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DyLight™ 488 Donkey anti-rabbit IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4064 (See other available formats)
Regulatory Status
RUO
Isotype
Donkey Polyclonal Ig
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Product Citations
publications
Poly4064_D488_100410
293T cells were stained with anti-Tubulin-γ and secondarily labeled with DyLight™ 488 donkey anti-rabbit IgG (clone Poly4064).
  • Poly4064_D488_100410
    293T cells were stained with anti-Tubulin-γ and secondarily labeled with DyLight™ 488 donkey anti-rabbit IgG (clone Poly4064).
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406404 100 µg £73
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Description

This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rabbit
Antibody Type
Polyclonal
Host Species
Donkey
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with DyLight™ 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
FC - Reported in the literature, not verified in house
Recommended Usage

It is recommended that the reagent be titrated for optimal performance for each application.

* DyLight™ 488  has a maximum absorption of 493nm and a maximum emission of 518 nm (similar to Alexa Fluor® 488 or FITC).

Application Notes

This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.

Product Citations
  1. Aronovich A, et al. 2013. Blood. 122:2562. PubMed
  2. Zheng Q, et al. 2016. Sci Rep. 6: 33489. PubMed
  3. Hamsici S, et al. 2022. AIChE J. 68:. PubMed
  4. Palacios-Arreola M, et al. 2017. Sci Rep. 10.1038/s41598-017-10135-1. PubMed
  5. Parvatiyar K, et al. 2018. Nat Commun. 9:2770. PubMed
  6. Laugsch M, et al. 2019. Cell Stem Cell. 24:736. PubMed
  7. Hirano KI, et al. 2021. eLife. 0.416666666666667. PubMed
  8. Suzuki T, et al. 2020. J Clin Invest. 130:799. PubMed
  9. Flommersfeld S, et al. 2021. Immunity. :. PubMed
  10. Liu R, et al. 2017. PLoS One. 10.1371/journal.pone.0170666. PubMed
  11. Frank E, et al. 2016. Toxicol Pathol. 10.1177/0192623315620587. PubMed
  12. Du M, et al. 2022. Sci Adv. 8:eabj1570. PubMed
  13. Dudek M, et al. 2021. Nature. 592:444. PubMed
  14. Chen YC, et al. 2021. Antioxidants (Basel). 10:. PubMed
  15. Beam J, et al. 2015. J Biol Chem. 290: 23371-23384. PubMed
  16. Tsukamoto S, et al. 2021. Stem Cell Research. 53:102363. PubMed
  17. Chen Y, et al. 2014. Blood. 124:936. PubMed
  18. Kurashima Y, et al. 2021. Nat Commun. 12:1067. PubMed
  19. Chapman N, et al. 2013. J Immunol. 191:6208. PubMed
  20. Granato A, et al. 2014. J Immunol. 192:5761. PubMed
RRID
AB_1575130 (BioLegend Cat. No. 406404)

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Go To Top Version: 4    Revision Date: 01/06/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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