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PE anti-mouse/rat CD61 Antibody

Pricing & Availability
Clone
2C9.G2 (HMβ3-1) (See other available formats)
Regulatory Status
RUO
Other Names
β3 integrin, Integrin β3 chain, gpIIIa
Isotype
Armenian Hamster IgG
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Product Citations
publications
2C9dotG2_PE_090707
C57BL/6 mouse bone marrow cells stained with 2C9.G2 PE
  • 2C9dotG2_PE_090707
    C57BL/6 mouse bone marrow cells stained with 2C9.G2 PE
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104307 50 µg 122 CHF
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104308 200 µg 309 CHF
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Description

CD61 is a 110 kD integrin β chain also known as β3 integrin or gpIIIa. It associates with the integrin αv chain (CD51) to form the vitronectin receptor. In addition, CD61 can associate with the integrin αIIb chain (CD41) to form the gpIIb/IIIa complex. CD61 is expressed on platelets, megakaryocytes, endothelium, smooth muscle, a subset of B cells, myeloid cells, osteoclasts, and mast cells. CD61, in conjunction with CD41 or CD51, mediates adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, and von Willebrand factor. Leukocyte-endothelial adhesion is mediated by the binding of αv/β3 integrin or vitronectin receptor to CD31 (PECAM-1).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Vitronectin receptor protein from the mouse T-cell hybridoma 2B4.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 104325 and 104326).

Application References

(PubMed link indicates BioLegend citation)
  1. Kieffer N, et al. 1990. Annu. Rev. Cell Biol. 6:329. (Block)
  2. Piali L, et al. 1995. J. Cell Biol. 130:451. (Block)
  3. Ashkar S, et al. 2000. Science 287:860. (Block)
  4. Schultz JF, et al. 1995. J. Biol. Chem. 270:11522. (Block)
  5. Moulder K, et al. 1991. J. Exp. Med. 173:343. (Activ)
  6. Carlson TR, et al. 2008.135:2193. PubMed
  7. Yamaji D, et al. 2009. Genes Dev. 23:2382. PubMed
Product Citations
  1. Laura C Burzynski et al. 2019. Immunity. 50(4):1033-1042 . PubMed
  2. Huang TC, et al. 2021. Nature. 600:737. PubMed
  3. Kang YA, et al. 2023. J Exp Med. 220:. PubMed
  4. Gawish R, et al. 2022. Elife. 11:. PubMed
  5. Zhang J, et al. 2018. Nature. 562:595. PubMed
  6. Li H, et al. 2021. Nat Commun. 12:2773. PubMed
  7. Cordeiro O, et al. 2016. PLoS One. 11: 0151848. PubMed
  8. Wang X, et al. 2021. Cell Res. 31:526. PubMed
  9. Aizawa E, et al. 2022. Stem Cell Reports. 17:43. PubMed
  10. Bonnardel J, et al. 2019. Immunity. 51:638. PubMed
  11. Lin J, et al. 2021. Mol Med. 27:149. PubMed
  12. Horiguchi H, et al. 2019. Genes Dev. 33:1641. PubMed
  13. Dravis C et al. 2018. Cancer cell. 34(3):466-482 . PubMed
  14. Bleckwehl T, et al. 2021. Nat Commun. 12:5771. PubMed
  15. Lyu C, et al. 2022. Cancers (Basel). 14:. PubMed
  16. Gao Q, et al. 2021. Stem Cells Transl Med. 10:743. PubMed
  17. Manupati K, et al. 2022. Cancers (Basel). 14:. PubMed
  18. Yu S, et al. 2022. Nat Commun. 13:2756. PubMed
  19. Zhuo Y, et al. 2018. Oncol Rep. 40:2147. PubMed
  20. Kinoshita M, et al. 2020. Cell Stem Cell. . PubMed
  21. Nasser H, et al. 2020. Cell Death Discov. 0.29375. PubMed
  22. Morgani SM, et al. 2021. Biology Open. 10(5):. PubMed
  23. Saboya M, et al. 2020. Alcohol Clin Exp Res. 44:831. PubMed
  24. Pele T, et al. 2021. Int J Mol Sci. 22:. PubMed
  25. Vojtek M, et al. 2021. Life Sci Alliance. 4:. PubMed
RRID
AB_313084 (BioLegend Cat. No. 104307)
AB_313084 (BioLegend Cat. No. 104308)

Antigen Details

Structure
Integrin family, 110 kD
Distribution

Platelets, megakaryocytes, endothelium, smooth muscle, activated T cells, subset of B cells, monocytes, macrophages, osteoclasts, mast cells

Function
Attachment to matrix
Ligand/Receptor
Fibrinogen, von Willebrand factor, fibronectin
Cell Type
B cells, Endothelial cells, Macrophages, Mast cells, Megakaryocytes, Monocytes, Osteoclasts, Platelets, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press.
2. Phillips DR, et al. 1991. Cell. 65:359.
3. Felding-Habermann B, et al. 1993. Curr Opinion Cell Biol. 5:864.

Gene ID
16416 View all products for this Gene ID 29302 View all products for this Gene ID
UniProt
View information about CD61 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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