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FITC anti-human CD107a (LAMP-1) Antibody

Pricing & Availability
Clone
H4A3 (See other available formats)
Regulatory Status
RUO
Workshop
P PR-63; BP 473; P P008
Other Names
Lysosome-Associated Membrane Protein 1, LGP-120, LAMP-1
Isotype
Mouse IgG1, κ
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Product Citations
publications
H4A3_FITC_090808.jpg
Thrombin-activated human peripheral blood platelets were stained with CD107a (clone H4A3) FITC (red histogram) or mouse IgG1, κ FITC (green histogram).
  • H4A3_FITC_090808.jpg
    Thrombin-activated human peripheral blood platelets were stained with CD107a (clone H4A3) FITC (red histogram) or mouse IgG1, κ FITC (green histogram).
Compare all formats See FITC spectral data
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328605 25 tests 98 CHF
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328606 100 tests 226 CHF
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Description

CD107a, also known as Lysosome-Associated Membrane Protein 1 (LAMP-1) or LGP-120, is a 110-140 kD type I membrane glycoprotein. Mature CD107a is heavily glycosylated from a 40 kD core protein. This molecule is located on the luminal side of lysosomes. Upon activation, CD107a is transferred to the cell membrane surface of activated platelets, activated lymphocytes, macrophages, epithelial cells, endothelial cells, and some tumor cells. CD107a has been suggested to play a role in the protection of lysosomal membrane from lysosomal hydrolases which is involved in cell adhesion and regulation of tumor metastasis, and mediates autoimmune disease progression. CD107a is a ligand for galaptin and E-selectin. Surface expression of LAMP-1 has been shown to correlate with CD8+ T cell and NK cell cytotoxicity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human adult adherent peripheral blood cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting8, immunohistochemical staining2, immunofluorescence5,7, and immunoprecipitation5.

This antibody is specific to human LAMP-1. Positive control: Hela cells; LAMP-1 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation.

Application References

(PubMed link indicates BioLegend citation)
  1. Misse D, et al. 1999. Blood 93:2454.
  2. Furuta K, et al. 2001. Am. J. Pathol. 159:449. (IHC)
  3. Watanabe A, et al. 2011. J. Biol. Chem. 286:10702. PubMed
  4. Baron Gaillard CL, et al. 2011. Mol. Cell. Biol. 22:5459. PubMed
  5. Hauck CR and Meyer TF. 1997. FEBS Lett. 405:86. (IF, IP)
  6. De Keersmaecker B, et al. 2012. J. Virol. 86:9351. PubMed
  7. Knodler LA, et al. 2010. P. Natl. Acad. Sci. USA. 107:17733. (IF)
  8. Oh J, et al. 2000. Hum. Mol. Genet. 9:375. (WB)
  9. Salio M, et al. 2013 PNAS. 110:4753. PubMed
Product Citations
  1. Pymm P, et al. 2022. Cell Rep. 38:110449. PubMed
  2. Yen M, et al. 2022. Cell. 185:1414. PubMed
  3. Khan A, et al. 2015. J Virol. 89:3776. PubMed
  4. Xu H, et al. 2019. Cell Stem Cell. 24:566. PubMed
  5. Chen L, et al. 2023. Immunology. 169:204. PubMed
  6. Liu L, et al. 2023. J Exp Clin Cancer Res. 42:85. PubMed
  7. Luo H, et al. 2023. Bio Protoc. 13:e4693. PubMed
  8. Bilich T, et al. 2021. Cancer Discov. 11:1982. PubMed
  9. Jonescheit H, et al. 2020. Cells. :9. PubMed
  10. Myers JA, et al. 2022. JCI Insight. :. PubMed
  11. Bawazir M, et al. 2022. Front Immunol. 13:1033794. PubMed
  12. Nguyen MAT, et al. 2020. Immunohorizons. 0.465277778. PubMed
  13. Bauer J, et al. 2022. Nat Commun. 13:6401. PubMed
  14. Yu H, et al. 2021. Mol Ther Oncolytics. 21:158. PubMed
  15. van der Ploeg K, et al. 2022. Cell Rep Med. 3:100640. PubMed
  16. Cribbs AP, et al. 2021. Front Immunol. 12:626255. PubMed
  17. Hendricks D, et al. 2014. J Immunol. 192:4492. PubMed
  18. Guiliani E, et al. 2017. Immunol Lett. 10.1016/j.imlet.2017.08.014. PubMed
  19. Bertulli C, et al. 2020. Int J Mol Sci. 21:. PubMed
  20. Heitmann JS, et al. 2021. Nature. Online ahead of print. PubMed
  21. Bilich T, et al. 2021. Science Translational Medicine. 13(590):. PubMed
  22. Bilich T, et al. 2020. Blood Cancer J. 10:24. PubMed
  23. Dockry E, et al. 2018. Oncoimmunology. 7:e1428156. PubMed
  24. Tai Y, et al. 2012. Blood. 119:2074. PubMed
  25. Zhang X, et al. 2021. Front Immunol. 12:602492. PubMed
  26. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  27. Davis Z, et al. 2016. PLoS Pathog. 12: 1005421. PubMed
  28. Ferry GM, et al. 2022. Front Immunol. 13:863155. PubMed
  29. Schlecker E, et al. 2014. Cancer Res. 74:3429. PubMed
  30. Burel J, et al. 2016. PLoS Negl Trop Dis. 10:e0005031. PubMed
  31. Lettau M, et al. 2018. Int Immunol. 30:215. PubMed
  32. Khan AA, et al. 2018. J Virol. 92:e00535. PubMed
  33. Pahl JHW, et al. 2018. Cancer Immunol Res. 0.609027778. PubMed
  34. Felices M, et al. 2018. JCI Insight. 3. PubMed
  35. Srivastava R, et al. 2015. J Immunol. 194:2232. PubMed
  36. Kwant-Mitchell A, et al. 2009. PLoS One. 4:e8379. PubMed
  37. Bennstein SB, et al. 2020. eLife. 9:e55232.. PubMed
RRID
AB_1186036 (BioLegend Cat. No. 328605)
AB_1186036 (BioLegend Cat. No. 328606)

Antigen Details

Structure
LAMP-1 is a 417 amino acid protein with a molecular mass of 45 kD.
Distribution

Macrophages, epithelial cells, endothelial cells, some tumor cells; located on the luminal side of lysosomes or on the surface of cell membranes

Function
Protect lysosomal membrane from lysosomal hydrolases, adhesion
Ligand/Receptor
Galaptin
Cell Type
Endothelial cells, Epithelial cells, Macrophages
Biology Area
Cell Biology, Immunology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Sarafian V, et al. 2006. Arch. Dermatol. Res. 298:7381.
2. Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. New York:Oxford University Press.
3. Sawada R, et al. 1993. J. Biol. Chem. 268:12675.
4. Chen JW, et al. 1988. J. Biol. Chem. 263:8754.
5. Chen JW, et al. 1986. Biochem. Soc. Symp. 51:97112.

Gene ID
3916 View all products for this Gene ID
UniProt
View information about CD107a on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 10.22.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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