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Purified anti-p97/VCP Antibody

Pricing & Availability
Clone
4G9 (See other available formats)
Regulatory Status
RUO
Other Names
Transitional endoplasmic reticulum ATPase, p97, TERA
Isotype
Mouse IgG1, κ
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Product Citations
publications
4G9_WB_040910
Western blot analysis of extracts from Hela cells (Lane 1), Jurkat cells (Lane 2), MCF7 cells (Lane 3), or mouse splenocytes (Lane 4), using anti-p97/VCP, clone 4G9
  • 4G9_WB_040910
    Western blot analysis of extracts from Hela cells (Lane 1), Jurkat cells (Lane 2), MCF7 cells (Lane 3), or mouse splenocytes (Lane 4), using anti-p97/VCP, clone 4G9
Cat # Size Price Quantity Check Availability Save
636801 25 µg £77
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636802 100 µg £157
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Description

p97/VCP (valosin containing protein) belongs to the AAA ATPase family, predicted molecular weight 97 kD. VCP is associated with a variety of cellular activities, including cell cycle control, membrane fusion after mitosis, regulates spindle disassembly at the end of mitosis, nuclear trafficking and the ubiquitin-proteasome degradation pathway. In many instances, specific adaptor proteins with which VCP forms multiprotein complexes direct its role in a given process. This protein is required for the fusion of ER and Golgi membranes. Clone 4G9 has been shown to be useful for western blotting, immunoprecipitation, and immunofluorescence staining of human p97/VCP protein.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human valosin containing protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP, ICC - Reported by the developer, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 µg per 5 mL antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1~4 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Shchedrina VA, et al. 2011. J Biol Chem. 186:42937. PubMed.
  2. Yi L, et al. 2012. Hum Mol Genet. 21:1794. PubMed.
  3. Yahiro K, et al. 2012. Infect Immun. 80:1803. PubMed.
Product Citations
  1. Yi L, et al. 2012. Hum Mol Genet. 2.120833333. PubMed
  2. Yahiro K, et al. 2012. Infect Immun. 80:1803. PubMed
  3. Efimova I, et al. 2021. Front Immunol. 12:643144. PubMed
  4. Shchedrina V, et al. 2011. J Biol Chem. 286:42937. PubMed
RRID
AB_2214641 (BioLegend Cat. No. 636801)
AB_2214641 (BioLegend Cat. No. 636802)

Antigen Details

Structure
Belongs to the AAA ATPase family, homohexamer, forms a ring-shaped particle of 12.5 nm diameter and displays 6-fold radial symmetry.
Interaction
VCIP135, SELS/VIMP, SYVN1, DERL1, DERL2, DERL3, SVIP
Bioactivity
VCP is associated with a variety of cellular activities, including cell cycle control, membrane fusion after mitosis, regulates spindle disassembly at the end of mitosis, nuclear trafficking and the ubiquitin-proteasome degradation pathway.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Neuroscience, Neuroscience Cell Markers
Molecular Family
Endoplasmic Reticulum Markers
Antigen References

1. Ye Y, et al. 2001. Nature 414:652.

Gene ID
7415 View all products for this Gene ID
UniProt
View information about p97/VCP on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 01/14/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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