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Purified anti-Pax-2 Antibody (Previously Covance catalog# PRB-276P)

Pricing & Availability
Clone
Poly19010 (See other available formats)
Regulatory Status
RUO
Other Names
Paired box protein Pax-2, paired box homeotic gene 2, Opdc, optic disc coloboma
Previously
Covance Catalog# PRB-276P
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
1_Purified_Pax-2_Antibody_IHC_021315
Pax2 staining on normal human kidney tissue, demonstrating strong nuclear staining of epithelial cells in some renal tubules.
  • 1_Purified_Pax-2_Antibody_IHC_021315
    Pax2 staining on normal human kidney tissue, demonstrating strong nuclear staining of epithelial cells in some renal tubules.
  • 2_Poly19010_PURE_Pax-2_Antibody_2_WB_112817
    Western blot analysis of cell lysates from MCF-7 (low expression negative control) and Daudi (positive control) cells using Pax-2 rabbit primary antibody (Clone Poly19010,1:1000 dilution, 1 µg/ml) and HRP Donkey anti-rabbit secondary antibody (Cat. No. 406401, 1:3000 dilution). Direct-Blot™ HRP anti-GAPDH (Cat. No. 649203) was used as a loading control (1:1000 dilution).
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901002 25 µL 89 CHF
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901001 200 µL 370 CHF
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Description

Pax genes are expressed during embryogenesis in a temporally and spatially restricted manner in the developing neural tube. Pax-2 has been identified to be involved in kidney and optic nerve development. It belongs to the Pax group 2, including Pax-5 and Pax-8. Paired box (Pax) proteins are tissue specific transcription factors that contain a paired domain and typically a partial or complete homeodomain.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Mouse, Chicken, Frog, Zebrafish
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This Pax-2 antibody was generated against a recombinant protein containing 22kD of the Pax-2 sequence corresponding to amino acids 188-385.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
WB - Verified
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
• WB: 1-2 µg/ml (1:500-1:1000)
• Gel shifts: 1:10
• IHC: 1:100

Tissue Sections: Formalin-fixed, paraffin-embedded tissues, acetone or PFA fixed frozen sections
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Retrieva-All 1 (Cat. No. 927901).
Incubation: 20 minutes at room temperature
Staining Localization: nuclear

Application Notes

This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC), immunopurification and DNA band supershift experiments.

*Predicted MW = 44 kD
Positive Control Tissue: normal kidney or renal adenocarcinoma

This antibody recognizes Pax-2A and Pax-2B (two products of the Pax-2 gene). When linked to agarose beads, it has been used to immunoprecipitate DNA sequences recognized by Pax-2.


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Hammes A, et al. 2001. Cell. 106:319.
  2. Phelps DE, Dressler GR. 1996. J Biol Chem. 271:7978.
  3. Rothenpieler UW, Dressler GR. 1993. Development. 119:711
  4. Dressler GR, et al. 1993. Nature. 362:65
  5. Dressler GR, Douglass EC. 1992. Proc Natl Acad Sci USA. 89:1179
  6. Puschel AW, et al. 1992. Mech Dev. 38:197
  7. Riley BB, et al. 1999. Development. 126:5669. (IHC)
Product Citations
  1. Dauleh S, et al. 2016. PLoS One. 11: 0158997. PubMed
  2. Ealy M, et al. 2016. Proc Natl Acad Sci U S A. 113: 8508 - 8513. PubMed
  3. Jia S, et al. 2022. Differentiation. 123:42. PubMed
  4. Cheng K, et al. 2022. Sci Adv. 8:eabn8485. PubMed
  5. Li X, et al. 2023. Nat Neurosci. 26:891. PubMed
  6. Gupta N, et al. 2022. Sci Transl Med. 14:eabj4772. PubMed
  7. Miltner AM, et al. 2019. Int J Mol Sci. 20. PubMed
  8. Prunskaite-Hyyryläinen R, et al. 2016. Hum Mol Genet. 25: 1059 - 1073. PubMed
  9. Chang SH, et al. 2021. eLife. 10:00. PubMed
  10. Sun WR, et al. 2020. Hum Mol Genet. . PubMed
  11. Desgrange A, et al. 2017. Development. 144:4704. PubMed
  12. Cleal L, et al. 2021. Dis Model Mech. :14. PubMed
  13. Gong C, et al. 2021. Cell Reports. 34(12):108889. PubMed
  14. Brodie-Kommit J, et al. 2021. Science Advances. 7(11):. PubMed
  15. Hoshi M, et al. 2018. J Am Soc Nephrol. 29:775. PubMed
  16. Estermann MA, et al. 2022. Front Cell Dev Biol. 10:1042759. PubMed
  17. Chow T, et al. 2020. NPJ Regen Med. 5:7. PubMed
  18. McNeill H, Reginensi A 2016. J Am Soc Nephrol. 10.1681/ASN.2016060611. PubMed
  19. Taguchi A, et al. 2017. Cell Stem Cell. 21:730. PubMed
  20. Mattei C, et al. 2019. Front Cell Dev Biol. 7:25. PubMed
  21. Huang L, et al. 2016. Neoplasia. 18:71-81. PubMed
  22. Estermann MA, et al. 2020. Cell Rep. 31:107491. PubMed
  23. Bosze B, et al. 2020. J Neurosci. 40:1501. PubMed
  24. Patel A, et al. 2020. Development. 147:. PubMed
  25. Pushchina EV, et al. 2021. Int J Mol Sci. 22:. PubMed
  26. Lindstrm NO, et al. 2021. Developmental Cell. 56:2381. PubMed
  27. Sasaki K, et al. 2021. Cell Reports. 35(5):109075. PubMed
  28. Reginensi A, et al. 2016. Nat Commun. 7:12309. PubMed
  29. O'Sullivan ML, et al. 2017. Glia. 65:1697. PubMed
  30. Zhao F, et al. 2016. Sci Rep. 6:23037. PubMed
  31. Bernstein CS, et al. 2018. Dev Biol. 440:137. PubMed
  32. Kim E, et al. 2019. Sci Rep. 9:5025. PubMed
  33. Khelifi G, et al. 2022. Sci Rep. 12:20340. PubMed
  34. Tan Z, et al. 2018. Sci Rep. 8:16618. PubMed
  35. Muscat A, et al. 2016. Clin Cancer Res. 22: 3560 - 3570. PubMed
  36. Omotehara T, et al. 2020. Dev Dyn. 249:754. PubMed
  37. Conceição R, et al. 2019. Invest Ophthalmol Vis Sci. 60:4727. PubMed
  38. Lindström NO, et al. 2018. J Am Soc Nephrol. 29:825. PubMed
  39. Haque F, et al. 2017. Dev Biol. 427:121. PubMed
  40. C Tao, X Zhang, 2016. Cell Rep. 17:1832-1844. PubMed
  41. Ohnmacht J, et al. 2016. Development. 143: 1464 - 1474. PubMed
  42. Zhang H, et al. 2019. Dev Cell. 48:780. PubMed
  43. Tulloch AJ et al. 2019. J Comp Neurol. 527(18):2948-2972 . PubMed
  44. Tsujimoto H, et al. 2020. Cell Reports. 31(1):107476. PubMed
  45. Charlton–Perkins M, et al. 2019. Glia. 67:1401. PubMed
  46. Atsuta Y, Takahashi Y 2016. Development. 143: 3549 - 3559. PubMed
  47. Estermann MA, et al. 2021. Front Cell Dev Biol. 9:735203. PubMed
  48. Patel A, et al. 2020. Development. dev193649. PubMed
RRID
AB_2565001 (BioLegend Cat. No. 901002)
AB_2565001 (BioLegend Cat. No. 901001)

Antigen Details

Biology Area
Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
5076 View all products for this Gene ID
UniProt
View information about Pax-2 on UniProt.org
Go To Top Version: 6    Revision Date: 09.22.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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