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Purified anti-mouse Ki-67 Antibody

Pricing & Availability
Clone
16A8 (See other available formats)
Regulatory Status
RUO
Other Names
KIA, proliferation-related Ki-67 antigen
Isotype
Rat IgG2a, κ
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Product Citations
publications
1_16A8_PURE_ki67_Antibody_FC_091918
Con A-stimulated (3 days) C57BL/6 mouse splenocytes were fixed and permeabilized with 70% ethanol, then stained with Ki-67 (clone 16A8) purified antibody (filled histogram) or rat IgG2a, κ isotype control (open histogram) followed by anti-rat IgG PE.
  • 1_16A8_PURE_ki67_Antibody_FC_091918
    Con A-stimulated (3 days) C57BL/6 mouse splenocytes were fixed and permeabilized with 70% ethanol, then stained with Ki-67 (clone 16A8) purified antibody (filled histogram) or rat IgG2a, κ isotype control (open histogram) followed by anti-rat IgG PE.
  • 2_16A8_PURE_ki67_Antibody_1_WB_082817_updated
    Total cell lysates (15 µg protein) from Raw264.7 and NIH3T3 were resolved by 3-8% Tris-Acetate gel electrophoresis, transferred to nitrocellulose, and probed with mouse Ki-67 antibody (clone 16A8). Proteins were visualized using a goat anti-rat IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin was used as a loading control.
  • 3_16A8_PURE_Ki-67_Antibody_ICC_051921.png
    TE‐71 cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X‐100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2.5 μg/mL of purified Ki‐67 (clone 16A8) in 5% FBS overnight at 4°C, followed by Alexa Fluor® 647 goat anti-rat IgG (clone Poly4054, red) for two hours. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
  • 4_16A8_PURE_Ki-67_Antibody_IHC-F_051921.png
    C57BL/6 mouse frozen intestine section was fixed with 4% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS plus 5% goat serum for 30 minutes at room temperature. Then the section was stained with 5 µg/mL purified Ki-67 (clone 16A8) in 5% FBS overnight at 4°C, followed by Alexa Fluor® 647 goat anti-rat IgG (clone Poly4054, red). Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
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652401 25 µg £61
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652402 100 µg £141
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Description

The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase, while in G0 phase the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli expressed partial mouse Ki-67 recombinant protein, 1816-2163 aa.
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

FC - Quality tested
WB, ICC, IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. For western blotting, suggested working dilution(s): Use 5 µl per 5 ml antibody dilution buffer for each mini-gel (0.5 µg/ml). For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 10.0 µg/mL is suggested. Additionally, each lot of this antibody is quality control tested by our Ki-67 staining protocol below. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Medina-Reyes EI, et al. 2015. Environ Res. 136:424. PubMed
  2. Guillaumond F, et al. 2015. PNAS. 112:2473. PubMed
  3. Sharma SK, et al. 2015. J Immunol. 194:5529. PubMed
  4. Rodero MP, et al. 2014. J. Invest. Dermatol. 7:1991-7. PubMed
Product Citations
  1. Arnold IC, et al. 2019. PLoS Pathog. 15:e1007866. PubMed
  2. Minamide K, et al. 2020. Sci Rep. 10:14639. PubMed
  3. Gouirand V, et al. 2022. EMBO J. 41:e110466. PubMed
  4. Bahreini F, et al. 2022. Bio Protoc. 12: . PubMed
  5. Mugarza E, et al. 2022. Sci Adv. 8:eabm8780. PubMed
  6. Stewart CE, et al. 2022. Cancers (Basel). 14: . PubMed
  7. Bahreini F, et al. 2022. Bio Protoc. 12:e4414. PubMed
  8. Hirose W, et al. 2022. Cell Mol Gastroenterol Hepatol. 15:153. PubMed
  9. Straß S, et al. 2023. Inflammopharmacology. 31:799. PubMed
  10. Herring CA et al. 2017. Cell systems. 6(1):37-51 . PubMed
  11. Xu L, et al. 2017. Int J Biol Macromol. . 10.1016/j.ijbiomac.2017.07.090. PubMed
  12. von Klitzing E, et al. 2018. Sci Rep. 20:594. PubMed
  13. Medina-Reyes E, et al. 2015. Environ Res. 136:424. PubMed
  14. Yuan CL, et al. 2018. Exp Ther Med. 9:1420. PubMed
  15. Heimesaat MM, et al. 2018. Eur J Microbiol Immunol (Bp). 1864:1609. PubMed
  16. Zingg D et al. 2018. Cancer cell. 34(1):69-84 . PubMed
  17. Mayer KA, et al. 2021. FASEB J. 35:e21217. PubMed
  18. Zappasodi R, et al. 2021. Nature. 591:652. PubMed
  19. Marable SS, et al. 2020. J Am Soc Nephrol. 2543:31. PubMed
  20. Brown EM, et al. 2019. Cell Host Microbe. 25:668. PubMed
  21. Yang SH, et al. 2017. Front Immunol. 8:1192. PubMed
  22. Matsumoto Y, et al. 2019. Sci Rep. 9:15244. PubMed
  23. Zhao L, et al. 2018. Nat Med. 24:1536. PubMed
  24. Javary J, et al. 2021. Liver Int. 41:1423. PubMed
  25. Hallam D, et al. 2015. Exp Gerontol. 67: 72-79. PubMed
  26. Hsiao CC, et al. 2021. Cells. 10:. PubMed
  27. Horiuchi S, et al. 2021. Sci Immunol. :eabm3131. PubMed
  28. Guillaumond F, et al. 2015. Proc Natl Acad Sci U S A. 112:2473. PubMed
  29. Wang C, et al. 2016. J Cell Biol. 212: 545-60. PubMed
  30. Hollinshead KER, et al. 2020. Cell Rep. 33:108231. PubMed
  31. Rore H, et al. 2021. Commun Biol. 4:802. PubMed
  32. Johnson SA, et al. 2021. Eur J Immunol. 51:3228. PubMed
  33. Villarreal-Ponce A, et al. 2020. Cell Rep. 33:108417. PubMed
  34. Clark K, et al. 2019. Front Immunol. 10:2355. PubMed
  35. Men L, et al. 2018. Cell Physiol Biochem. 51:2185. PubMed
  36. Heimesaat MM, et al. 2019. Front Immunol. 10:49. PubMed
  37. Lkhagvadorj K, et al. 2020. Am J Physiol Lung Cell Mol Physiol. 319:L742. PubMed
  38. Miller AJ, et al. 2020. Dev Cell. 117:53. PubMed
  39. Klose A, et al. 2018. JCSM Rapid Commun. 9:20222. PubMed
  40. Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed
  41. Wang G, et al. 2019. Front Immunol. 1.380555556. PubMed
  42. Stelekati E, et al. 2018. Cell Rep. 2.445833333. PubMed
  43. Matsumori T, et al. 2020. Cancer Res. 80:5305. PubMed
  44. Madouri F, et al. 2017. J Allergy Clin Immunol. 139:1650. PubMed
  45. Maier B, et al. 2020. Nature. 580:257. PubMed
  46. Hennchen M, et al. 2015. J Neurosci. 35: 16531 - 16544. PubMed
  47. Lee J, et al. 2019. Yonsei Med J. 60:667. PubMed
  48. Chen M, et al. 2018. Am J Physiol Endocrinol Metab. 315:E72. PubMed
RRID
AB_11204254 (BioLegend Cat. No. 652401)
AB_11204254 (BioLegend Cat. No. 652402)

Antigen Details

Structure
325 kD protein containing a forkhead-associated domain (FHA) and 13 tandem repeats
Distribution

Nucleus and chromosome

Function
Required for cell cycle progression and proliferation
Interaction
Interacts with KIF15; binds to MKI67IP through FHA domain
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Starborg M, et al. 1996. J. Cell. Sci. 109:143.
2. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
3. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
4. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
5. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
6. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.

Gene ID
17345 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
Ki-67
Specificity Alt (DOES NOT SHOW ON TDS):
Ki-67
App Abbreviation (DOES NOT SHOW ON TDS):
FC,WB,ICC,IHC-F
UniProt
View information about Ki-67 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 13    Revision Date: 02/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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