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Purified anti-PP2AC Methyl (Leu 309) Antibody (Previously Covance catalog# MMS-5117)

Pricing & Availability
Clone
2A10 (See other available formats)
Regulatory Status
RUO
Other Names
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha, PPP2CA
Previously
Covance Catalog# MMS-5117
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
2A10_Purified_PP2AC_Antibody_WB_030915
N-terminally HA-tagged PPH21 was immunoprecipitated with 12CA5 anti-HA antibody (cross linked to protein A sepharose) from lysates of wtBY4741 (left) or PPM1δ (right) (lacking the PP2A methyltransferase) yeast cells, separated by 10% SDS-PAGE and blotted onto nitrocellulose membrane. Blots were incubated at 4°C o/n in a Miniblotter 45 (Immunetics) with 2A10 at the indicated dilutions. Anti-HA tag clone 16B12 served as positive control.
  • 2A10_Purified_PP2AC_Antibody_WB_030915
    N-terminally HA-tagged PPH21 was immunoprecipitated with 12CA5 anti-HA antibody (cross linked to protein A sepharose) from lysates of wtBY4741 (left) or PPM1δ (right) (lacking the PP2A methyltransferase) yeast cells, separated by 10% SDS-PAGE and blotted onto nitrocellulose membrane. Blots were incubated at 4°C o/n in a Miniblotter 45 (Immunetics) with 2A10 at the indicated dilutions. Anti-HA tag clone 16B12 served as positive control.
Cat # Size Price Save
828801 100 µL ¥73,700
This product is scheduled for discontinuation. Please use the availability checker to determine current availability.
Description

PP2AC (Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform), encoded by the PPP2CA gene, is the main phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2AC is reversibly methyl esterified on Leu-309 by leucine carboxyl methyltransferase 1 (LCMT1) and protein phosphatase methylesterase 1 (PPME1). This carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, which in turn, modulates the PP2A holoenzyme's enzyme activity and cellular localization.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Yeast
Reported Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a synthetic peptide from Human methylated Leucine 309 PP2A catalytic subunit.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
• WB: 1:100 - 1:800*
 

Application Notes

This antibody is effective in immunoblotting (WB).

* Expected MW: 36 kD

WB Positive Control: BY4741 yeast cells

The immunogen sequence (C-ßA-RRTPDYFL-Ome) is conserved from human, mouse to yeast.

Product Citations
  1. Padi SKR, et al. 2024. Nature. 195:625. PubMed
  2. Vit G, et al. 2022. EMBO J. 41:e110611. PubMed
RRID
AB_2564923 (BioLegend Cat. No. 828801)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Gene ID
5515 View all products for this Gene ID
UniProt
View information about PP2AC Methyl Leu309 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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