- Clone
- HI264 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- VII 70312
- Other Names
- PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein, Glycophorin A, MNS blood group, GYPA
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![HI264_BV421_CD235a_Antibody_042723 HI264_BV421_CD235a_Antibody_042723](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/HI264_BV421_CD235a_Antibody_042723.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human red blood cells were stained with anti-human CD235a (Glycophorin A) (clone HI264) Brilliant Violet 421™ (filled histogram) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
Cat # | Size | Price | Save |
---|---|---|---|
349131 | 25 tests | ¥42,460 | |
349132 | 100 tests | ¥89,540 |
CD235a (Glycophorin A) is member of the glycophorin A family. It is a type I sialoglycoprotein with a molecular weight of 10 kD, present in the cell membrane as a homodimer. Glycophorin A is expressed by erythroid precursors and erythrocytes. It carries the antigen determinants for the MNS blood groups and has been proposed to be an inhibitor of hemagglutination and hemolysis. Glycophorin A binds siglec 5, the erythrocyte binding antigen (EBA-175) of P. falciparum and some viruses, including influenza virus and hepatitis A virus.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
-
Application References
(PubMed link indicates BioLegend citation) -
- Mason D, et al. Eds. 2002. Leucocyte Typing VII:White Cell Differentiation Antigens. Oxford University Press. (FC)
- RRID
-
AB_2941527 (BioLegend Cat. No. 349131)
AB_2941527 (BioLegend Cat. No. 349132)
Antigen Details
- Structure
- Member of the glycophorin A family; type I protein with a molecular weight of 10 kD; present in the membrane as homodimer
- Distribution
-
Erythrocytes and erythroid precursors
- Function
- Possible inhibitor of hemagglutination and hemolysis; carries the antigen determinants for the MNS blood groups
- Ligand/Receptor
- Siglec 5, erythrocyte binding antigen (EBA-175) of P. falciparum, receptor for the influenza virus, hepatitis A virus
- Cell Type
- Erythrocytes
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Reid ME. 2009. Immunohematology 25:95.
2. Palacajornsuk P. 2006. Immunohematology 22:171.
3. Pasvol G. 2003. Trends Parasitol. 19:430.
4. Takakuwa Y. 2001. Curr. Opin. Hematol. 8:80. - Gene ID
- 2993 View all products for this Gene ID
- UniProt
- View information about CD235a on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
-
It is lot-specific. On average it ranges between 2-4.
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Pacific Blue™ anti-human CD235a (Glycophorin A)
Human red blood cells stained with anti-human CD235a (clone ... -
PE anti-human CD235a (Glycophorin A)
Human red blood cells stained with HI264 PE -
Purified anti-human CD235a (Glycophorin A)
Human red blood cells stained with purified HI264 conjugated... -
FITC anti-human CD235a (Glycophorin A)
Human red blood cells stained with HI264 FITC -
PerCP/Cyanine5.5 anti-human CD235a (Glycophorin A)
Human red blood cells were stained with anti-human CD235a (c... -
PE/Cyanine7 anti-human CD235a (Glycophorin A)
Human red blood cells were stained with anti-human CD235a (c... -
APC anti-human CD235a (Glycophorin A)
Human red blood cells were stained with anti-human CD235a (c... -
APC/Cyanine7 anti-human CD235a (Glycophorin A)
Human red blood cells were stained with anti-human CD235a (c... -
TotalSeq™-A0574 anti-human CD235a (Glycophorin A)
-
PE/Dazzle™ 594 anti-human CD235a (Glycophorin A)
Human red blood cells were stained with CD235a (Glycophorin ... -
TotalSeq™-C0574 anti-human CD235a (Glycophorin A)
-
Alexa Fluor® 700 anti-human CD235a (Glycophorin A)
Human red blood cells were stained with anti-human CD235a (c... -
TotalSeq™-D0574 anti-human CD235a (Glycophorin A)
-
Alexa Fluor® 647 anti-human CD235a (Glycophorin A)
Human red blood cells were stained with anti-human CD235a (G... -
Biotin anti-human CD235a (Glycophorin A)
Human red blood cells were stained with biotinylated anti-hu... -
Brilliant Violet 421™ anti-human CD235a (Glycophorin A)
Human red blood cells were stained with anti-human CD235a (G... -
Spark UV™ 387 anti-human CD235a (Glycophorin A)
Human red blood cells stained with anti-human CD235a (clone ... -
TotalSeq™-B0574 anti-human CD235a (Glycophorin A)
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