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Purified anti-human CD284 (TLR4) Antibody

Pricing & Availability
Clone
HTA125 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
Toll-like Receptor 4, TLR4, TLR-4, CD284
Isotype
Mouse IgG2a, κ
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Product Citations
publications
HTA125_PE_071504
Human peripheral blood monocytes stained with HTA125 PE
  • HTA125_PE_071504
    Human peripheral blood monocytes stained with HTA125 PE
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312802 100 µg 197 CHF
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Description

Toll-like receptors are type I transmembrane signaling receptors. They are primordial pathogen-recognition proteins that function as sentinels for the innate immune system. TLR4, also known as CD284, is a 110 kD protein which is expressed on monocytes/macrophages, endothelial cells, and at low levels on B cells and granulocytes. In association with a secretory molecule, MD2, TLR4 has been recognized as critical for host recognition of bacterial LPS. HTA125 antibody is useful for flow cytometric analysis and is able to block LPS-induced cytokine production.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Guinea Pig
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Ba/F3 cell line expressing human TLR4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, ICC, WB, Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4, immunofluorescence microscopy6, Western blotting10, and in vitro blocking of LPS-induced cytokine production2,3,7,9. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 312806) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 312804) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 312813 & 312814). 

Application References

(PubMed link indicates BioLegend citation)
  1. Skimazu R, et al. 1999. J. Exp. Med. 189:1777.
  2. Wang R, et al. 2003. Hybrid Hybridomics 22:357. (Block)
  3. Wang JE, et al. 2001. Infect. Immun. 69:2402. (Block)
  4. Ishihara S, et al. 2004 J. Immunol. 173:1406. (IHC)
  5. Kawahara T, et al. 2001 Infect. Immun. 69:4382.
  6. Jiang Q, et al. 2000. J. Immunol. 165:3541. (IF)
  7. Sugawara S, et al. 2001. Infect. Immun. 69:4951. (Block)
  8. Chavakis E, et al. 2007. Circ. Res. 100:204. PubMed
  9. Bhattacharyya S, et al. 2007. Am. J. Physiol. Gastrointest Liver Physiol. doi:10.1152/ajpgi.00149. (Block) PubMed
  10. Baumgarten G, et al. 2001. J. Infectious. Dis. 183:1617.
Product Citations
  1. Waller KJ, et al. 2022. Clin Mol Hepatol. :. PubMed
  2. Feng J, et al. 2023. Vaccines (Basel). 11:. PubMed
  3. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  4. Manukyan G, et al. 2017. Oncotarget. 8:84889. PubMed
  5. Bai X, et al. 2022. BMC Immunol. 23:42. PubMed
  6. Heesters BA, et al. 2021. J Exp Med. 218:. PubMed
  7. Stephens M, et al. 2021. Sci Rep. 1.385416667. PubMed
  8. Yoon J, et al. 2020. Front Cell Dev Biol. 0.520138889. PubMed
  9. Chavakis E, et al. 2007. Circ Res. 100:204. PubMed
RRID
AB_314951 (BioLegend Cat. No. 312802)

Antigen Details

Structure
Type I transmembrane protein, Toll-like receptor family/IL-1R superfamily, 110 kD
Distribution

Monocytes/macrophages, endothelial cells, B cells and granulocytes

Function
Activates the innate immune system against Gram-negative bacterial pathogens by recognition of LPS
Ligand/Receptor
Bacterial LPS
Cell Type
B cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References

1. Skimazu R, et al. 1999. J. Exp. Med. 189:1777.

Gene ID
7099 View all products for this Gene ID
UniProt
View information about CD284 on UniProt.org
Go To Top Version: 6    Revision Date: 11.11.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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