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Brilliant Violet 421™ anti-human CD107a (LAMP-1) Antibody

Pricing & Availability
Clone
H4A3 (See other available formats)
Regulatory Status
RUO
Workshop
P PR-63; BP 473; P P008
Other Names
Lysosome-Associated Membrane Protein 1, LGP-120, LAMP-1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
H4A3_BV421_041411
Thrombin-activated human peripheral blood platelets were stained with CD107a (clone H4A3) Brilliant Violet 421™ (filled histogram) or mouse IgG1,κ Brilliant Violet 421™ isotype control (open histogram).
  • H4A3_BV421_041411
    Thrombin-activated human peripheral blood platelets were stained with CD107a (clone H4A3) Brilliant Violet 421™ (filled histogram) or mouse IgG1,κ Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
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328625 25 tests 190 CHF
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328626 100 tests 396 CHF
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Description

CD107a, also known as Lysosome-Associated Membrane Protein 1 (LAMP-1) or LGP-120, is a 110-140 kD type I membrane glycoprotein. Mature CD107a is heavily glycosylated from a 40 kD core protein. This molecule is located on the luminal side of lysosomes. Upon activation, CD107a is transferred to the cell membrane surface of activated platelets, activated lymphocytes, macrophages, epithelial cells, endothelial cells, and some tumor cells. CD107a has been suggested to play a role in the protection of lysosomal membrane from lysosomal hydrolases which is involved in cell adhesion and regulation of tumor metastasis, and mediates autoimmune disease progression. CD107a is a ligand for galaptin and E-selectin. Surface expression of LAMP-1 has been shown to correlate with CD8+ T cell and NK cell cytotoxicity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human adult adherent peripheral blood cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting8, immunohistochemical staining2, immunofluorescence5,7, and immunoprecipitation5.

This antibody is specific to human LAMP-1. Positive control: Hela cells; LAMP-1 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation.

Application References

(PubMed link indicates BioLegend citation)
  1. Misse D, et al. 1999. Blood 93:2454.
  2. Furuta K, et al. 2001. Am. J. Pathol. 159:449. (IHC)
  3. Watanabe A, et al. 2011. J. Biol. Chem. 286:10702. PubMed
  4. Baron Gaillard CL, et al. 2011. Mol. Cell. Biol. 22:5459. PubMed
  5. Hauck CR and Meyer TF. 1997. FEBS Lett. 405:86. (IF, IP)
  6. De Keersmaecker B, et al. 2012. J. Virol. 86:9351. PubMed
  7. Knodler LA, et al. 2010. P. Natl. Acad. Sci. USA. 107:17733. (IF)
  8. Oh J, et al. 2000. Hum. Mol. Genet. 9:375. (WB)
  9. Salio M, et al. 2013 PNAS. 110:4753. PubMed
Product Citations
  1. Bhatt RS, et al. 2021. Cancer Immunol Res. 9:156. PubMed
  2. Parasar P, et al. 2022. Am J Reprod Immunol. 88:e13614. PubMed
  3. Pinto C, et al. 2023. iScience. 26:106381. PubMed
  4. Yan X, et al. 2023. Cell Mol Life Sci. 80:189. PubMed
  5. Keersmaecker B, et al. 2012. J Virol. J. Virol. PubMed
  6. Kuang Z, et al. 2020. Antib Ther. 3:227. PubMed
  7. Tebas P, et al. 2021. J Clin Invest. 131:. PubMed
  8. Sim MJW, et al. 2019. Proc Natl Acad Sci U S A. 116:12964. PubMed
  9. Jackson E, et al. 2017. PLoS One. 10.1371/journal.pone.0185160. PubMed
  10. Rice TF, et al. 2021. EBioMedicine. 72:103612. PubMed
  11. Braun M, et al. 2014. PLoS Pathog. 10:1004521. PubMed
  12. Rider P, et al. 2015. J Immunol. 195: 1705-1712. PubMed
  13. Johnson JK, et al. 2022. Methods Mol Biol. 2470:641. PubMed
  14. Roberts E, et al. 2016. PLoS One. 11:e0168488. PubMed
  15. Chalan P, et al. 2016. J Rheumatol. 43: 1008 - 1016. PubMed
  16. Terawaki S, et al. 2015. J Cell Biol. 210: 1133-1152. PubMed
  17. Sagebiel AF, et al. 2019. Nat Commun. 10:975. PubMed
  18. Dai HS et al. 2017. Immunity. 47(1):159-170 . PubMed
  19. Motozono C, et al. 2021. Cell Host Microbe. . PubMed
  20. Körner C et al. 2017. Cell host & microbe. 22(1):111-119 . PubMed
  21. Clayton KL, et al. 2021. Cell Host Microbe. 29(3):435-447.e9. PubMed
RRID
AB_10899581 (BioLegend Cat. No. 328625)
AB_10899581 (BioLegend Cat. No. 328626)

Antigen Details

Structure
LAMP-1 is a 417 amino acid protein with a molecular mass of 45 kD.
Distribution

Macrophages, epithelial cells, endothelial cells, some tumor cells; located on the luminal side of lysosomes or on the surface of cell membranes

Function
Protect lysosomal membrane from lysosomal hydrolases, adhesion
Ligand/Receptor
Galaptin
Cell Type
Endothelial cells, Epithelial cells, Macrophages
Biology Area
Cell Biology, Immunology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Sarafian V, et al. 2006. Arch. Dermatol. Res. 298:7381.
2. Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. New York:Oxford University Press.
3. Sawada R, et al. 1993. J. Biol. Chem. 268:12675.
4. Chen JW, et al. 1988. J. Biol. Chem. 263:8754.
5. Chen JW, et al. 1986. Biochem. Soc. Symp. 51:97112.

Gene ID
3916 View all products for this Gene ID
UniProt
View information about CD107a on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 2    Revision Date: 10.22.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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