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| Some tandem dyes including PE/Dazzle™ 594, APC/Fire™ 750, PE/Cy7, PE/Cy5, PerCP/Cy5.5 and especially APC/Cy7, can be bound non-specifically by monocytes and macrophages. In flow cytometry assays, this can create confounding results that are difficult to interpret. To counter this, BioLegend has formulated a new and effective blocking reagent, True-Stain Monocyte Blocker™. This non-antibody based blocking solution reduces non-specific monocyte binding to fluorophores without affecting desired staining of monocytes. Now you can immuno-label monocytes with confidence, fully trusting your true staining results. |
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| PBMCs were stained with 5 μl/test of antibody in 100 μl of cells at 1x106 cells/ml. Fig. A-C are CD3 APC/Cy7 co-stained with CD14 BV421™ with no blocking solution (A), TruStain FcX™ blocker alone (B) or the True-Stain Monoctye Blocker™ alone (C). Fig 1D-F are CD56 PE/Cy5 co-stained with CD14 BV421™ with no blocking solution (D), TruStain FcX™ blocker alone (E) or the True-Stain Monoctye Blocker™ alone (F). Fig. 1G-I are CD19 PE/Dazzle™ 594 co-stained with CD14 BV421™ with no blocking solution (G), TruStain FcX™ blocker alone (H) or the True-Stain Monoctye Blocker™ alone (I). |
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| Monocyte Blocking Buffer |
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| True-Stain Monocyte Blocker™ |
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| If you’re working with myeloid cells, we’ve got several webpages with useful information on myeloid cell phenotyping, functions, and products! Learn more about monocytes, macrophages, and dendritic cells. |  |
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| BioLegend proudly sponsors the webinar "Cell Signaling in Cancer: New Targets, New Hope", where a panel of experts discuss how cancer cells communicate and manipulate signaling pathways, and how this communication can be intervened for new cancer therapeutics. Watch the webinar… |
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| We return to the Talkin’ Immunology Podcast with guest Lenny Teytelman to discuss his website, protocols.io, and the challenges of protocol reproducibility, journal paywalls, and more. Listen to the podcast… |
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