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Biotin anti-mouse CD51 Antibody

Pricing & Availability
Clone
RMV-7 (See other available formats)
Regulatory Status
RUO
Other Names
αV integrin, Vitronectin Receptor, Integrin αV chain, ITGAV
Isotype
Rat IgG1, κ
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Product Citations
publications
RMV-7
C57BL/6 bone marrow cells stained with RMV-7 PE
  • RMV-7
    C57BL/6 bone marrow cells stained with RMV-7 PE
Compare all formats
Cat # Size Price Save
104103 50 µg ¥11,570
104104 500 µg ¥47,170
Description

CD51 is a 140 kD protein, also known as αV integrin, vitronectin receptor, and integrin αV. It is a member of the integrin family, expressed on activated T cells, polymorphonuclear granulocytes, platelets, blastocysts, and osteoclasts. CD51 forms heterodimers by association with integrins β1, β3, β5 or β6; these complexes then act as receptors for multiple extracellular matrix proteins (ECM). The αv integrin heterodimers have varied functions in development, stimulation/activation and homeostasis. The primary ligands for CD51 complexes are fibronectin, fibrinogen, vitronectin, thrombspondin, von Willebrand factor, and CD31. The RMV-7 antibody has been reported to block binding of CD51 to vitronectin, fibronectin, and CD31 in some cell types, as well as blocking LAK cell cytotoxicity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse IL-2-activated killer (LAK) cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, and blocking1-3 of CD51 adhesion and LAK cell cytotoxicity. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104109 & 104110).

Application References

(PubMed link indicates BioLegend citation)
  1. Takahashi K, et al. 1990. J. Immunol. 145:4371. (IP Block)
  2. Narumiya S, et al. 1994. Int. Immunol. 6:139. (Block)
  3. Piali L, et al. 1995. J. Cell Biol. 130:451. (Block)
  4. Delpino MV, et al. 2012. J. Leukoc Biol 91:285. PubMed.
Product Citations
  1. Gadomski S, et al. 2022. Cell Stem Cell. 29:528. PubMed
  2. Ernst MPT, et al. 2023. Hemasphere. 7:e824. PubMed
  3. Zhao M, et al. 2019. Cell Rep. 26:652. PubMed
  4. Kenswil KJG, et al. 2021. Cell Stem Cell. 28(4):653-670.e11. PubMed
  5. Felker S, et al. 2022. JCI Insight. 7:. PubMed
  6. Mesnieres M, et al. 2021. Cell Reports. 36(8):109618. PubMed
  7. Lu F, et al. 2022. Nat Commun. 13:2362. PubMed
  8. Han Y, et al. 2019. J Clin Invest. 130:26. PubMed
RRID
AB_313072 (BioLegend Cat. No. 104103)
AB_313072 (BioLegend Cat. No. 104104)

Antigen Details

Structure
Integrin family, associates with integrins β1, β3, β5 or β6, 140 kD
Distribution

Activated T cells, polymorphonuclear granulocytes, blastocysts, osteoclasts

Function
Adhesion
Ligand/Receptor
Fibronectin, fibrinogen, vitronectin, thrombspondin, von Willebrand factor, and CD31
Cell Type
Granulocytes, Mesenchymal Stem Cells, Osteoclasts, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Maxfield SR, et al. 1989. J. Exp. Med. 169:2173.
3. Piali L, et al. 1995. J. Cell Biol. 130:451.

Gene ID
16410 View all products for this Gene ID
UniProt
View information about CD51 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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