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PE anti-human CD107a (LAMP-1) Antibody

Pricing & Availability
Clone
H4A3 (See other available formats)
Regulatory Status
RUO
Workshop
P PR-63; BP 473; P P008
Other Names
Lysosome-Associated Membrane Protein 1, LGP-120, LAMP-1
Isotype
Mouse IgG1, κ
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Product Citations
publications
H4A3_PE_090808.jpg
Thrombin-activated human peripheral blood platelets were stained with CD107a (clone H4A3) PE (pink histogram) or mouse IgG1, κ PE (blue histogram).
  • H4A3_PE_090808.jpg
    Thrombin-activated human peripheral blood platelets were stained with CD107a (clone H4A3) PE (pink histogram) or mouse IgG1, κ PE (blue histogram).
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328607 25 tests 108 CHF
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328608 100 tests 252 CHF
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Description

CD107a, also known as Lysosome-Associated Membrane Protein 1 (LAMP-1) or LGP-120, is a 110-140 kD type I membrane glycoprotein. Mature CD107a is heavily glycosylated from a 40 kD core protein. This molecule is located on the luminal side of lysosomes. Upon activation, CD107a is transferred to the cell membrane surface of activated platelets, activated lymphocytes, macrophages, epithelial cells, endothelial cells, and some tumor cells. CD107a has been suggested to play a role in the protection of lysosomal membrane from lysosomal hydrolases which is involved in cell adhesion and regulation of tumor metastasis, and mediates autoimmune disease progression. CD107a is a ligand for galaptin and E-selectin. Surface expression of LAMP-1 has been shown to correlate with CD8+ T cell and NK cell cytotoxicity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human adult adherent peripheral blood cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting8, immunohistochemical staining2, immunofluorescence5,7, and immunoprecipitation5.

This antibody is specific to human LAMP-1. Positive control: Hela cells; LAMP-1 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation.

Application References

(PubMed link indicates BioLegend citation)
  1. Misse D, et al. 1999. Blood 93:2454.
  2. Furuta K, et al. 2001. Am. J. Pathol. 159:449. (IHC)
  3. Watanabe A, et al. 2011. J. Biol. Chem. 286:10702. PubMed
  4. Baron Gaillard CL, et al. 2011. Mol. Cell. Biol. 22:5459. PubMed
  5. Hauck CR and Meyer TF. 1997. FEBS Lett. 405:86. (IF, IP)
  6. De Keersmaecker B, et al. 2012. J. Virol. 86:9351. PubMed
  7. Knodler LA, et al. 2010. P. Natl. Acad. Sci. USA. 107:17733. (IF)
  8. Oh J, et al. 2000. Hum. Mol. Genet. 9:375. (WB)
  9. Salio M, et al. 2013 PNAS. 110:4753. PubMed
Product Citations
  1. Qin L, et al. 2022. Sci Adv. 8:eabn3774. PubMed
  2. Jo DH, et al. 2023. Mol Ther Methods Clin Dev. 29:173. PubMed
  3. Liu R, et al. 2022. Aging Dis. 13:1576. PubMed
  4. Afolabi LO, et al. 2021. Front Immunol. 12:701671. PubMed
  5. Willcox CR, et al. 2020. Immunity. 51(5):813-825.e4.. PubMed
  6. Lamichhane R, et al. 2020. Eur J Immunol. 50:178. PubMed
  7. Chen D, et al. 2021. Dev Cell. 56:3250. PubMed
  8. Kim JT, et al. 2022. Nat Commun. 13:121. PubMed
  9. Gao Y, et al. 2021. Oncogenesis. 10:62. PubMed
  10. Izumi K, et al. 2021. Immunohorizons. 5:687. PubMed
  11. Stevenson EM, et al. 2022. Nat Commun. 13:4888. PubMed
  12. Karampatzakis A, et al. 2021. Front Immunol. 12:641521. PubMed
  13. Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed
  14. Sibener LV et al. 2018. Cell. 174(3):672-687 . PubMed
  15. Chandran P, et al. 2014. J Leukoc Biol. 96:633. PubMed
  16. Laroni A, et al. 2016. J Autoimmun. 72:8-18. PubMed
  17. Palazzo A, et al. 2018. J Immunol. 200:2304. PubMed
  18. Yao X, et al. 2020. Adv Sci (Weinh). 7:1902938. PubMed
  19. Su X, et al. 2022. J Transl Med. 20:378. PubMed
  20. Schanin J, et al. 2022. Commun Biol. 5:1226. PubMed
  21. Castro I, et al. 2016. J Virol. 90: 5280 - 5291. PubMed
  22. Oyer JL, et al. 2022. Front Immunol. 13:861681. PubMed
  23. Hirama T, et al. 2021. JCI Insight. 6:. PubMed
  24. Pugsley H, et al. 2017. Methods. 112:147-156. PubMed
  25. Lettau M, et al. 2018. Int Immunol. 30:215. PubMed
  26. Pollack RA, et al. 2017. Cell Host Microbe. 1.218055556. PubMed
  27. Li Y, et al. 2022. J Immunol. 208:347. PubMed
  28. Heard A, et al. 2022. Nat Commun. 13:3367. PubMed
  29. Pahl JHW, et al. 2018. Cancer Immunol Res. 0.609027778. PubMed
  30. Katsuyama E, et al. 2020. Cell Reports. 30(1):112-123.e4.. PubMed
  31. Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed
  32. Gambacorta V, et al. 2022. Cancer Discov. . PubMed
  33. Pomplun NL, et al. 2021. Cytometry A. 99:278. PubMed
  34. Christensen SR, et al. 2019. J Virol. 93:8. PubMed
  35. Liu CF, et al. 2017. Front Immunol. 1.445833333. PubMed
  36. Plum T, et al. 2020. Immunity. 52(2):404-416. PubMed
  37. Mo YQ, et al. 2022. J Clin Invest. . PubMed
  38. Shen C, et al. 2021. BMC Med. 19:283. PubMed
  39. Chen PM, et al. 2022. Sci Adv. 8:eabo4271. PubMed
  40. Li M, et al. 2020. Virol Sin. 35:588. PubMed
  41. Sadallah S, et al. 2016. J Immunol. 197: 1663 - 1671. PubMed
  42. Huang W, et al. 2012. Cell Res. 22:473. PubMed
  43. Lamichhane R et al. 2019. Cell Rep. 28(12):3061-3076 . PubMed
RRID
AB_1186040 (BioLegend Cat. No. 328607)
AB_1186040 (BioLegend Cat. No. 328608)

Antigen Details

Structure
LAMP-1 is a 417 amino acid protein with a molecular mass of 45 kD.
Distribution

Macrophages, epithelial cells, endothelial cells, some tumor cells; located on the luminal side of lysosomes or on the surface of cell membranes

Function
Protect lysosomal membrane from lysosomal hydrolases, adhesion
Ligand/Receptor
Galaptin
Cell Type
Endothelial cells, Epithelial cells, Macrophages
Biology Area
Cell Biology, Immunology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Sarafian V, et al. 2006. Arch. Dermatol. Res. 298:7381.
2. Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. New York:Oxford University Press.
3. Sawada R, et al. 1993. J. Biol. Chem. 268:12675.
4. Chen JW, et al. 1988. J. Biol. Chem. 263:8754.
5. Chen JW, et al. 1986. Biochem. Soc. Symp. 51:97112.

Gene ID
3916 View all products for this Gene ID
UniProt
View information about CD107a on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 10.22.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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