Purified anti-human CD163 Antibody anti-CD163

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Clone: RM3/1 (See other available formats)
Regulatory Status: RUO
Other Names: GHI/61, M130, RM3/1, p155, Hemoglobin/haptoglobin complex receptor, Macrophage-associated antigen, ED2(rat), Macrophage marker
Isotype: Mouse IgG1, κ
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RM3slash1_Purified_CD163_Antibody_FC_032014 — IL-10-stimulated (overnight) human peripheral blood monocytes were stained with purified CD163 (clone RM3/1, filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG FITC.

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326502

100 µg

118 CHF

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Description

CD163 is a member of the group B scavenger receptor cysteine-rich superfamily, also known as GHI/61, M130, RM3/1, p155, hemoglobin-haptoglobin complex receptor, or macrophage-associated antigen. It is a 134 kD (non-reduced)/155 kD (reduced) glycoprotein primarily expressed on macrophages, Kuffer cells, monocytes, subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells. CD163 binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages. Membrane CD163 can be cleaved by metalloproteinases (MMP), resulting in a soluble form. Elevated serum level of sCD163 has been implicated in many kinds of inflammation diseases.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human monocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
ICC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone RM3/1 binds to domain 9 of CD163. Additional reported applications (for the relevant formats) include: immunofluorescence⁷.
RRID: AB_893267 (BioLegend Cat. No. 326502)

Antigen Details

Structure: 134 kD (non-reduced)/155 kD (reduced) glycoprotein, Scavenger receptor superfamily
Distribution: Monocytes, macrophages, Kuffer cells, subset of dendritic cells, subset of hematopoietic stem/progenitor cells
Function: Clearance of haptoglobin-hemoglobin complex, regulation of cytokine production by macrophages
Ligand/Receptor: Haptoglobin-hemoglobin complex, TWEAK
Cell Type: Dendritic cells, Hematopoietic stem and progenitors, Macrophages, Monocytes
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Roth J, et al. 1994. Transolantation. 57:127.
2. Van den Heuvel MM, et al.1999. J. Leukoc. Biol. 66:858.
3. Sulahian TH, et al. 2000. Cytokines 12:1312.
4. Fabriek BO, et al. 2007. J. Neuroimmunol. 187:179.
Gene ID: 9332 View all products for this Gene ID
UniProt: View information about CD163 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

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Description	Clone	Applications
Purified anti-human CD163	RM3/1	FC,ICC
PE anti-human CD163	RM3/1	FC
Alexa Fluor® 647 anti-human CD163	RM3/1	FC
APC anti-human CD163	RM3/1	FC
PerCP/Cyanine5.5 anti-human CD163	RM3/1	FC
PE/Cyanine7 anti-human CD163	RM3/1	FC

Certificate of Analysis

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Go To Top Version: 4 Revision Date: 05.07.2014

For Research Use Only. Not for diagnostic or therapeutic use.

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