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Alexa Fluor® 488 anti-mouse/human CD11b Antibody

Pricing & Availability
Clone
M1/70 (See other available formats)
Regulatory Status
RUO
Other Names
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR, ITGAM
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
1_M1slash70_AF488_102110
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Alexa Fluor® 488 (filled histogram) or rat IgG2b Alexa Fluor® 488 isotype control (open histogram) (gated on total cell population).
  • 1_M1slash70_AF488_102110
    C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Alexa Fluor® 488 (filled histogram) or rat IgG2b Alexa Fluor® 488 isotype control (open histogram) (gated on total cell population).
  • 2_M1slash70_A488_CD11b_Antibody_IHCF_082117
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 2.5 µg/ml of CD11b (clone M1/70) Alexa Fluor® 488 (green), and co-stained with 5 µg/ml of CD3 (clone 17A2) Alexa Fluor® 647 (cyan) and 5 µg/ml of CD45R/B220 (clone RA3-6B2) Alexa Fluor® 594 (red) overnight at 4°C. The image was captured with a 10X objective.
  • 3_M170_A488_CD11b_Antibody_092021.png
    Paraformaldehyde-fixed (1%), 500 µm-thick mouse spleen section was processed according to the Ce3D™ Tissue Clearing Kit protocol (Cat. No. 427701). The section was costained with anti-mouse/human CD11b Antibody (clone M1/70) Alexa Fluor® 488 at 5 µg/mL (green), and anti-mouse IgD Antibody (clone 11-26c.2a) Alexa Fluor® 594 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
    Watch the video.
  • 4_52_Mouse_Liver_CD4_CD11c_CD11b
    Confocal image of C57BL/6 mouse liver sample acquired using the IBEX method of highly multiplexed antibody-based imaging: CD4 (yellow) in Cycle 1, CD11c (cyan) in Cycle 4, and CD11b (purple) in Cycle 4. Tissues were prepared using ~1% (vol/vol) formaldehyde and a detergent. Following fixation, samples are immersed in 30% (wt/vol) sucrose for cryoprotection. Images are courtesy of Drs. Andrea J. Radtke and Ronald N. Germain of the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
Compare all formats See Alexa Fluor® 488 spectral data
Cat # Size Price Save
101219 25 µg ¥17,980
101217 100 µg ¥41,300
Description

CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human, Cynomolgus, Rhesus
Reported Reactivity
Chimpanzee, Baboon, Rabbit
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/10 splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F, 3D IHC - Verified
SB - Community verified
SB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 2.5 - 10 µg per ml. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone M1/70 has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F).

Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1,4, in vitro blocking3,9,12, depletion2,8, immunofluorescence microscopy6,7,10, immunohistochemistry of acetone-fixed frozen sections5,11-13, and spatial biology (IBEX)35,36. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 101248).

Additional Product Notes

For use in spatial biology, this antibody has been demonstrated for use in immunohistochemistry using IBEX (Reported in the literature, not verified in house) and the NanoString GeoMx® Digital Spatial Profiler.

IBEX: Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

NanoString GeoMx®: This product has been verified for IHC-F (Immunohistochemistry - frozen tissue sections) and IHC-P (Immunohistochemistry - formalin-fixed paraffin-embedded tissues) on the NanoString GeoMx® Digital Spatial Profiler. The GeoMx® enables researchers to perform spatial analysis of protein and RNA targets in FFPE and fresh frozen human and mouse samples. For more information about our spatial biology products and the GeoMx® platform, please visit our spatial biology page.

Application References

(PubMed link indicates BioLegend citation)
  1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (IP)
  2. Ault K and Springer T. 1981. J. Immunol. 126:359. (Deplete)
  3. Springer TA, et al. 1982. Immunol. Rev. 68:171. (Block)
  4. Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766. (IP)
  5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
  6. Noel GJ, et al. 1990. J. Clin. Invest. 85:208. (IF)
  7. Allen LA and Aderem A. 1996. J. Exp. Med. 184:627 (IF)
  8. D'Amico A and Wu L. 2003. J. Exp. Med. 198:293. (Deplete)
  9. Brickson SJ, et al. 2003. Appl Physiol. 95:969. (Block)
  10. Clatworthy MR and Smith KG. 2004. J. Exp. Med. 199:717. (IF)
  11. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  12. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC)
  13. Iwasaki A and Kelsall BL. 2001. J. Immunol. 166:4884 (IHC, FC)
  14. Tailleux L. 2003. J. Exp. Med. 197:121. (Block, FC)
  15. Olver S, et al. 2006. Cancer Research 66:571. (FC)
  16. Tan SL, et al. 2006. J. Immunol. 176:2872. (FC) PubMed
  17. Ponomarev ED, et al. 2006. J. Immunol. 176:1402. (FC)
  18. Dzhagalov I, et al. 2007. Blood 109:1620. (FC)
  19. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  20. Rasmussen JW, et al. 2006. Infect. Immun.74:6590. PubMed
  21. Napimoga MH, et al. 2008. J. Immunol. 180:609. PubMed
  22. Elqaraz-Carmon V, et al. 2008. J. Lipid. Res. 49:1894. PubMed
  23. Kim DD, et al. 2008. Blood 112:1109. PubMed
  24. Guo Y, et al. 2008. Blood 112:480. PubMed
  25. Norian LA, et al. 2009. Cancer Res. 69:3086. (FC) PubMed
  26. Baumgartner CK, et al. 2010. J. Immunol. 184:573. PubMed
  27. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  28. Whiteland J, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
  29. Weber GF, et al. 2014. J Exp Med. 211:1243. PubMed
  30. Ashok A, et al. 2015. Toxicol Sci. 143:64. PubMed
  31. Price PJ, et al. 2015. J Immunol. 194:1164. PubMed
  32. Doni A, et al. 2015. J Exp Med. 212:905. PubMed
  33. Ferreira R, et al. 2016. J Infect Dis. 213: 669 - 673. PubMed
  34. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  35. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  36. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Vella JL, et al. 2021. Life Sci Alliance. 4:. PubMed
  2. Doughty D, et al. 2022. Exp Biol Med (Maywood). 247:1433. PubMed
  3. Madrid-Paulino E, et al. 2022. J Leukoc Biol. 112:475. PubMed
  4. Al-Saafeen BH, et al. 2023. Front Immunol. 13:1017780. PubMed
  5. Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed
  6. Barberio AE, et al. 2023. Bioeng Transl Med. 8:e10453. PubMed
  7. Kang YA, et al. 2023. J Exp Med. 220:. PubMed
  8. Shon WJ, et al. 2023. BMC Med. 21:165. PubMed
  9. Doloff JC, et al. 2023. Sci Adv. 9:eade9488. PubMed
  10. Laura C Burzynski et al. 2019. Immunity. 50(4):1033-1042 . PubMed
  11. Suzuki K, et al. 2019. Cell Mol Gastroenterol Hepatol. 8:119. PubMed
  12. Schwartz C, et al. 2014. J Immunol. 193:3590. PubMed
  13. Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed
  14. Wang Q, et al. 2022. Nat Commun. 13:3022. PubMed
  15. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  16. Ooi JD, et al. 2019. Nat Commun. 10:3392. PubMed
  17. Barberio AE, et al. 2020. ACS Nano. 14:11238. PubMed
  18. Yu X, et al. 2021. Nature. 594:560. PubMed
  19. Hickman SE, et al. 2019. Methods Mol Biol. 2034:305. PubMed
  20. Rangan P et al. 2019. Cell reports. 26(10):2704-2719 . PubMed
  21. Zhang H, et al. 2017. Leukemia. 10.1128/mBio.00226-17. PubMed
  22. Onishi S, et al. 2015. PLoS One. 10:126564. PubMed
  23. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  24. Sun P, et al. 2021. J Invest Dermatol. 141:1542. PubMed
  25. Hilpert C, et al. 2016. J Immunol. 197: 2780 - 2786. PubMed
  26. Jumabay M, et al. 2022. Sci Rep. 12:5614. PubMed
  27. Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
  28. Simonneau M, et al. 2018. Oncotarget. 9:36457. PubMed
  29. Chen C, et al. 2019. Cell Rep. 29:4200. PubMed
  30. Jiang H, et al. 2016. J Immunol. 197: 2880 - 2890. PubMed
  31. Pan RY, et al. 2019. Sci Adv. 5:eaau6328. PubMed
  32. Mooney C, et al. 2017. International Journal of Molecular Sciences. 10.3390/ijms18051037. PubMed
  33. Cheng Q, et al. 2020. Nat Nanotechnol. 0.842361111. PubMed
  34. Zhou L, et al. 2021. Transl Cancer Res. 10:5307. PubMed
  35. Ganguly K, et al. 2022. Front Immunol. 13:930449. PubMed
  36. Lin M, et al. 2019. Mol Med Rep. 19:1622. PubMed
  37. Cui H, et al. 2020. JCI Insight. 5:. PubMed
  38. Ullah I, et al. 2021. Immunity. 54:2143. PubMed
  39. Usui H, et al. 2007. Diabetes. 56:363. PubMed
  40. Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
  41. Venosa A, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L1487 - L1498. PubMed
  42. Rai MA, et al. 2020. Retrovirology. 17:35. PubMed
  43. França TT, et al. 2021. JCI Insight. 6:. PubMed
  44. Zhou L, et al. 2021. Transl Cancer Res. 10:3364. PubMed
  45. He J, et al. 2019. J Clin Invest. 130. PubMed
  46. Gong Y, et al. 2020. J Neuroinflammation. 0.845833333. PubMed
  47. Kar S, Colino J, Snapper C 2016. J Immunol. 196: 4204 - 4213. PubMed
  48. Li X, et al. 2020. Aging Dis. 0.821527778. PubMed
  49. Namiguchi K, et al. 2022. Front Cardiovasc Med. 8:761591. PubMed
  50. Huang L, et al. 2018. Exp Neurol. 300:41. PubMed
  51. Cheng HW, et al. 2022. Nat Commun. 13:2027. PubMed
  52. Cai B, et al. 2020. Cell Metabolism. 31(2):406-421.e7.. PubMed
  53. Miyajima M,et al. 2017. Nat Immunol.. 10.1038/ni.3867. PubMed
  54. Bochenek MA, et al. 2018. Nat Biomed Eng. 2:810. PubMed
  55. Berry MR et al. 2017. Cell. 170(5):860-874 . PubMed
  56. von Roemeling CA, et al. 2020. Nat Commun. 11:1508. PubMed
  57. Dymek B, et al. 2022. J Inflamm Res. 15:5621. PubMed
  58. Georgiadou A, et al. 2022. Elife. 11:. PubMed
  59. Sparber F, et al. 2019. Cell Host Microbe. 25:389. PubMed
  60. Wu X, et al. 2020. Front Immunol. 1.922222222. PubMed
  61. Roychoudhury J, et al. 2010. Invest Ophthalmol Vis Sci. 51:3560. PubMed
  62. Panka D, et al. 2013. Mol Cancer. 12:17. PubMed
  63. McAusland TM, et al. 2021. Mol Ther Oncolytics. 20:306. PubMed
  64. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  65. Garg BK, et al. 2019. PLoS One. 14:e0214942. PubMed
  66. Yamada K, et al. 2016. Cancer Res . 76: 4283 - 4292. PubMed
  67. Chmielewski M and Abken H 2017. Cell Rep.. 10.1016/j.celrep.2017.11.063. PubMed
  68. Stone S et al. 2018. Glia. 66(7):1331-1345 . PubMed
  69. Hsing CH, et al. 2021. Biomedicines. 9:. PubMed
  70. Chung EJ, et al. 2021. Int J Radiat Oncol Biol Phys. 110:526. PubMed
  71. Kim SH, et al. 2020. Neoplasia. 1.3375. PubMed
RRID
AB_389305 (BioLegend Cat. No. 101219)
AB_389305 (BioLegend Cat. No. 101217)

Antigen Details

Structure
Integrin family, associates with integrin β2 (CD18), 170 kD
Distribution

Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subsets of T and B cells

Function
Adhesion, chemotaxis
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Coxon A, et al. 1996. Immunity 5:653.

Gene ID
16409 View all products for this Gene ID 3684 View all products for this Gene ID
UniProt
View information about CD11b on UniProt.org

Related FAQs

If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?

It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.

Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?

Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.

Are other fluorophores compatible with IBEX?

Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.

The same antibody works in one tissue type but not another. What is happening?

Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.

How can I be sure the staining I’m seeing in my tissue is real?

In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.

Other Formats

View All CD11b Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse/human CD11b M1/70 FC
Biotin anti-mouse/human CD11b M1/70 FC
FITC anti-mouse/human CD11b M1/70 FC
PE anti-mouse/human CD11b M1/70 FC,IHC
PE/Cyanine5 anti-mouse/human CD11b M1/70 FC
Purified anti-mouse/human CD11b M1/70 FC,CyTOF®,IP,IHC-F,ICC
PE/Cyanine7 anti-mouse/human CD11b M1/70 FC
Alexa Fluor® 488 anti-mouse/human CD11b M1/70 FC,IHC-F,3D IHC,SB
Alexa Fluor® 647 anti-mouse/human CD11b M1/70 FC,3D IHC,SB
Alexa Fluor® 700 anti-mouse/human CD11b M1/70 FC
Pacific Blue™ anti-mouse/human CD11b M1/70 FC
APC/Cyanine7 anti-mouse/human CD11b M1/70 FC
PerCP/Cyanine5.5 anti-mouse/human CD11b M1/70 FC
PerCP anti-mouse/human CD11b M1/70 FC
Brilliant Violet 421™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 570™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 605™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 650™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 711™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 785™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 510™ anti-mouse/human CD11b M1/70 FC,ICC
Ultra-LEAF™ Purified anti-mouse/human CD11b M1/70 FC,CyTOF®,IP,Block,Depletion,IHC-F,ICC
Purified anti-mouse/human CD11b (Maxpar® Ready) M1/70 FC,CyTOF®
Alexa Fluor® 594 anti-mouse/human CD11b M1/70 IHC-F,FC
PE/Dazzle™ 594 anti-mouse/human CD11b M1/70 FC
APC/Fire™ 750 anti-mouse/human CD11b M1/70 FC
TotalSeq™-A0014 anti-mouse/human CD11b M1/70 PG
Brilliant Violet 750™ anti-mouse/human CD11b M1/70 FC
TotalSeq™-B0014 anti-mouse/human CD11b M1/70 PG
TotalSeq™-C0014 anti-mouse/human CD11b M1/70 PG
Spark NIR™ 685 anti-mouse/human CD11b M1/70 FC
PE/Fire™ 640 anti-mouse/human CD11b M1/70 FC
Spark YG™ 593 anti-mouse/human CD11b M1/70 FC
Spark YG™ 570 anti-mouse/human CD11b M1/70 IHC-F
PE/Fire™ 810 anti-mouse/human CD11b M1/70 FC
APC/Fire™ 810 anti-mouse/human CD11b Antibody M1/70 FC
Spark Blue™ 550 anti-mouse/human CD11b M1/70 FC
Spark UV™ 387 anti-mouse/human CD11b M1/70 FC
PerCP/Fire™ 806 anti-mouse/human CD11b M1/70 FC
PerCP/Fire™ 780 anti-mouse/human CD11b M1/70 FC
Spark Blue™ 574 anti-mouse/human CD11b (Flexi-Fluor™) M1/70 FC
KIRAVIA Blue 520™ anti-mouse/human CD11b M1/70 FC
PE/Fire™ 744 anti-mouse/human CD11b M1/70 FC
Spark PLUS UV395™ anti-mouse/human CD11b M1/70 FC
Spark Red™ 718 anti-mouse/human CD11b (Flexi-Fluor™) M1/70 FC
Go To Top Version: 5    Revision Date: 01/31/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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