APC/Cyanine7 anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
Ave. Rating
Submit a Review
Product Citations
publications
HK1-4_APCCyanine7_Ly-6C_Antibody_020119
C57BL/6 mouse bone marrow cells were stained with CD8a FITC and Ly-6C (clone HK1.4) APC/Cyanine7 (left) or Rat IgG2a, κ APC/Cyanine7 isotype control (right).
  • HK1-4_APCCyanine7_Ly-6C_Antibody_020119
    C57BL/6 mouse bone marrow cells were stained with CD8a FITC and Ly-6C (clone HK1.4) APC/Cyanine7 (left) or Rat IgG2a, κ APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
128025 25 µg 104€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
128026 100 µg 249€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Martin-Martin I, et al. 2022. Cell Rep. 39:110648. PubMed
  2. Tessaro FHG, et al. 2022. Cell Rep. 39:110977. PubMed
  3. Zhang X, et al. 2022. EBioMedicine. 84:104275. PubMed
  4. Hao J, et al. 2022. Cell Rep. 41:111804. PubMed
  5. Ullah I, et al. 2023. Cell Rep Med. 4:100893. PubMed
  6. Domingos-Pereira S, et al. 2022. Int J Mol Sci. 24: . PubMed
  7. Sieow JL, et al. 2023. Int J Mol Sci. 24: . PubMed
  8. Yang L, et al. 2023. Acta Pharmacol Sin. :. PubMed
  9. Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed
  10. Parsons M, et al. 2014. J Immunol. 192:1361. PubMed
  11. Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
  12. Zhang Q, et al. 2022. Nat Metab. 4:1055. PubMed
  13. Olofsen PA, et al. 2022. Cells. 11:. PubMed
  14. Zhao Q, et al. 2023. Signal Transduct Target Ther. 8:40. PubMed
  15. Tang C, et al. 2023. Nat Commun. 14:1493. PubMed
  16. Dijkman K, et al. 2023. NPJ Vaccines. 8:66. PubMed
  17. He J, et al. 2022. Front Immunol. 12:760138. PubMed
  18. Ippagunta S, et al. 2016. Proc Natl Acad Sci U S A. 113: E6162 - E6171. PubMed
  19. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
  20. Súarez-López L, et al. 2021. iScience. 24:103406. PubMed
  21. Sun CP, et al. 2021. PLoS Pathog. 17:e1009758. PubMed
  22. Abdala-Valencia H, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:482. PubMed
  23. Kim AS, et al. 2021. Cell. 184(17):4414-4429.e19. PubMed
  24. Heng Y, et al. 2020. Glia. . PubMed
  25. Chou YJ, et al. 2020. Sci Rep. 10:8422. PubMed
  26. Devalaraja S, et al. 2020. Cell. 1098:180. PubMed
  27. de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed
  28. Álvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed
  29. Tanner MA, et al. 2021. Am J Physiol Heart Circ Physiol. 321:H633. PubMed
  30. McDowell SAC, et al. 2021. Nat Cancer. 2:545. PubMed
  31. Zhou HF, et al. 2022. Front Pharmacol. 13:778755. PubMed
  32. Royzman D, et al. 2022. Front Immunol. 13:1012647. PubMed
  33. Lin T, et al. 2015. PLoS One. 10: e0138335. PubMed
  34. Wada N, et al. 2020. Mol Metab. 37:100988. PubMed
  35. Motoyama S, et al. 2020. Cells. :9. PubMed
  36. Chan YH, et al. 2019. EMBO Mol Med. 11:e10092. PubMed
  37. Walker FC, et al. 2021. PLoS Pathog. 17:e1009402. PubMed
  38. Movita D, et al. 2015. J Virol. 89:4809. PubMed
  39. Devalaraja S, et al. 2020. STAR Protoc. 1:100188. PubMed
  40. Hou X, et al. 2022. Cell Death Dis. 13:100. PubMed
  41. Fujita K, et al. 2019. Proc Natl Acad Sci U S A. 116:14714. PubMed
  42. Yu S, et al. 2016. Mol Ther. 10.1038/mt.2016.175. PubMed
  43. Yin S, et al. 2016. Nat Commun. 7: 11311. PubMed
  44. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  45. Wang Y, et al. 2021. Cell Rep. 35:108948. PubMed
  46. Formaglio P, et al. 2021. Immunity. 54:2724. PubMed
  47. Neupane AS, et al. 2020. Cell. 183(1):110-125.e11. PubMed
  48. Abdul Pari AA, et al. 2020. Cancer Res. 80:2586. PubMed
  49. Lim HY et al. 2018. Immunity. 49(2):326-341 . PubMed
  50. Guérin MV, et al. 2019. Nat Commun. 10:4131. PubMed
  51. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  52. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  53. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  54. Bambouskova M, et al. 2021. Cell Reports. 34(10):108756. PubMed
  55. AM C, et al. 2015. Proc Natl Acad Sci U S A. 112 7557 . PubMed
  56. Dmitrieva‐Posocco O et al. 2019. Immunity. 50(1):166-180 . PubMed
  57. Kuhn JA, et al. 2021. Elife. 10:. PubMed
  58. Lin J, et al. 2021. Nat Commun. 12:5321. PubMed
  59. Terauchi R, et al. 2021. PLoS One. 16:e0239108. PubMed
  60. Wang SK, et al. 2021. JCI Insight. 6: . PubMed
  61. Liu X, et al. 2020. Cell Host Microbe. 28(5):683-698.e6. PubMed
  62. Grauel AL, et al. 2020. Nat Commun. 4.84375. PubMed
  63. Lee L, et al. 2016. PLoS One. 11:e0167693. PubMed
  64. Maisel D, et al. 2016. PLoS One. 11: 0159716. PubMed
  65. Simões FC, et al. 2020. Nat Commun. 0.875. PubMed
  66. Luo J et al. 2018. Immunity. 49(1):107-119 . PubMed
  67. Shepardson K, et al. 2016. MBio. 7: e00506-16. PubMed
  68. Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
  69. Mossadegh–Keller N, et al. 2019. Bio Protoc. 9:5. PubMed
  70. Mohamed E, et al. 2020. Immunity. 52(4):668-682.e7.. PubMed
  71. Olson B, et al. 2021. Brain Behav Immun. 97:102. PubMed
  72. Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
  73. Capuccini B, et al. 2016. Sci Rep. 6:39258. PubMed
  74. de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed
  75. Walker MT, et al. 2021. Front. Allergy. 2:. PubMed
  76. Mulens-Arias V, et al. 2022. Pharmaceutics. 14:. PubMed
  77. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  78. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  79. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  80. Buerfent BC, et al. 2019. GMS Infect Dis. 7:Doc04. PubMed
  81. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  82. Fedele C, et al. 2021. J Exp Med. 218: . PubMed
  83. Nicolas-Boluda A, et al. 2021. eLife. 10:00. PubMed
  84. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  85. van de Garde M, et al. 2016. PLoS One. 11:e0166094. PubMed
  86. Baker GJ, et al. 2020. Cell Syst. 0.647222222. PubMed
  87. Gardner A, et al. 2022. J Immunother Cancer. 10:. PubMed
  88. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  89. Zhang X, et al. 2020. Glia. . PubMed
  90. Lynn GM, et al. 2020. Nat Biotechnol. 38:320. PubMed
RRID
AB_10643867 (BioLegend Cat. No. 128025)
AB_10643867 (BioLegend Cat. No. 128026)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org
Go To Top Version: 3    Revision Date: 02/01/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account