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Purified anti-Keratin 1 Antibody (Previously Covance catalog# PRB-165P)

Pricing & Availability
Clone
Poly19056 (See other available formats)
Regulatory Status
RUO
Other Names
Keratin, type II cytoskeletal 1, K1, CK-1, keratin 86, cytokeratin-1, 67 kD cytokeratin, type-II keratin Kb1, keratin complex 2, basic, gene 1
Previously
Covance Catalog# PRB-165P
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Purified_Keratin-1_Antibody_IHC_021715
Double-label immunofluorescence analysis of developing mouse epidermis showing E18 epidermis reacting with Texas Red-labled antibody to K14, which stains the periderm, and fluorescein isothiocyanate-labeled antibody to K1 (Poly19056), which stains the upper layers of the epidermis, but not the periderm. Areas containing epitopes recognized by both K14 and the other antibody stain yellow or orange. Photo courtesy of DR Roop, Baylor College of Medicine. Reproduced from JR Bickenbach, JM Greer, DS Bundman, JA Rothangel, DR Roop. Loricrin expression is coordinated with other epidermal proteins and the appearance of lipid lamellar granules in development. J Invest Derm 10(3):405-410, 1995. Reprinted by permission of Blackwell Science Inc.
  • Purified_Keratin-1_Antibody_IHC_021715
    Double-label immunofluorescence analysis of developing mouse epidermis showing E18 epidermis reacting with Texas Red-labled antibody to K14, which stains the periderm, and fluorescein isothiocyanate-labeled antibody to K1 (Poly19056), which stains the upper layers of the epidermis, but not the periderm. Areas containing epitopes recognized by both K14 and the other antibody stain yellow or orange. Photo courtesy of DR Roop, Baylor College of Medicine. Reproduced from JR Bickenbach, JM Greer, DS Bundman, JA Rothangel, DR Roop. Loricrin expression is coordinated with other epidermal proteins and the appearance of lipid lamellar granules in development. J Invest Derm 10(3):405-410, 1995. Reprinted by permission of Blackwell Science Inc.
  • Purified_Keratin-1_Antibody_WB_021715
    Total lysates (15 µg protein) from Mouse skin (Positive control) and Raw264.7 (Low expression negative control) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:1000 (1 µg/ml) purified Keratin 1 antibody, clone Poly19056. Proteins were visualized using chemiluminescence detection by incubation with HRP Donkey anti-Rabbit secondary antibody (Cat. No. 406401, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • Purified_Keratin-2_Antibody_IHC_031521
    IHC staining of purified anti-Keratin 1 antibody (Poly19056) on formalin-fixed paraffin-embedded rat skin tissue. Following antigen retrieval using Citrate (Cat. No. 420901), the tissue was incubated with 1.0 µg/mL of the primary antibody 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, AEC, Cat. No. 929501) was used for detection followed by hemotoxylin counterstaining, according to the protocol provided. The image was captured with a 20X objective.
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905602 25 µL 89 CHF
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905601 100 µL 288 CHF
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Description

Keratin 1 belongs to the keratin family of proteins which are fibrous structural proteins. It is a type II cytokeratin that forms the major cytoskeleton in suprabasal keratinocytes along with heterodimer type I partner KRT10 form. KRT1 and KRT10 mutations lead to congenital epidermolytic ichthyosis characterized by skin erosions and hyperkeratosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This monospecific polyclonal antibody was raised against a peptide sequence derived from the C-terminus of the mouse keratin 1 protein.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
WB - Verified
IHC-F - Reported in the literature, not tested in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution of 1:500 is suggested. For Western blotting, the suggested use of this reagent is 1.0 µg per mL (1:1000 dilution). For immunocytochemistry, a dilution of 1:500 is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB), immunofluorescence (IF) and immunohistochemistry (IHC). This antibody has been successfully used in both frozen and paraffin embedded tissues.

*Predicted MW = 67 kD

This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Croyle MJ, et al. 2011. Development 138: 1675. (IHC, IF) PubMed
  2. Wirt SE, et al. 2010. J. Cell Biol. 191: 809. (IHC) PubMed
  3. Hu Y, et al. 2001. Nature. 410:710.
  4. Yuspa SH, et al. 1989. J Cell Biol. 109:1207.
  5. Roop DR, et al. 1984. J Biol Chem. 259:8037.
  6. Mailleux AA, et al. 2007. Dec Cell. 12:221. (IHC)
  7. IShitsuka Y, et al. 2013. J Invest Dermatol. 133:2566. (WB)
  8. Sengupta A, et al. 2010. PLoS One. 5:12249. (IHC) PubMed
  9. Suzuki K, et al. 2009. Development. 136:367. (IHC) PubMed
  10. Levy V, et al. 2007. FASEB J. 21:1358. (IHC) PubMed
  11. Osakarsson T, et al. 2006. Genes Dev. 20:2024. (IHC) PubMed
  12. Liang Y. 2011. Patholog Res Int. 2011:93674. (IHC) PubMed
  13. Sachs N, et al. 2012. PNAS. 109:21468. (IF)
  14. Vidal VP, et al. 2005. Curr Biol. 15:1340. (IHC)
Product Citations
  1. Sengupta A, et al. 2010. PLoS One. 5:e12249. PubMed
  2. Chen H, et al. 2023. Regen Biomater. 10:rbac086. PubMed
  3. Suzuki K, et al. 2009. Development. 136:367-372. PubMed
  4. Kashgari G, et al. 2020. Developmental Cell. 52(6):764-778. PubMed
  5. Levy V, et al. 2007. FASEB J. 21:1358-1366. PubMed
  6. Ackermann T, et al. 2019. Commun Biol. 2:208. PubMed
  7. Linn SC, et al. 2019. Exp Dermatol. 28:383. PubMed
  8. Oskarsson T, et al. 2006. Genes Dev. 20:2024-2029. PubMed
  9. Tchegnon E, et al. 2021. JCI Insight. 6:. PubMed
  10. Tripurani SK, et al. 2018. Mol Biol Cell. 29:2784. PubMed
  11. Xu KK, et al. 2020. Invest Ophthalmol Vis Sci. 61:30:00. PubMed
  12. Wirt S, et al. 2010. J Cell Biol. 191:809-825. PubMed
  13. Weingarten G, et al. 2019. FASEB J. 33:2241. PubMed
  14. Liang Y 2011. Patholog Res Int. 2011:936794. PubMed
  15. Kok LF, et al. 2020. J Invest Dermatol. 140:1459. PubMed
  16. Li H, Zhou L, Dai J, et al. 2017. J Cell Physiol. 10.1002/jcp.25924. PubMed
  17. Yu Q, et al. 2021. eLife. 10:00. PubMed
  18. Brosseau JP, et al. 2018. Nat Commun. 9:5014. PubMed
  19. Croyle M, et al. 2011. Development. 138:1675-1685. PubMed
  20. Kang S, et al. 2020. Stem Cell Reports. 14:34. PubMed
RRID
AB_2801250 (BioLegend Cat. No. 905602)
AB_2801250 (BioLegend Cat. No. 905601)

Antigen Details

Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
16678 View all products for this Gene ID
UniProt
View information about Keratin 1 on UniProt.org

Related FAQs

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Go To Top Version: 6    Revision Date: 05.13.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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