Ultra-LEAF™ Purified anti-human CD11b (activated) Antibody

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.

Endotoxin Level

Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IP, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The CBRM1/5 antibody recognizes a subset of CD11b molecules on neutrophils and monocytes activated with chemoattractants or phorbol-esters. This antibody does not recognize "non-activated" CD11b. The epitope recognized by CBRM1/5 is contained in the I domain of the a chain. Clone CBRM1/5 binds the I domain, close to the ligand binding site. It recognizes a conformational change of the integrin⁹.

Additional reported applications (for the relevant formats) include: immunoprecipitation, and blocking cell adhesion to fibrinogen and ICAM-1¹. The CBRM1/5 antibody binds specifically to the activated form of CD11b. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301425 and 301426).

Application References

(PubMed link indicates BioLegend citation)

1. Diamond M, et al. 1993. J. Cell Biol. 120:545. (Block)
2. Bryn T, et al. 2006. J. Immunol. 176:7361. PubMed
3. Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200.
4. Barthel SR,et al.2006. Am J. Respir Cell Mol Biol.35:378. PubMed
5. Pillay J,et al. 2010. J. Leukocyte Biol. 88:211. PubMed
6. Wilson RP, et al. 2011. Infect Immun. 79:830. PubMed
7. Koleva RI, et al. 2012. Blood. 119:4878. PubMed.
8. Eleftherious D, et al. 2012. Neurology. 79:2089. PubMed
9. Oxvig C, et al. 1999. Proc. Natl. Acad. Sci. USA 96:2215.
10. Muller-Edenborn B, et al. 2014. Br J Anaesth. PubMed
11. Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed

RRID

AB_2860762 (BioLegend Cat. No. 301425)
AB_2860762 (BioLegend Cat. No. 301426)

Antigen Details

Structure

Integrin, type I transmembrane glycoprotein, associates with integrin β₂ (CD18), 165-170 kD

Distribution

Granulocytes, monocytes/macrophages, dendritic cells, NK cells, T cell subset, B cell subset

Function

Adhesion, phagocytosis, chemotaxis, neutrophil activation

Ligand/Receptor

ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X

Cell Type

B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, NK cells, T cells, Tregs

Biology Area

Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers

Molecular Family

Adhesion Molecules, CD Molecules

Antigen References

1. Stewart M, et al. 1995. Curr. Opin. Cell Biol. 7:690.
2. Petty H, et al. 1996. Immunol. Today 17:209.

Gene ID

3684 View all products for this Gene ID

UniProt

View information about CD11b on UniProt.org

Documentation

• Technical data sheet
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

Certificate of Analysis