Ultra-LEAF Purified anti-mouse CD51 Antibody, CD51, RMV-7

Clone: RMV-7 (See other available formats)
Regulatory Status: RUO
Other Names: αV integrin, Vitronectin Receptor, Integrin αV chain, ITGAV
Isotype: Rat IgG1, κ
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Product Citations: publications

C57BL/6 mouse bone marrow granulocytes stained with CD51 (clone RMV-7) purified (filled histogram) or rat IgG1, κ purified isotype ctrl (open histogram) followed by anti-rat IgG PE.

Cat #	Size	Price	Save
104109	100 µg	¥34,700
104110	1 mg	¥112,390

Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CD51 is a 140 kD protein, also known as α_V integrin, vitronectin receptor, and integrin α_V. It is a member of the integrin family, expressed on activated T cells, polymorphonuclear granulocytes, platelets, blastocysts, and osteoclasts. CD51 forms heterodimers by association with integrins β₁, β₃, β₅ or β₆; these complexes then act as receptors for multiple extracellular matrix proteins (ECM). The α_v integrin heterodimers have varied functions in development, stimulation/activation and homeostasis. The primary ligands for CD51 complexes are fibronectin, fibrinogen, vitronectin, thrombspondin, von Willebrand factor, and CD31. The RMV-7 antibody has been reported to block binding of CD51 to vitronectin, fibronectin, and CD31 in some cell types, as well as blocking LAK cell cytotoxicity.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: BALB/c mouse IL-2-activated killer (LAK) cells
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested
IP, Block - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation¹, and blocking^1-3 of CD51 adhesion and LAK cell cytotoxicity. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104109 & 104110).
RRID: AB_2819798 (BioLegend Cat. No. 104109)
AB_2819798 (BioLegend Cat. No. 104110)

Antigen Details

Structure: Integrin family, associates with integrins β₁, β₃, β₅ or β₆, 140 kD
Distribution: Activated T cells, polymorphonuclear granulocytes, blastocysts, osteoclasts
Function: Adhesion
Ligand/Receptor: Fibronectin, fibrinogen, vitronectin, thrombspondin, von Willebrand factor, and CD31
Cell Type: Granulocytes, Mesenchymal Stem Cells, Osteoclasts, T cells
Biology Area: Angiogenesis, Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Maxfield SR, et al. 1989. J. Exp. Med. 169:2173.
3. Piali L, et al. 1995. J. Cell Biol. 130:451.
Gene ID: 16410 View all products for this Gene ID
UniProt: View information about CD51 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Related FAQs

Do you guarantee that your antibodies are totally pathogen free?: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Does BioLegend test each Ultra-LEAF™ antibody by functional assay?: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?: No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All CD51 Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-mouse CD51	RMV-7	FC
PE anti-mouse CD51	RMV-7	FC
Purified anti-mouse CD51	RMV-7	FC,IP,Block
TotalSeq™-A1008 anti-mouse CD51	RMV-7	PG
Ultra-LEAF™ Purified anti-mouse CD51	RMV-7	FC,IP,Block
TotalSeq™-C1008 anti-mouse CD51	RMV-7	PG
TotalSeq™-B1008 anti-mouse CD51	RMV-7	PG

Certificate of Analysis

Go To Top Version: 1 Revision Date: 11/15/2019

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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