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Alexa Fluor® 700 anti-human CD8 Antibody

Pricing & Availability
Clone
SK1 (See other available formats)
Regulatory Status
RUO
Other Names
T8, Leu2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
SK1_AF700_CD8_Antibody_FC_111213.jpg
Human peripheral blood lymphocytes were stained with CD8 (clone SK1) Alexa Fluor® 700 (filled histogram) or mouse IgG1, κ Alexa Fluor® 700 isotype control (open histogram).
  • SK1_AF700_CD8_Antibody_FC_111213.jpg
    Human peripheral blood lymphocytes were stained with CD8 (clone SK1) Alexa Fluor® 700 (filled histogram) or mouse IgG1, κ Alexa Fluor® 700 isotype control (open histogram).
Compare all formats See Alexa Fluor® 700 spectral data
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344723 25 tests $112
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344724 100 tests $276
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Description

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Chimpanzee, Pigtailed Macaque, Sooty Mangabey
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics8, immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX)9,10. This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter JA, et al. 1981. J. Exp. Med. 153:310.
  2. Campanelli R, et al. 2002. Intl. Immunol. 14:39.
  3. Evans RL, et al. 1981. Immunol. 78:544.
  4. Wooldridge L, et al. 2005. J. Bio. Chem. 280:27491.
  5. Ch'el IL, et al. 2011. J Exp Med. 208:633. PubMed
  6. Carbone A, et al. 1999. Blood 93:2319. (IHC-F)
  7. Ahmed A, et al. 2001. J. Pathol. 193:383. (IHC)
  8. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  9. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  10. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Jaiswal A, et al. 2022. Cancer Cell. 40:524. PubMed
  2. Charpentier JC, et al. 2020. Nat Commun. 11:180. PubMed
  3. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
  4. Vyasamneni R, et al. 2023. Cell Rep Methods. 3:100388. PubMed
  5. Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed
  6. Crespo ÂC, et al. 2020. Cell. 182(5):1125-1139. PubMed
  7. Yarzabek B et al. 2018. eLife. 7 pii: e34961. PubMed
  8. Pellett Madan R, et al. 2020. Clin Transplant. 34:e14021. PubMed
  9. Neidleman J, et al. 2020. Cell Rep Med. 100081:1. PubMed
  10. Ma X, et al. 2021. Cell Metabolism. 33(5):1001-1012.e5. PubMed
  11. Ackerley CG, et al. 2022. Front Immunol. 13:972170. PubMed
  12. Ott PA, et al. 2020. Cell. 183(2):347-362.e24. PubMed
  13. Serra EG, et al. 2020. Nat Commun. 11:995. PubMed
  14. Williamson LM, et al. 2021. NPJ Precis Oncol. 5:103. PubMed
  15. Vanoni G, et al. 2022. Bio Protoc. 218:. PubMed
  16. Cohen CA, et al. 2021. Nat Commun. 12:4678. PubMed
  17. Coufal S, et al. 2019. Cells. 8. PubMed
  18. Salvany‐Celades M et al. 2019. Cell Rep. 27(9):2537-2547 . PubMed
  19. Bradley D, et al. 2022. Nat Commun. 13:5606. PubMed
  20. FitzPatrick MEB, et al. 2021. Cell Rep. 34:108661. PubMed
  21. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  22. Zhong W, et al. 2022. Front Immunol. 13:1001255. PubMed
RRID
AB_2562789 (BioLegend Cat. No. 344723)
AB_2562789 (BioLegend Cat. No. 344724)

Antigen Details

Structure
Ig superfamily, homodimer or heterodimer with CD8b, 32-34 kD
Distribution

Majority of thymocytes, T cell subset, NK cells

Function
MHC class I co-receptor, thymic differentiation, T cell activation
Ligand/Receptor
MHC Class I molecules
Cell Type
NK cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.

Gene ID
925 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD8
Specificity Alt (DOES NOT SHOW ON TDS):
CD8
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD8 on UniProt.org

Other Formats

View All CD8 Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 647 anti-human CD8 SK1 FC
Brilliant Violet 650™ anti-human CD8 SK1 FC
Purified anti-human CD8 SK1 FC,IHC-F
FITC anti-human CD8 SK1 FC
PE anti-human CD8 SK1 FC
PerCP anti-human CD8 SK1 FC
PerCP/Cyanine5.5 anti-human CD8 SK1 FC
PE/Cyanine7 anti-human CD8 SK1 FC
APC/Cyanine7 anti-human CD8 SK1 FC
Alexa Fluor® 488 anti-human CD8 SK1 FC,SB
Pacific Blue™ anti-human CD8 SK1 FC
Biotin anti-human CD8 SK1 FC
APC anti-human CD8 SK1 FC
Alexa Fluor® 700 anti-human CD8 SK1 FC
Purified anti-human CD8 (Maxpar® Ready) SK1 FC,CyTOF®
Brilliant Violet 510™ anti-human CD8 SK1 FC
Brilliant Violet 711™ anti-human CD8 SK1 FC
Brilliant Violet 785™ anti-human CD8 SK1 FC
Brilliant Violet 605™ anti-human CD8 SK1 FC
PE/Dazzle™ 594 anti-human CD8 SK1 FC
PE anti-human CD8 SK1 FC
APC/Fire™ 750 anti-human CD8 SK1 FC
APC anti-human CD8 SK1 FC
Brilliant Violet 421™ anti-human CD8 SK1 FC
Pacific Blue™ anti-human CD8 SK1 FC
TotalSeq™-A0046 anti-human CD8 SK1 PG
TotalSeq™-C0046 anti-human CD8 SK1 PG
Brilliant Violet 750™ anti-human CD8 SK1 FC
TotalSeq™-B0046 anti-human CD8 SK1 PG
Spark Blue™ 550 anti-human CD8 SK1 FC
PE/Cyanine7 anti-human CD8 SK1 FC
FITC anti-human CD8 SK1 FC
APC/Fire™ 810 anti-human CD8 SK1 FC
PE/Fire™ 640 anti-human CD8 SK1 FC
PE/Fire™ 700 anti-human CD8 SK1 FC
PerCP anti-human CD8 SK1 FC
PerCP/Cyanine5.5 anti-human CD8 SK1 FC
TotalSeq™-D0046 anti-human CD8 SK1 PG
APC/Fire™ 750 anti-human CD8 SK1 FC
GMP APC anti-human CD8 SK1 FC
PE/Cyanine5 anti-human CD8 Antibody SK1 FC
Spark UV™ 387 anti-human CD8 SK1 FC
GMP PE anti-human CD8 SK1 FC
GMP PE/Cyanine7 anti-human CD8 SK1 FC
Spark NIR™ 685 anti-human CD8 SK1 FC
KIRAVIA Blue 520™ anti-human CD8 SK1 FC
GMP FITC anti-human CD8 SK1 FC
GMP Pacific Blue™ anti-human CD8 SK1 FC
GMP PerCP anti-human CD8 SK1 FC
Spark Violet™ 500 anti-human CD8 SK1 FC
GMP APC/Fire™ 750 anti-human CD8 SK1 FC
GMP PerCP/Cyanine 5.5 anti-human CD8 SK1 FC
Alexa Fluor® 660 anti-human CD8a SK1 FC
Spark Blue™ 515 anti-human CD8 SK1 FC
Spark Blue™ 574 anti-human CD8 SK1 FC
Spark Violet™ 538 anti-human CD8 SK1 FC
PE/Fire™ 810 anti-human CD8 SK1 FC
Spark YG™ 593 anti-human CD8 SK1 FC
Go To Top Version: 4    Revision Date: 07/13/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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