Loading...

PE anti-mouse I-A/I-E Antibody

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
M5/114.15.2
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) PE (solid line) or rat IgG2b, κ PE isotype control (dashed line).
  • M5/114.15.2
    C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) PE (solid line) or rat IgG2b, κ PE isotype control (dashed line).
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
107607 50 µg $92
Check Availability


Need larger quantities of this item?
Request Bulk Quote
107608 200 µg $329
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  17. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  18. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Liu T, et al. 2023. Cardiovasc Res. 119:1046. PubMed
  2. Sia ZR, et al. 2022. J Virol. 96:e0100622. PubMed
  3. Swan SL, et al. 2023. Front Immunol. 14:1085547. PubMed
  4. Matsuzawa M, et al. 2023. Nat Commun. 14:1417. PubMed
  5. Ji Y, et al. 2023. Nat Cell Biol. 25:726. PubMed
  6. Nishi W, et al. 2023. Nat Commun. 14:3157. PubMed
  7. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  8. Lu R, et al. 2022. Scand J Immunol. 96:e13177. PubMed
  9. Ostendorf BN, et al. 2022. Nature. 611:346. PubMed
  10. Yi M, et al. 2022. J Immunother Cancer. 10: . PubMed
  11. Ivasko SM, et al. 2023. Front Immunol. 13:1023206. PubMed
  12. Wang C, et al. 2023. Exp Mol Med. 55:347. PubMed
  13. Daemen S, et al. 2021. Cell Reports. 34(2):108626. PubMed
  14. Kisielow J, et al. 2019. Nat Immunol. 1.286111111. PubMed
  15. Li Q, et al. 2022. Cell Rep. 40:111308. PubMed
  16. Platt DJ, et al. 2021. Cell Reports. 35(6):109113. PubMed
  17. Shi L, et al. 2019. Theranostics. 9:4115. PubMed
  18. Espinosa-Cueto P, et al. 2017. PLoS One.. 10.1371/journal.pone.0182126. PubMed
  19. Liang S, et al. 2022. Acta Pharm Sin B. 12:2494. PubMed
  20. Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed
  21. Liu W, et al. 2021. Cell Mol Immunol. 18:2660. PubMed
  22. Zhu J, et al. 2016. Sci Rep. 6:27136. PubMed
  23. Yasmin N, et al. 2013. J Exp Med. 210:2597. PubMed
  24. Blumenthal D, et al. 2020. eLife. 9:e55995.. PubMed
  25. Cai W, et al. 2020. J Immunol Res. 2019:2835256. PubMed
  26. Gómez-Díaz C, et al. 2021. iScience. 24:103241. PubMed
  27. Christopher S Garris et al. 2018. Immunity. 49(6):1148-1161 . PubMed
  28. Liu Y, et al. 2022. Nat Commun. 13:2665. PubMed
  29. Gordon RA, et al. 2020. PLoS One. 15:e0226396. PubMed
  30. Jiang S, et al. 2020. Scand J Immunol. e12867:91. PubMed
  31. Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed
  32. Han C, et al. 2021. Cell Reports. 34(6):108706. PubMed
  33. Yi M, et al. 2021. J Hematol Oncol. 14:27. PubMed
  34. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
  35. Dhandapani R, et al. 2018. Nat Commun. 9:1640. PubMed
  36. Tu J, et al. 2022. Theranostics. 12:747. PubMed
  37. Dumas AA, et al. 2020. EMBO J. 39:e103790. PubMed
  38. Liang Z, et al. 2020. Pain. 161:2089. PubMed
  39. Lin SY, et al. 2017. Autophagy. 14:778. PubMed
  40. Smith AS, et al. 2022. J Immunother Cancer. 10:. PubMed
  41. Zhao H, et al. 2021. Int J Nanomedicine. 16:4913. PubMed
  42. Tuganbaev T, et al. 2020. Cell. 182(6):1441-1459.e21. PubMed
  43. Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed
  44. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  45. Du Y, et al. 2022. Nat Commun. 13:231. PubMed
  46. Novkovic M, et al. 2016. PLoS Biol. 14: 1002515. PubMed
  47. Yang J, et al. 2022. Microbiome. 10:149. PubMed
  48. Bauer T, et al. 2021. Life (Basel). 11:. PubMed
  49. Bambouskova M, et al. 2021. Cell Reports. 34(10):108756. PubMed
  50. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  51. Platteel A, et al. 2017. Vaccine.. 10.1016/j.vaccine.2017.10.044. PubMed
  52. Liu J, et al. 2020. Nat Commun. 11:6396. PubMed
  53. Fan Q, et al. 2021. Bioact Mater. 6:4014. PubMed
  54. Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
  55. Biton M et al. 2018. Cell. 175(5):1307-1320 . PubMed
  56. Morales-Kastresana A, et al. 2020. Curr Protoc Cytom. 95:e81. PubMed
  57. Perry JL, et al. 2020. ACS Nano. 5.583333333. PubMed
  58. Pascual-Pasto G, et al. 2022. J Immunother Cancer. 10:. PubMed
  59. Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed
  60. Wang S, et al. 2021. Commun Biol. 22:. PubMed
  61. Dong R, et al. 2021. Frontiers in Immunology. 12:620238. PubMed
  62. Pazmandi K, et al. 2014. Free Radic Biol Med. 77:281. PubMed
  63. Kamata T, et al. 2020. Cell Reports. 31(12):107802. PubMed
  64. Sanchez M et al. 2017. Cell host & microbe. 22(4):543-551 . PubMed
  65. Huang L, et al. 2021. Frontiers in Cellular and Infection Microbiology. 10:606340. PubMed
  66. Guendel F, et al. 2020. Immunity. 53(5):1015-1032.e8. PubMed
  67. Zhao H, et al. 2022. Int J Biol Sci. 18:154. PubMed
  68. Roux C, et al. 2021. Antioxidants (Basel). 10:. PubMed
  69. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  70. Porrello A, et al. 2018. Nat Commun. 9:1988. PubMed
  71. Renner K, et al. 2020. Cell Reports. 29(1):135-150.e9.. PubMed
  72. Süß P, et al. 2020. Cell Reports. 30(12):4082-4095. PubMed
  73. Piirainen S, et al. 2021. Glia. 69:2459. PubMed
  74. Chen YL, et al. 2022. Front Neurosci. 16:876582. PubMed
  75. Cao H, et al. 2020. Front Pharmacol. 11:578943. PubMed
  76. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  77. Ioanna E Galani et al. 2017. Immunity. 46(5):875-890 . PubMed
  78. Chithanathan K, et al. 2022. Biology (Basel). 11:. PubMed
  79. Fontana C, et al. 2016. J Biol Chem. 291: 7727-7741. PubMed
RRID
AB_313322 (BioLegend Cat. No. 107607)
AB_313322 (BioLegend Cat. No. 107608)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
I-A/I-E
Specificity Alt (DOES NOT SHOW ON TDS):
I-A/I-E
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about I-A/I-E on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All I-A/I-E Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse I-A/I-E M5/114.15.2 FC
FITC anti-mouse I-A/I-E M5/114.15.2 FC
PE anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
PE/Cyanine5 anti-mouse I-A/I-E M5/114.15.2 FC
APC anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 488 anti-mouse I-A/I-E M5/114.15.2 FC,3D IHC
Alexa Fluor® 647 anti-mouse I-A/I-E M5/114.15.2 FC
Pacific Blue™ anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 700 anti-mouse I-A/I-E M5/114.15.2 FC,SB
PerCP/Cyanine5.5 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP anti-mouse I-A/I-E M5/114.15.2 FC
APC/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 421™ anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,SB
Brilliant Violet 510™ anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E (Maxpar® Ready) M5/114.15.2 FC,CyTOF®
Brilliant Violet 605™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 650™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 711™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 785™ anti-mouse I-A/I-E M5/114.15.2 FC
PE/Dazzle™ 594 anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 594 anti-mouse I-A/I-E M5/114.15.2 IHC-F,3D IHC
APC/Fire™ 750 anti-mouse I-A/I-E M5/114.15.2 FC
TotalSeq™-A0117 anti-mouse I-A/I-E M5/114.15.2 PG
Ultra-LEAF™ Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
TotalSeq™-B0117 anti-mouse I-A/I-E M5/114.15.2 PG
TotalSeq™-C0117 anti-mouse I-A/I-E M5/114.15.2 PG
Spark Blue™ 550 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 640 anti-mouse I-A/I-E M5/114.15.2 FC
Spark YG™ 581 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark UV™ 387 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Violet™ 538 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP/Fire™ 806 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Red™ 718 anti-mouse I-A/I-E M5/114.15.2 FC
APC/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark PLUS UV395™ anti-mouse I-A/I-E M5/114.15.2 FC
Go To Top Version: 4    Revision Date: 10/30/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account