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APC anti-mouse/rat CD61 Antibody

Pricing & Availability
Clone
2C9.G2 (HMβ3-1) (See other available formats)
Regulatory Status
RUO
Other Names
β3 integrin, Integrin β3 chain, gpIIIa
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
2C9_APC_CD61_Antibody_FC_120712
C57BL/6 mouse bone marrow cells were stained with CD61 (clone 2C9.G2) APC (filled histogram) or Armenian hamster IgG APC isotype control (open histogram). Data shown was gated on myeloid cell population.
  • 2C9_APC_CD61_Antibody_FC_120712
    C57BL/6 mouse bone marrow cells were stained with CD61 (clone 2C9.G2) APC (filled histogram) or Armenian hamster IgG APC isotype control (open histogram). Data shown was gated on myeloid cell population.
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
104315 25 µg 102 CHF
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104316 100 µg 232 CHF
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Description

CD61 is a 110 kD integrin β chain also known as β3 integrin or gpIIIa. It associates with the integrin αv chain (CD51) to form the vitronectin receptor. In addition, CD61 can associate with the integrin αIIb chain (CD41) to form the gpIIb/IIIa complex. CD61 is expressed on platelets, megakaryocytes, endothelium, smooth muscle, a subset of B cells, myeloid cells, osteoclasts, and mast cells. CD61, in conjunction with CD41 or CD51, mediates adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, and von Willebrand factor. Leukocyte-endothelial adhesion is mediated by the binding of αv/β3 integrin or vitronectin receptor to CD31 (PECAM-1).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Vitronectin receptor protein from the mouse T-cell hybridoma 2B4.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 104325 and 104326).

Application References

(PubMed link indicates BioLegend citation)
  1. Kieffer N, et al. 1990. Annu. Rev. Cell Biol. 6:329. (Block)
  2. Piali L, et al. 1995. J. Cell Biol. 130:451. (Block)
  3. Ashkar S, et al. 2000. Science 287:860. (Block)
  4. Schultz JF, et al. 1995. J. Biol. Chem. 270:11522. (Block)
  5. Moulder K, et al. 1991. J. Exp. Med. 173:343. (Activ)
  6. Carlson TR, et al. 2008.135:2193. PubMed
  7. Yamaji D, et al. 2009. Genes Dev. 23:2382. PubMed
Product Citations
  1. Lan HT, et al. 2023. Evid Based Complement Alternat Med. 2022:6606423. PubMed
  2. Li X, et al. 2022. Cell. 185:1709. PubMed
  3. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  4. Dignum T, et al. 2021. Cell Rep. 36:109675. PubMed
  5. Flommersfeld S, et al. 2021. Immunity. :. PubMed
  6. Yamamoto M, et al. 2019. Commun Biol. 2:292. PubMed
  7. Pervolarakis N, et al. 2020. Cell Rep. 108273:33. PubMed
  8. Ni T, et al. 2016. Nat Cell Biol. 18:1221-1232. PubMed
  9. Lucotti S, et al. 2019. J Clin Invest. 130:1845. PubMed
RRID
AB_2561733 (BioLegend Cat. No. 104315)
AB_2561733 (BioLegend Cat. No. 104316)

Antigen Details

Structure
Integrin family, 110 kD
Distribution

Platelets, megakaryocytes, endothelium, smooth muscle, activated T cells, subset of B cells, monocytes, macrophages, osteoclasts, mast cells

Function
Attachment to matrix
Ligand/Receptor
Fibrinogen, von Willebrand factor, fibronectin
Cell Type
B cells, Endothelial cells, Macrophages, Mast cells, Megakaryocytes, Monocytes, Osteoclasts, Platelets, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press.
2. Phillips DR, et al. 1991. Cell. 65:359.
3. Felding-Habermann B, et al. 1993. Curr Opinion Cell Biol. 5:864.

Gene ID
16416 View all products for this Gene ID 29302 View all products for this Gene ID
UniProt
View information about CD61 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12.12.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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