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Alexa Fluor® 594 anti-human CD15 (SSEA-1) Antibody

Pricing & Availability
Clone
HI98 (See other available formats)
Regulatory Status
RUO
Workshop
IV M141
Other Names
Lewis X, 3-FAL, 3-FL, LNFP III, Lex , SSEA-1, X-hapten
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
HI98_AF594_CD15_Antibody_IHCP_111716
Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was performed using BioLegend's Tris-Buffered Saline 1X (1.0M, pH7.4) at 95°C for 40 minutes. The tissue was washed with PBS/ 0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Afterwards, the tissue was stained with 5 µg/mL of anti-human CD8a (clone C8/144B) Alexa Fluor® 647 (green) and anti-human CD15 (clone HI98) Alexa Fluor® 594 (red) at 4°C overnight. The nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
  • HI98_AF594_CD15_Antibody_IHCP_111716
    Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was performed using BioLegend's Tris-Buffered Saline 1X (1.0M, pH7.4) at 95°C for 40 minutes. The tissue was washed with PBS/ 0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Afterwards, the tissue was stained with 5 µg/mL of anti-human CD8a (clone C8/144B) Alexa Fluor® 647 (green) and anti-human CD15 (clone HI98) Alexa Fluor® 594 (red) at 4°C overnight. The nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
Compare all formats See Alexa Fluor® 594 spectral data
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301917 100 µg 370 CHF
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Description

CD15 is 3-fucosyl-N-acetyllactosamine (3-FAL), also known as Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is expressed on granulocytes and monocytes. It has also been shown to be expressed on Langerhans cells and some malignant cells. CD15 has been implicated in adhesion, as well as chemotaxis, phagocytosis, and bactericidal activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Clone HI98 has been described to specifically react with 3-fucosyl-N-acetyllactosamine (3-FAL), also called X-hapten, SSEA-1, CD15, or Lewis X. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections5 and formalin-fixed paraffin-embedded tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  3. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  4. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  5. van Baarsen LG, et al. 2014. Arthritis Res. Ther. 16:426. (IHC)
RRID
AB_2632827 (BioLegend Cat. No. 301917)

Antigen Details

Structure
Poly-N-acetyllactosamine
Distribution

Neutrophils, eosinophils, monocytes

Function
Adhesion
Cell Type
Embryonic Stem Cells, Eosinophils, Monocytes, Neural Stem Cells, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Stocks SC, et al. 1990. Biochem. J. 268:275.

Gene ID
2526 View all products for this Gene ID
UniProt
View information about CD15 on UniProt.org
Go To Top Version: 1    Revision Date: 11.22.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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