Rep. 33:108461. PubMed
  8. Biering SB et al. 2017. Cell host & microbe. 22(1):74-85 . PubMed
  9. Divito SJ, et al. 2020. J Clin Invest. 130:4624. PubMed
  10. Kissane RWP, et al. 2021. Journal of Anatomy. . PubMed
  11. Baba T, et al. 2020. eLife. 9:e54257.. PubMed
RRID
AB_2563181 (BioLegend Cat. No. 406412)
Go To Top Version: 3    Revision Date: 10.28.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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