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Purified anti-Cytokeratin 6 Antibody

Pricing & Availability
Clone
W17006B (See other available formats)
Regulatory Status
RUO
Other Names
Keratin, type II cytoskeletal 6, Cytokeratin-6, CK-6, Keratin-6, KRT6
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W17006B_PURE_Cytokeratin-6_Antibody_1_WB_042618
Total cell lysate (15 µg protein) from HeLa (negative control), HaCaT, A431 and NIH3T3 cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:1000 (0.5 µg/mL) diluted purified anti-Cytokeratin 6 (clone W17006B, upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP rat-IgG secondary antibody (Cat. No. 405405). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1, Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
  • W17006B_PURE_Cytokeratin-6_Antibody_1_WB_042618
    Total cell lysate (15 µg protein) from HeLa (negative control), HaCaT, A431 and NIH3T3 cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:1000 (0.5 µg/mL) diluted purified anti-Cytokeratin 6 (clone W17006B, upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP rat-IgG secondary antibody (Cat. No. 405405). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1, Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
  • W17006B_PURE_Cytokeratin-6_Antibody_2_ICC_042618
    HaCaT cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:500 diluted (1 µg/ml, Figure A) rat IgG2b isotype control (Cat. No. 400601)or 1:500 (1 µg/ml, Figure B), 1:2000 (0.25 µg/ml, Figure C) and 1:5000 (0.1 µg/ml, Figure D) diluted anti-Cytokeratin 6 overnight at 4° followed by 1:250 diluted (2 µg/ml) Alexa Fluor® 488 (green) conjugated goat anti-rat IgG (Cat. No. 405418) antibody for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • W17006B_PURE_Cytokeratin-6_Antibody_3_IHC-P_072122
    IHC staining using purified anti-cytokeratin 6 antibody (clone W17006B) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using Tris-EDTA buffer (10mM Tris, 1mM EDTA, pH 9.0), the tissue was incubated with 10 µg/mL of antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-rat IgG antibody (magenta) (Cat. No. 405416) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 20X objective. Scalebar = 50 µM.
Cat # Size Price Quantity Check Availability Save
606101 25 µg 101 CHF
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606102 100 µg 253 CHF
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Description

Keratins are main structural proteins of epithelial cells and form a cytoplasmic intermediate filament network, which is required for cell integrity and protecting cells from stress or injury. Keratin 6 (KRT6), also known as cytokeratin-6 or CK-6, is a type II intermediate filament protein constitutively expressed in all major types of epithelial appendages, such as hair, nail, and glands. Expression of KRT6 is induced by cell injury, infection, and disease. KRT6 has been identified as a marker of mammary gland progenitor cells. Defects in KRT6 genes have been associated with the inherited disorder pachyonychia congenital (PC), leading to structural abnormalities of nail, palms, and soles.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human cytokeratin 6A peptide (2-18 a.a.) conjugated to KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/ml (1:500-1:5000 dilution). For immunocytochemistry, a concentration range of 0.1 - 1.0 µg/ml (1:500-1:5000 dilution) is recommended. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For immunohistochemistry, the suggested antigen retrieval buffer is either Tris-EDTA or sodium citrate (Cat. No. 420901).

Product Citations
  1. Koliakou E, et al. 2022. Int J Mol Sci. 23: . PubMed
RRID
AB_2734500 (BioLegend Cat. No. 606101)
AB_2734500 (BioLegend Cat. No. 606102)

Antigen Details

Structure
564 amino acids with a predicted molecular weight of 60 kD. Type II intermediate filament.
Distribution

Cytoplasm

Function
KRT6 is a type II intermediate filament protein required for cell integrity and protecting cells from stress or injury.
Interaction
KRT6 interacts with KRT16, KRT17, and TCHP.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References
  1. Arumugam A, et al. 2014. Biochem. Biophys. Res. Commun. 451:394.
  2. Cao L, et al. 2010. J. Appl. Toxicol. 30:416.
  3. Gu LH, Coulombe PA. 2008. Am. J. Pathol. 173:752.
  4. Wong P, Coulombe PA. 2003. J. Cell Biol. 163:327.
  5. Lin MT, et al. 1999. Exp. Dermatol. 8:115.
  6. Takahashi K, et al. 1995. J. Biol. Chem. 4:18581.
Gene ID
3853 View all products for this Gene ID
UniProt
View information about Cytokeratin 6 on UniProt.org
Go To Top Version: 2    Revision Date: 07.21.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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