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Purified anti-human CD31 Antibody

Pricing & Availability
Clone
WM59 (See other available formats)
Regulatory Status
RUO
Workshop
V P025
Other Names
PECAM-1, EndoCAM
Isotype
Mouse IgG1, κ
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Product Citations
publications
WM59_FITC
Human peripheral blood lymphocytes, monocytes and granulocytes stained with purified WM59, then detected with anti-mouse IgG FITC
  • WM59_FITC
    Human peripheral blood lymphocytes, monocytes and granulocytes stained with purified WM59, then detected with anti-mouse IgG FITC
  • WM59_Purified_CD31_Antibody_ELISA_112618
  • WM59_Purified_CD31_Antibody_ICC_112018
    HUVEC cells were fixed with 1% paraformaldehyde (PFA) and blocked with 5% fetal bovine serum for 30 minutes at room temperature. Then the cells were stained with 10 µg/ml of purified anti-CD31 (clone WM59) overnight, followed by 2.5 µg/ml DyLight™ 594 anti-mouse IgG for 2 hours at 4°C (red). Nuclei were counterstained with DAPI (blue). The image was captured by 20X objective.
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303101 25 µg 54 CHF
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303102 100 µg 102 CHF
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Description

CD31 is a 130-140 kD type I transmembrane glycoprotein also known as platelet endothelial cell adhesion molecule-1 (PECAM-1) or Endocam. It is expressed on monocytes, platelets, granulocytes, endothelial cells and lymphocyte subsets. CD31 has been reported to bind CD38 and be involved in wound healing, angiogenesis, and cellular migration in an inflammatory situation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ELISA Capture, ICC - Verified
IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. For ELISA Capture applications, a concentration of 1.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of CD31 recombinant protein ranging from 10 to 0.156 ng/mL is recommended for each ELISA plate. For immunocytochemistry, a concentration range of 2.5 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone WM59 has been reported to recognize the D2 extracellular portion of CD31.

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, immunohistochemical staining of acetone-fixed frozen tissue sections8, blocking of platelet aggregation3, and spatial biology (IBEX)11,12. Clone WM59 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 303143 & 303144).

The purified WM59 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotin anti-human CD31 antibody (Cat. No. 536604) antibody as the detection antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V Oxford University Press. New York.
  2. Muczynski KA, et al. 2003. J. Am. Soc. Nephrol. 14:1336. (IF)
  3. Wu XW, et al. 1997. Arterioscl. Throm. Vas. 17:3154. (Block)
  4. Nagano M, et al. 2007. Blood 110:151. (FC) PubMed
  5. MacFadyen JR, et al. 2005. FEBS Lett. 579:2569. PubMed
  6. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  7. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
  8. Wicki A, et al. 2012. Clin. Cancer Res. 18:454. (FC, IHC) PubMed
  9. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
  10. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110 (IF) PubMed
  11. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  12. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Yuge S, et al. 2022. Nat Commun. 13:2594. PubMed
  2. Setua S, et al. 2022. Sci Rep. 12:22191. PubMed
  3. Critchley WR, et al. 2023. J Cell Sci. 136:. PubMed
  4. Xie T, et al. 2021. Sci Adv. 7: . PubMed
  5. Robert J et al. 2017. eLife. 6 pii: e29595. PubMed
  6. Bloodworth NC, et al. 2018. Circ Res. 123:e51. PubMed
  7. Constantinescu-Bercu A, et al. 2020. eLife. 9:00. PubMed
  8. Fitzgerald W, et al. 2018. Am J Reprod Immunol. 80:e12860. PubMed
  9. Yoshihara S, et al. 2019. Front Immunol. 0.545833333. PubMed
  10. Vukusic K, et al. 2019. Stem Cells Dev. 28:1078. PubMed
  11. Olin A, et al. 2018. Cell. 174:1277. PubMed
  12. Yoshitomi Y, et al. 2017. J Cell Sci. 130:916. PubMed
  13. Nagano M, et al. 2007. Blood . 110:151. PubMed
  14. Boulanger MD, et al. 2022. PLoS One. 17:e0269316. PubMed
  15. Li X, et al. 2022. Cancer Cell Int. 22:157. PubMed
  16. Henrick BM, et al. 2021. Cell. . PubMed
  17. Lundqvist A, et al. 2016. PLoS One. 11: 0161629. PubMed
  18. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  19. Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
  20. Lee SH, et al. 2022. Nat Commun. 13:5461. PubMed
  21. Lakshmikanth T, et al. 2020. Cell Reports. 32(3):107923. PubMed
  22. Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed
  23. Fujiwara K, et al. 2020. Cancers (Basel). 12:. PubMed
  24. Gunasekaran M, et al. 2018. J Immunol. 200:2535. PubMed
  25. Ran LJ, et al. 2018. Mol Med Rep. 18:723. PubMed
  26. Robert J, et al. 2017. Mol Neurodegener. 12:60. PubMed
  27. Crawford LB, et al. 2021. J Virol. 95:. PubMed
  28. Li H, et al. 2020. Haematologica. 105:1206. PubMed
  29. Martin E, et al. 2020. JCI Insight. :5. PubMed
RRID
AB_314327 (BioLegend Cat. No. 303101)
AB_314327 (BioLegend Cat. No. 303102)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 130-140 kD
Distribution

Monocytes, platelets, granulocytes, endothelial cells, lymphocyte subset

Function
Cell adhesion, signal transduction
Ligand/Receptor
CD38
Cell Type
Endothelial cells, Granulocytes, Lymphocytes, Monocytes, Neutrophils, Platelets
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. DeLisser H, et al. 1994. Immunol. Today 15:490.
  2. Newman P, 1997. J. Clin. Invest. 99:3.
  3. Fawcett J, et al. 1995. J. Cell Biol. 128:1229.
Gene ID
5175 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 09.06.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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