Alexa Fluor 647 anti-Nanog Antibody anti-Nanog

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Clone: 16H3A48 (See other available formats)
Regulatory Status: RUO
Other Names: Homeobox Transcription Factor Nanog, Nanog Homeobox, Homeobox Protein NANOG, Homeobox Transcription Factor Nanog-Delta 48
Isotype: Mouse IgG1, κ
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16H3A48_A647_Nanog_Antibody_ICFC_011017 — NTERA-2 cells were treated with the True-Nuclear™ Transcription Factor Buffer Set and then intracellularly stained with anti-Nanog (clone 16H3A48) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ Alexa Fluor® 647 isotype control (open histogram).

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674210

100 µg

382 CHF

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Description

Nanog is a homeodomain-containing transcription factor that is essential for early embryonic development. It is primarily expressed in pluripotent embryonic stem (ES) cells and cancer cell lines, maintaining the undifferentiated status of the cells and is downregulated upon differentiation. Si-RNA mediated knockdown of Nanog in ES cells induces lineage differentiation. Overexpression of Nanog allows for the leukemia inhibitory factor (LIF)-independent self-renewal in undifferentiated ES cells. Nanog functionally works with OCT4 and SOX2 to support pluripotency and self-renewal in ES cells and the expression of Nanog is regulated by OCT4/SOX2 heterodimers. Expression of Nanog has been detected in several types of tumor cells. The expression level of Nanog in cancer cells has been shown to be correlated with the malignancy of cancers.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length NANOG recombinant protein expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
Application Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
RRID: AB_2650619 (BioLegend Cat. No. 674210)

Antigen Details

Structure: 305 amino acids with a predicted molecular weight of approximately 35 kD and contains a homeobox domain that is responsible for DNA binding.
Interaction: Interacts with ZFP281, SMAD1, and SALL4.
Cell Type: Embryonic Stem Cells
Biology Area: Cell Biology, Immunology, Stem Cells, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Yin D, et al. 2012. Int. J. Mol. Med. 29:587.
2. Villasante A, et al. 2011. Cell Cycle 10:1488.
3. Theunissen TW, et al. 2011. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 366:2222.
4. Pan G, et al. 2007. Cell Res. 17:42.
5. Kashyap V, et al. 2009. Stem Cells Dev. 18:1093.
6. Silva J, et al. 2009. Cell 138:722.
Gene ID: 79923 View all products for this Gene ID
UniProt: View information about Nanog on UniProt.org

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Other Formats

View All Nanog Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Nanog	16H3A48	WB,ICFC,ICC
Alexa Fluor® 594 anti-Nanog	16H3A48	ICC,IHC-P
Brilliant Violet 421™ anti-Nanog	16H3A48	ICC
Alexa Fluor® 488 anti-Nanog	16H3A48	ICC
Alexa Fluor® 647 anti-Nanog	16H3A48	ICFC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 01.10.2017

For Research Use Only. Not for diagnostic or therapeutic use.

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