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Purified anti-Nanog Antibody

Pricing & Availability
Clone
16H3A48 (See other available formats)
Regulatory Status
RUO
Other Names
Homeobox Transcription Factor Nanog, Nanog Homeobox, Homeobox Protein NANOG, Homeobox Transcription Factor Nanog-Delta 48
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_16H3A48_Purified_Nanog_Antibody_WB_121217
Total lysates (15 µg protein) from NTERA-2 (Positive control) and HeLa (Low expression negative control) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/ml) purified anti-Nanog antibody, clone 16H3A48. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • 1_16H3A48_Purified_Nanog_Antibody_WB_121217
    Total lysates (15 µg protein) from NTERA-2 (Positive control) and HeLa (Low expression negative control) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/ml) purified anti-Nanog antibody, clone 16H3A48. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • 2_16H3A48_Purified_Nanog_Antibody_ICFC_062019
    Human malignant pluripotent embryonal carcinoma cell line, NTERA‐2, was treated with the Nuclear Factor Fixation and Permeabilization Buffer Set, intracellularly stained with purified anti‐human Nanog (clone 16H3A48, filled histogram) or purified mouse IgG1, κ isotype control (open histogram), and then was followed by anti‐mouse IgG PE.
  • 3_16H3A48_Purified_Nanog_Antibody_ICC_062019
    Ntera-2 cells (left) and HeLa cells (right) were stained with purified anti-NANOG (clone 16H3A48) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green) antibody and Alexa Fluor 594™ conjugated phalloidin (red).
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674201 25 µg 147 CHF
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674202 100 µg 311 CHF
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Description

Nanog is a homeodomain-containing transcription factor that is essential for early embryonic development. It is primarily expressed in pluripotent embryonic stem (ES) cells and cancer cell lines, maintaining the undifferentiated status of the cells and is downregulated upon differentiation. Si-RNA mediated knockdown of Nanog in ES cells induces lineage differentiation. Overexpression of Nanog allows for the leukemia inhibitory factor (LIF)-independent self-renewal in undifferentiated ES cells. Nanog functionally works with OCT4 and SOX2 to support pluripotency and self-renewal in ES cells and the expression of Nanog is regulated by OCT4/SOX2 heterodimers. Expression of Nanog has been detected in several types of tumor cells. The expression level of Nanog in cancer cells has been shown to be correlated with the malignancy of cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length NANOG recombinant protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted at 4°C
Application

WB - Quality tested
ICFC, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml (1:200-1:1000 dilution). For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Product Citations
  1. Houweling PJ, et al. 2021. Stem Cell Research. 54:102429. PubMed
  2. Bozaoglu K, et al. 2020. Stem Cell Res. 49:102013. PubMed
RRID
AB_2564574 (BioLegend Cat. No. 674201)
AB_2564574 (BioLegend Cat. No. 674202)

Antigen Details

Structure
305 amino acids with a predicted molecular weight of approximately 35 kD and contains a homeobox domain that is responsible for DNA binding.
Interaction
Interacts with ZFP281, SMAD1, and SALL4.
Cell Type
Embryonic Stem Cells
Biology Area
Cell Biology, Immunology, Stem Cells, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Yin D, et al. 2012. Int. J. Mol. Med. 29:587.
2. Villasante A, et al. 2011. Cell Cycle 10:1488.
3. Theunissen TW, et al. 2011. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 366:2222.
4. Pan G, et al. 2007. Cell Res. 17:42.
5. Kashyap V, et al. 2009. Stem Cells Dev. 18:1093.
6. Silva J, et al. 2009. Cell 138:722.

Gene ID
79923 View all products for this Gene ID
UniProt
View information about Nanog on UniProt.org
Go To Top Version: 3    Revision Date: 06.20.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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