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Biotin anti-mouse CD71 Antibody

Pricing & Availability
Clone
RI7217 (See other available formats)
Regulatory Status
RUO
Other Names
Transferrin receptor, T9
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RI7217_Biotin_CD71_Antibody_1_060117
C57BL/6 mouse bone marrow cells were stained with Ter-119 APC and biotinylated CD71 (clone RI7217, top) or biotinylated rat IgG2a, κ isotype control (bottom), followed by SAV-PE. Data shown was gated on total bone marrow cells.
  • RI7217_Biotin_CD71_Antibody_1_060117
    C57BL/6 mouse bone marrow cells were stained with Ter-119 APC and biotinylated CD71 (clone RI7217, top) or biotinylated rat IgG2a, κ isotype control (bottom), followed by SAV-PE. Data shown was gated on total bone marrow cells.
  • RI7217_Biotin_CD71_Antibody_2_060117
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113803 50 µg 67€
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Description

CD71 is a 95 kD type II heterodimeric transmembrane glycoprotein that is also known as T9 and transferrin receptor. CD71 is expressed on proliferating cells, reticulocytes, and erythroid precursors. Its expression is very low on resting leukocytes. CD71 plays a role in the control of cellular proliferation by facilitating the uptake of iron via ferrotransferrin binding and the recycling of apotransferrin to the cell surface.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: blocking of cellular proliferation. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 113821 & 113822).

This clone may also be known as R17217 or R17 217.

Application References

(PubMed link indicates BioLegend citation)
  1. Trowbridge I, et al. 1982. J. Cell. Physiol. 112:403.
  2. Grisendi S, et al. 2005. Nature 437:147.
  3. van Rooy I, et al. 2010. J. Control Release 150:30. PubMed
  4. Willhelm BT, et al. 2011. Blood 117:27. PubMed
  5. Okasi Y, et al. 2012. Exp Hematol. 40:143PubMed
  6. Stojanov K, et al. 2012. Mol Pharm. 9:1620. PubMed
Product Citations
  1. Liu Y, et al. 2022. Nat Commun. 13:1327. PubMed
  2. Bouthelier A, et al. 2023. iScience. 26:105739. PubMed
  3. Medina S, et al. 2023. Water (Basel). 15:. PubMed
  4. Nazarov K, et al. 2023. Int J Mol Sci. 24:. PubMed
  5. Tanimura N et al. 2018. Developmental cell. 46(5):581-594 . PubMed
  6. Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed
  7. Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed
  8. Gonzalez-Perez G, et al. 2016. J Immunol. 196: 3768 - 3779. PubMed
  9. Duek A, et al. 2014. Blood. 123:3943. PubMed
  10. Kubovcakova L, et al. 2013. Blood. 121:1188. PubMed
  11. Xu C, et al. 2022. Elife. 11:. PubMed
  12. Abramsson-Zetterberg L, et al. 2013. Mutat Res. 752:8. PubMed
RRID
AB_313564 (BioLegend Cat. No. 113803)

Antigen Details

Structure
Transferrin receptor and is 95 kD.
Distribution

Most dividing cells, the brain endothelium, and cycling hematopoietic progenitors.

Function
Mediates iron uptake.
Ligand/Receptor
Transferrin.
Cell Type
Hematopoietic stem and progenitors
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Hentze MW, et al. 1996. P. Natl. Acad. Sci. USA 93:8175.
2. Trowbridge IS, et al. 1993. Annu. Rev. Cell Biol. 9:129.
3. Trowbridge I, et al. 1982. J. Cell Physiol. 112:403.
4. Kuhn LC, et al. 1984. Cell 37:95.

Gene ID
22042 View all products for this Gene ID
UniProt
View information about CD71 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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