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Brilliant Violet 421™ anti-mouse CD41 Antibody

Pricing & Availability
Clone
MWReg30 (See other available formats)
Regulatory Status
RUO
Other Names
Fibrinogen receptor, gpIIb/IIIa, integrin alpha IIb, CD41a
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
MWREG30_BV421_112811
C57BL/6 platelets were stained with CD41 (clone MWReg30) Brilliant Violet 421™ (filled histogram) or rat IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
  • MWREG30_BV421_112811
    C57BL/6 platelets were stained with CD41 (clone MWReg30) Brilliant Violet 421™ (filled histogram) or rat IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Save
133911 125 µL ¥44,880
133912 50 µg ¥63,230
Description

CD41, also known as integrin α2b and GPIIb, is a transmembrane glycoprotein that is expressed by platelets and megakaryocytes. It was reported that CD41 is also expressed on hematopoietic progenitors. CD41 associates with CD61 (integrin β3) to form complexes that interact with fibrinogen, fibronectin, von Willebrand factor, and thrombin. CD41 is required for platelet adhesion and aggregation. Defect of CD41 leads to disorders of coagulation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse platelets
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: depletion of platelets and functional assay in vivo.4,7 The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies (Cat. No. 133939).

Application References

(PubMed link indicates BioLegend citation)
  1. Nieswandt B, et al. 1999. Blood 94:684.
  2. Teeling JL, et al. 2001. Blood 98:1095.
  3. Bertrand JY, et al. 2005. P. Natl. Acad. Sci. USA 102:134.
  4. Nocito A, et al. 2007. Hepatology 45:369. (Deplete)
  5. Sullivan BP, et al. 2010. Toxicol. Sci. 115:286. (Deplete) PubMed
  6. van der Heyde HC, et al. 2005. Blood 105:1956. (FA)
  7. Marjon KD, et al. 2009. J. Immunol. 182:1397. (Deplete)
Product Citations
  1. Sandovici I, et al. 2022. Dev Cell. 57:63. PubMed
  2. Li X, et al. 2022. Cell. 185:1709. PubMed
  3. Jordan KR, et al. 2022. J Thromb Haemost. 20:2035. PubMed
  4. Yamamoto R et al. 2018. Cell stem cell. 22(4):600-607 . PubMed
  5. Souilhol C, et al. 2016. Nat Commun. 7:10784. PubMed
  6. Yeung AK, et al. 2020. Blood Adv. 4:6204. PubMed
  7. Commerford CD et al. 2018. Cell reports. . PubMed
  8. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  9. Di Genua C, et al. 2020. Cancer Cell. 37(5):690-704.e8.. PubMed
  10. Delaney C, et al. 2020. Am J Physiol Lung Cell Mol Physiol. . PubMed
  11. Mizuno T, et al. 2017. Sci Rep. 7:42714. PubMed
  12. Marks-Bluth J, et al. 2015. Mol Cell Biol. 35:2165. PubMed
  13. Fujimoto N, et al. 2020. PLoS Biol. 18:e3000704. PubMed
  14. Davizon-Castillo P, et al. 2020. Physiol Rep. 8:e14386. PubMed
  15. Mesnieres M, et al. 2021. Cell Reports. 36(8):109618. PubMed
  16. Rao T, et al. 2015. Stem Cell Res. 14:307. PubMed
  17. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  18. Young K, et al. 2021. Cell Stem Cell. . PubMed
RRID
AB_10960744 (BioLegend Cat. No. 133911)
AB_10960744 (BioLegend Cat. No. 133912)

Antigen Details

Structure
A transmembrane glycoprotein that is expressed by platelets and megakaryocytes
Distribution

Platelets, megakaryocytes, and hematopoietic progenitors

Function
Associate with CD61 (integrin β3) to form a complex which plays a major role in platelet adhesion and aggregation
Ligand/Receptor
Fibrinogen, fibronectin, von Willebrand factor, thrombin
Cell Type
Hematopoietic stem and progenitors, Megakaryocytes, Platelets
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Bakewell SJ, et al. 2003. P. Natl. Acad. Sci. USA 100:14205.
2. Phillips DR, et al. 1991. Cell. 65:359.

Gene ID
16399 View all products for this Gene ID
UniProt
View information about CD41 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 2    Revision Date: 03/19/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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