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FITC Donkey anti-rabbit IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4064 (See other available formats)
Regulatory Status
RUO
Isotype
Donkey Polyclonal Ig
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Product Citations
publications
Poly4064_FITC_IgG_Antibody_IF_032613
Hela cells were stained with anti-GRASP65 (Poly6210) and secondarily labeled with FITC donkey anti-rabbit IgG (clone Poly4064).
  • Poly4064_FITC_IgG_Antibody_IF_032613
    Hela cells were stained with anti-GRASP65 (Poly6210) and secondarily labeled with FITC donkey anti-rabbit IgG (clone Poly4064).
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406403 100 µg 83 CHF
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Description

This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rabbit
Antibody Type
Polyclonal
Host Species
Donkey
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
FC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 2.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.

Product Citations
  1. González-Casimiro CM, et al. 2022. Int J Mol Sci. 23:. PubMed
  2. Shao W, et al. 2023. Biomedicines. 11:. PubMed
  3. Amirova KM, et al. 2023. Int J Mol Sci. 24:. PubMed
  4. Nicholas D, et al. 2015. Mol Cell Proteomics. 14:15. PubMed
  5. Fisher E, et al. 2021. Front Immunol. 11:602254. PubMed
  6. Jarosch S, et al. 2022. STAR Protoc. 3:101374. PubMed
  7. Zhao Y, et al. 2021. Front Immunol. 12:665442. PubMed
  8. Wong C, et al. 2014. J Leukoc Biol. 95:631. PubMed
  9. Sun Z, et al. 2021. eLife. 10:00. PubMed
  10. Callahan SM, et al. 2021. Curr Protoc. 1:e294. PubMed
  11. Tang H, et al. 2017. J Immunol. 199:570. PubMed
  12. Callahan S, et al. 2020. Cell Microbiol. 22:e13210. PubMed
  13. Woo YD, et al. 2020. J Allergy Clin Immunol. . PubMed
  14. Jarosch S, et al. 2021. Cell Rep Methods. 1:100104. PubMed
  15. Yang J, et al. 2020. Nature. 586:572. PubMed
  16. Kishore M et al. 2017. Immunity. 47(5):875-889 . PubMed
  17. Cai Y, et al. 2019. Cell Rep. 27:3034. PubMed
  18. Shen C, et al. 2021. Front Immunol. 12:680055. PubMed
  19. Niemeyer BF, et al. 2021. Nano Sel. . PubMed
  20. Yang Y, et al. 2020. J Clin Invest. 130:5508. PubMed
  21. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  22. Janczy J, et al. 2014. J Immunol. 193:5190. PubMed
  23. Daneshmandi S, et al. 2021. Cell Reports. 34(10):108831. PubMed
  24. Zeng G, et al. 2011. J Immunol. 187:190. PubMed
  25. Mohamadi F, et al. 2019. Biomed Rep. 10:183. PubMed
  26. Tittarelli A, et al. 2014. J Immunol. 192:1313. PubMed
  27. Ramos-Ramírez P, et al. 2021. Front Immunol. 12:677550. PubMed
  28. Che N, et al. 2021. Front Immunol. 12:626310. PubMed
  29. Ryzhov S, et al. 2012. J Pharmacol Exp Ther. 341:764. PubMed
  30. Liu F, et al. 2021. Front Microbiol. 12:692279. PubMed
RRID
AB_893531 (BioLegend Cat. No. 406403)

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Go To Top Version: 5    Revision Date: 03.16.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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