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Alexa Fluor® 700 anti-human CD3 Antibody

Pricing & Availability
Clone
HIT3a (See other available formats)
Regulatory Status
RUO
Workshop
V CD03.05
Other Names
T3, CD3ε
Isotype
Mouse IgG2a, κ
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Product Citations
publications
HIT3a_Alx700_080408.jpg
Human peripheral blood lymphocytes stained with HIT3a Alexa Fluor® 700
  • HIT3a_Alx700_080408.jpg
    Human peripheral blood lymphocytes stained with HIT3a Alexa Fluor® 700
Compare all formats See Alexa Fluor® 700 spectral data
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300323 25 µg $124
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300324 100 µg $265
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation of T lymphocytes4-7. The HIT3a antibody is able to stimulate T cell activation. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300314). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  4. Sedelies KA, et al. 2004. J. Biol. Chem. 279:26581. (Activ)
  5. Rivollier A, et al. 2004. Blood 104:4029. (Activ)
  6. Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Activ)
  7. Smeltz RB. 2007. J. Immunol. 178:4786. (Activ)
Product Citations
  1. Kerns JS, et al. 2023. Bio Protoc. 13: . PubMed
  2. Masuda H, et al. 2014. J Am Heart Assoc. 3:743. PubMed
  3. Meng S, et al. 2018. Mol Med Rep. 18:4247. PubMed
  4. Tardif V, et al. 2019. Nat Commun. 10:823. PubMed
  5. Cho A, et al. 2019. Cell Rep. 28:909. PubMed
  6. Khanam A, et al. 2021. Front Immunol. 11:599648. PubMed
  7. Sinha D, et al. 2021. J Immunother Cancer. 9:. PubMed
  8. Amanat F, et al. 2021. Cell. 184:3936. PubMed
  9. Yin Q, et al. 2021. Proc Natl Acad Sci U S A. 118: . PubMed
  10. Obradovic A, et al. 2021. Cell. 184(11):2988-3005.e16. PubMed
  11. Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed
  12. Mudd PA, et al. 2022. Cell. 185:603. PubMed
  13. Scherpenisse M, et al. 2021. MBio. 12:. PubMed
  14. Egolf S, et al. 2021. Sci Adv. 7:eabj9141. PubMed
  15. Strijker JGM, et al. 2021. J Pers Med. 11:. PubMed
  16. O'Connor MH, et al. 2021. Commun Biol. 4:563. PubMed
  17. Clayton KL, et al. 2021. Cell Host Microbe. 29(3):435-447.e9. PubMed
  18. Dusseaux M, et al. 2011. Blood. 117:1250. PubMed
  19. Todnem Sakkestad S, et al. 2019. Pathogens. 8. PubMed
  20. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  21. Connors J, et al. 2022. Geroscience. :. PubMed
RRID
AB_493738 (BioLegend Cat. No. 300323)
AB_493738 (BioLegend Cat. No. 300324)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK-T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD3
Specificity Alt (DOES NOT SHOW ON TDS):
CD3
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD3 on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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