- Clone
- Bu15 (See other available formats)
- Workshop
- V S143
- Other Names
- Integrin αx subunit, ITGAX, CR4, p150
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![Bu15_GMP_PerCP-Cyanine5-5_CD11c_081023 Bu15_GMP_PerCP-Cyanine5-5_CD11c_081023](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/Bu15_GMP_PerCP-Cyanine5-5_CD11c_081023.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Typical results from human peripheral blood monocytes stained either with Bu15 PerCP/Cyanine5.5 used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
CD11c is a 145-150 kD type I transmembrane glycoprotein also known as integrin αx and CR4. CD11c non-covalently associates with integrin β2 (CD18) and is expressed on monocytes/macrophages, dendritic cells, granulocytes, NK cells, and subsets of T and B cells. CD11c has been reported to play a role in adhesion and CTL killing through its interactions with fibrinogen, CD54, and iC3b.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 200.0 µg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
Clone Bu15 has a different binding epitope than clone 3.9. The binding of Bu15 with CD11c is divalent cation independent. Additional reported applications (for the relevant formats of this clone) include: inhibition of CD11c mediated adhesion and stimulation of chemokine production by monocytes.
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Application References
(PubMed link indicates BioLegend citation) -
- Sadhu C, et al. 2008. J. Immunoass. Immunoch. 29:42.
- Rezzonico R, et al. 2001. Blood 97:2932.
- Sadhu C, et al. 2007. J. Leukoc. Biol. 81:1395.
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Disclaimer
-
GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:
- Batch-to-batch consistency
- Material traceability
- Documented procedures
- Documented employee training
- Equipment maintenance and monitoring records
- Lot-specific certificates of analysis
- Quality audits per ISO 13485:2016
- QA review of released products
Antigen Details
- Structure
- Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 145-150 kD
- Distribution
-
Myeloid, dendritic cells, NK cells, B cells and T cell subsets
- Function
- Adhesion, CTL killing Ligand Receptor: CD54, fibrinogen, iC3b, ICAM-1, ICAM-4 Antigen
- Cell Type
- B cells, Dendritic cells, Neutrophils, NK cells, T cells
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Petty H. 1996. Immunol. Today 17:209.
2. Springer T. 1994. Cell 76:301.
3. Ihanus E, et al. 2007. Blood 109:802-810. - Gene ID
- 3687 View all products for this Gene ID
- UniProt
- View information about CD11c on UniProt.org
Other Formats
View All CD11c Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC/Cyanine7 anti-human CD11c
Human peripheral blood granulocytes stained with CD11c (clon... -
Purified anti-human CD11c
Human peripheral blood granulocytes stained with purified Bu... -
PE anti-human CD11c
Human peripheral blood lymphocytes stained with Bu15 PE -
APC anti-human CD11c
Human peripheral blood lymphocytes stained with Bu15 APC -
PerCP/Cyanine5.5 anti-human CD11c
Human peripheral blood granulocytes stained with Bu15 PerCP/... -
Pacific Blue™ anti-human CD11c
Human peripheral blood granulocytes stained with Bu15 Pacifi... -
FITC anti-human CD11c
Human peripheral blood monocytes stained with BU15 FITC. -
PE/Cyanine7 anti-human CD11c
Human peripheral blood monocytes stained with Bu15 PE/Cyanin... -
Alexa Fluor® 700 anti-human CD11c
Human peripheral blood monocytes were stained with CD11c (cl... -
Purified anti-human CD11c (Maxpar® Ready)
Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 162Dy-an... -
Brilliant Violet 421™ anti-human CD11c
Human peripheral blood monocytes were stained with CD11c (cl... -
PE/Dazzle™ 594 anti-human CD11c
Human peripheral blood lymphocytes were stained with CD11c (... -
Biotin anti-human CD11c
Human peripheral blood monocytes were stained with biotinyl... -
Alexa Fluor® 647 anti-human CD11c
Human peripheral blood monocytes were stained with CD11c (cl... -
PerCP anti-human CD11c
Human peripheral blood monocytes were stained with CD11c (cl... -
Alexa Fluor® 488 anti-human CD11c
Human peripheral blood monocytes were stained with CD11c (cl... -
Brilliant Violet 650™ anti-human CD11c
Human peripheral blood monocytes were stained with CD11c (cl... -
APC anti-human CD11c
Typical results from human peripheral blood lymphocytes stai... -
PE anti-human CD11c
Typical results from human peripheral blood lymphocytes stai... -
APC/Fire™ 750 anti-human CD11c
Human peripheral blood monocytes were stained with anti-huma... -
PE/Cyanine7 anti-human CD11c
Typical results from human peripheral blood monocytes staine... -
PerCP/Cyanine5.5 anti-human CD11c
Typical results from human peripheral blood monocytes staine... -
Pacific Blue™ anti-human CD11c
Typical results from human peripheral blood monocytes staine... -
GMP PE anti-human CD11c
Typical results from human peripheral blood lymphocytes stai... -
GMP APC anti-human CD11c
Typical results from human peripheral blood lymphocytes stai... -
Spark Blue™ 515 anti-human CD11c
Human peripheral blood cells were surface stained with (left... -
Spark YG™ 593 anti-human CD11c
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP Pacific Blue™ anti-human CD11c
Typical results from human peripheral blood monocytes staine... -
GMP PerCP/Cyanine5.5 anti-human CD11c
Typical results from human peripheral blood monocytes staine... -
PE/Fire™ 640 anti-human CD11c
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Fire™ 700 anti-human CD11c
Human peripheral blood cells were stained with anti-human CD... -
Brilliant Violet 711™ anti-human CD11c
Human peripheral blood lymphocytes were stained with anti-hu... -
Brilliant Violet 785™ anti-human CD11c
Human peripheral blood lymphocytes were stained with anti-hu... -
Brilliant Violet 605™ anti-human CD11c
Human peripheral blood lymphocytes were stained with anti-hu...
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