PE anti-human CD147 Antibody anti-CD147

Clone: HIM6 (See other available formats)
Regulatory Status: RUO
Workshop: VI N-L109
Other Names: Neurothelin, Basigin, EMMPRIN
Isotype: Mouse IgG1, κ
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HIM6_PE_CD147_Antibody_072718 — Human peripheral blood lymphocytes were stained with CD147 (clone HIM6) PE (filled histogram) or Mouse IgG1, κ PE isotype control (open histogram).

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Cat #	Size	Price	Save
306211	25 tests	¥25,740
306212	100 tests	¥57,640

Description

CD147, also known as neurothelin or basigin, is a member of the Ig superfamily. It is a 55-65 kD type I transmembrane glycoprotein which is primarily expressed on leukocytes, erythrocytes, platelets, and endothelial cells. CD147 is reported to have a function during embryonal brain development and/or play a role in integrin-mediated adhesion in brain endothelia.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: Chimpanzee, Baboon, Capuchin Monkey
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human PBMCs
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: inhibition of T cell activation², immunohistochemical staining^1,3 of frozen tissue sections and formalin-fixed paraffin-embedded tissue sections, and Western blotting¹. The Ultra-LEAF™ Purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 306221 and 306222).
RRID: AB_2750167 (BioLegend Cat. No. 306211)
AB_2750167 (BioLegend Cat. No. 306212)

Antigen Details

Structure: Ig superfamily, type I transmembrane glycoprotein, 55-65 kD
Distribution: Leukocytes, erythrocytes, platelets, endothelial cells
Function: Adhesion
Cell Type: Endothelial cells, Erythrocytes, Leukocytes, Platelets
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Biswas C, et al. 1995. Cancer Res. 55:434.
2. Fadool J, et al. 1993. Dev. Dyn. 196:252.
3. Felzmann T, et al. 1991. J. Clin. Immunol. 11:205.
Gene ID: 682 View all products for this Gene ID
UniProt: View information about CD147 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All CD147 Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-human CD147	HIM6	FC
Purified anti-human CD147	HIM6	FC,IHC-P
Alexa Fluor® 488 anti-human CD147	HIM6	FC,ICC
Alexa Fluor® 647 anti-human CD147	HIM6	FC,ICC,IHC-P
PE anti-human CD147	HIM6	FC
APC/Fire™ 750 anti-human CD147	HIM6	FC
APC anti-human CD147	HIM6	FC
PE/Cyanine7 anti-human CD147	HIM6	FC
PerCP/Cyanine5.5 anti-human CD147	HIM6	FC
Ultra-LEAF™ Purified anti-human CD147	HIM6	FC,WB,IHC,Block
TotalSeq™-C1137 anti-human CD147	HIM6	PG

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 07/27/2018

For Research Use Only. Not for diagnostic or therapeutic use.

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