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Purified anti-Avi-tag Antibody

Pricing & Availability
Clone
W19258A (See other available formats)
Regulatory Status
RUO
Other Names
Avi-Tag, AviTag, Avi epitope Tag
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A_W19258A_PURE_AviTag_Antibody_081222
Whole cell extracts (15 µg total protein/lane) from HEL cells spiked with 20 ng/lane of Avi-tagged VEGF-165 biotinylated or non-biotinylated recombinant protein, were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.5 µg/mL of purified anti-Avi-tag (clone W19258A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426318) was used as a detection agent. Lane M: Molecular weight marker
  • A_W19258A_PURE_AviTag_Antibody_081222
    Whole cell extracts (15 µg total protein/lane) from HEL cells spiked with 20 ng/lane of Avi-tagged VEGF-165 biotinylated or non-biotinylated recombinant protein, were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.5 µg/mL of purified anti-Avi-tag (clone W19258A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426318) was used as a detection agent. Lane M: Molecular weight marker
  • B_W19258A_PURE_AviTag_Antibody_2_081222
    Whole cell extracts (300 µg) total protein prepared from untransfected or VEGF-165-Avi-tag transfected CHO cell extracts were incubated overnight with 2 µg of purified rat IgG2b, κ isotype control or purified anti-Avi-tag (clone W19258A). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to PDGF membrane and probed with a different Avi-tag.
  • C_W19258A_PURE_AviTag_Antibody_3_041723
    CHO cells untransfected or transfected with an expression vector for VEGF-165-Avi-Tag were fixed with 4% paraformaldehyde, permeabilized with 0.5% TritonX-100, and blocked with 5% FBS for 1 hour. Cells were then intracellularly stained with purified anti-Avi-Tag (clone W19258A) overnight at 4°C, followed by incubation with Alexa Fluor® 647 anti-Rat IgG2b (Cat. No. 408208) at 2.0 µg/mL. Nuclei and actin filaments were counterstained with DAPI (Cat. No. 422801) and Flash Phalloidin™ Green 488 (cat. No. 424201). The images were captured in a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm.
  • D_W19258A_PURE_Avitag_Antibody_083122
    CHO cells transfected with an expression vector for VEGF-165-Avi-tag (filled histogram), and untransfected cells (solid line, open histogram) were fixed and permeabilized using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with purified anti-Avi-tag (solid line histograms) or purified rat IgG2b, κ isotype control (dashed line, open histogram) followed by PE goat anti-rat IgG.
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606751 25 µg $110
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606752 100 µg $275
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Description

Avi-tag is a 15 amino acid sequence, and the minimal acceptor peptide of a larger protein domain that serves as a substrate for the biotin ligase BirA from E. coli. Although one of the major advantages of Avi-tag fusion proteins is a controlled biotinylation in this domain, Avi-tag is also a useful tool in cell and molecular biology.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Epitope tag
Reported Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone recognizes proteins tagged with Avi-tag sequence irrespective of its biotinylation status.

Additional Product Notes

Antibody concentration per application is subject to the Avi-tag-recombinant protein. Titration is required for every specific protein.

RRID
AB_2924619 (BioLegend Cat. No. 606751)
AB_2924619 (BioLegend Cat. No. 606752)

Antigen Details

Structure
GLNDIFEAQKIEWHE. Avi-tag amino acid sequence
Distribution

Depends on the subcellular location of the tagged protein

Biology Area
Cell Biology, Protein Purification, Protein Trafficking and Clearance
Gene ID
NA
UniProt
View information about Avi-tag on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 09/22/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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