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Alexa Fluor® 594 anti-Avi-tag Antibody

Pricing & Availability
Clone
W19258A (See other available formats)
Regulatory Status
RUO
Other Names
Avi-Tag, AviTag, Avi epitope Tag
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W19258A_A594_AviTag_Antibody_051823
CHO cells untransfected (left panel) or transfected (right panel) with an expression vector for VEGF-165-Avi-tag were fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton X-100, and blocked with 5% FBS for 1 hour. Cells were then intracellularly stained with Alexa Fluor® 594 anti-Avi-tag (clone W19258A) overnight at 4°C. Nuclei and actin filaments were counterstained with DAPI (Cat. No. 422801) and Flash Phalloidin™ Green 488 (Cat. No. 424201). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
  • W19258A_A594_AviTag_Antibody_051823
    CHO cells untransfected (left panel) or transfected (right panel) with an expression vector for VEGF-165-Avi-tag were fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton X-100, and blocked with 5% FBS for 1 hour. Cells were then intracellularly stained with Alexa Fluor® 594 anti-Avi-tag (clone W19258A) overnight at 4°C. Nuclei and actin filaments were counterstained with DAPI (Cat. No. 422801) and Flash Phalloidin™ Green 488 (Cat. No. 424201). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
Compare all formats See Alexa Fluor® 594 spectral data
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606758 100 µg $370
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Description

Avi-tag is a 15 amino acid sequence, and the minimal acceptor peptide of a larger protein domain that serves as a substrate for the biotin ligase BirA from E. coli. Although one of the major advantages of Avi-tag fusion proteins is a controlled biotinylation in this domain, Avi-tag is also a useful tool in cell and molecular biology.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Epitope tag
Reported Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

This clone recognizes proteins tagged with Avi-tag sequence irrespective of its biotinylation status.

Additional Product Notes

ICC: Cells were fixed with 4% PFA and permeabilized with Triton X-100 

RRID
AB_3068041 (BioLegend Cat. No. 606758)

Antigen Details

Structure
GLNDIFEAQKIEWHE. Avi-tag amino acid sequence
Distribution

Depends on the subcellular location of the tagged protein

Biology Area
Cell Biology, Protein Purification, Protein Trafficking and Clearance
Gene ID
NA
UniProt
View information about Avi-tag on UniProt.org
Go To Top Version: 2    Revision Date: 09/22/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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