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Pacific Blue™ anti-human CD235a (Glycophorin A) Antibody

Pricing & Availability
Clone
HI264 (See other available formats)
Regulatory Status
RUO
Workshop
VII 70312
Other Names
PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein, Glycophorin A, MNS blood group, GYPA
Isotype
Mouse IgG2a, κ
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Product Citations
publications
HI264_PB_052312
Human red blood cells stained with anti-human CD235a (clone HI264) Pacific Blue™ (filled histogram) or mouse IgG2a, κ Pacific Blue™ isotype control (open histogram).
  • HI264_PB_052312
    Human red blood cells stained with anti-human CD235a (clone HI264) Pacific Blue™ (filled histogram) or mouse IgG2a, κ Pacific Blue™ isotype control (open histogram).
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Save
349107 25 µg ¥32,340
349108 100 µg ¥71,060
Description

CD235a (Glycophorin A) is member of the glycophorin A family. It is a type I sialoglycoprotein with a molecular weight of 10 kD, present in the cell membrane as a homodimer. Glycophorin A is expressed by erythroid precursors and erythrocytes.  It carries the antigen determinants for the MNS blood groups and has been proposed to be an inhibitor of hemagglutination and hemolysis. Glycophorin A binds siglec 5, the erythrocyte binding antigen (EBA-175) of P. falciparum and some viruses, including influenza virus and hepatitis A virus.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood.  It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Mason D, et al. Eds. 2002. Leucocyte Typing VII:White Cell Differentiation Antigens. Oxford University Press. (FC)
Product Citations
  1. Borrelli MR, et al. 2020. Stem Cells Transl Med. 1.339583333. PubMed
  2. Zhang Q, et al. 2021. Nat Commun. 12:4592. PubMed
  3. Cai L, et al. 2018. Stem Cells Transl Med. 7:87. PubMed
  4. Kenswil KJG, et al. 2021. Cell Stem Cell. 28(4):653-670.e11. PubMed
  5. Herland A, et al. 2020. Nat Biomed Eng. 421:4. PubMed
  6. Garrido-Martin EM, et al. 2020. J Immunother Cancer. 8:00. PubMed
  7. Phansalkar R, et al. 2021. Elife. 10:. PubMed
  8. Deleon NMD, et al. 2021. J Tissue Eng Regen Med. 15:1105. PubMed
  9. Krampitz G, et al. 2016. Proc Natl Acad Sci U S A. 113: 4464-4469. PubMed
  10. Sontheimer-Phelps A, et al. 2019. Cell Mol Gastroenterol Hepatol. 9:507. PubMed
  11. Chou DB, et al. 2020. Nat Biomed Eng. 0.440277778. PubMed
RRID
AB_11219199 (BioLegend Cat. No. 349107)
AB_11219199 (BioLegend Cat. No. 349108)

Antigen Details

Structure
Member of the glycophorin A family; type I protein with a molecular weight of 10 kD; present in the membrane as homodimer
Distribution

Erythrocytes and erythroid precursors

Function
Possible inhibitor of hemagglutination and hemolysis; carries the antigen determinants for the MNS blood groups
Ligand/Receptor
Siglec 5, erythrocyte binding antigen (EBA-175) of P. falciparum, receptor for the influenza virus, hepatitis A virus
Cell Type
Erythrocytes
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Reid ME. 2009. Immunohematology 25:95.
2. Palacajornsuk P. 2006. Immunohematology 22:171.
3. Pasvol G. 2003. Trends Parasitol. 19:430.
4. Takakuwa Y. 2001. Curr. Opin. Hematol. 8:80.

Gene ID
2993 View all products for this Gene ID
UniProt
View information about CD235a on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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