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Alexa Fluor® 647 anti-Avi-tag Antibody

Pricing & Availability
Clone
W19258A (See other available formats)
Regulatory Status
RUO
Other Names
Avi-Tag, AviTag, Avi epitope Tag
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A_W19258A_A647_AviTag_Antibody_ICFC_060623.png
CHO cells untransfected (negative control, left panel) or expressing VEGF-165-Avi-tag (positive control, right panel) were fixed and permeabilized using Cyto-Fast™ Fix/Perm buffer (Cat. No. 426803) and intracellularly stained with Alexa Fluor® 647 anti-Avi-tag (clone W19258A).
  • A_W19258A_A647_AviTag_Antibody_ICFC_060623.png
    CHO cells untransfected (negative control, left panel) or expressing VEGF-165-Avi-tag (positive control, right panel) were fixed and permeabilized using Cyto-Fast™ Fix/Perm buffer (Cat. No. 426803) and intracellularly stained with Alexa Fluor® 647 anti-Avi-tag (clone W19258A).
  • B_W19258A_A647_AviTag_Antibody_ICC_052523
    CHO cells untransfected (left panel) or transfected (right panel) with an expression vector for VEGF-165-Avi-tag were fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton X-100, and blocked with 5% FBS for 1 hour. Cells were then intracellularly stained with Alexa Fluor® 647 anti-Avi-tag (clone W19258A) overnight at 4°C. Nuclei and actin filaments were counterstained with DAPI (Cat. No. 422801) and Flash Phalloidin™ Green 488 (Cat. No. 424201). The images were captured in a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
606759 25 tests $183
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606762 100 µg $370
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606760 100 tests $382
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Description

Avi-tag is a 15 amino acid sequence, and the minimal acceptor peptide of a larger protein domain that serves as a substrate for the biotin ligase BirA from E. coli. Although one of the major advantages of Avi-tag fusion proteins is a controlled biotinylation in this domain, Avi-tag is also a useful tool in cell and molecular biology.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Epitope tag
Reported Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Rat
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
µg sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC (test sizes) - Quality tested
ICC (µg size) - Quality tested
Recommended Usage

Each lot of the test-sized antibody is quality control tested by intracellular flow cytometry using our Cyto-Fast™ Fix/Perm Buffer Set. For flow cytometric staining, the suggested use of this reagent is 5 µL per 106 cells in 100 µL volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

Each lot of the µg-sized antibody is quality control tested by immunocytochemistry. For immunocytochemistry using the ug size, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

This clone recognizes proteins tagged with Avi-tag sequence irrespective of its biotinylation status.

Additional Product Notes

ICFC: Cells were fixed and permeabilizes using Cyto -Fast™ Buffer system
ICC: Cells were fixed with 4% PFA and permeabilized with Triton X-100

RRID
AB_3068042 (BioLegend Cat. No. 606759)
AB_3068042 (BioLegend Cat. No. 606762)
AB_3068042 (BioLegend Cat. No. 606760)

Antigen Details

Structure
GLNDIFEAQKIEWHE. Avi-tag amino acid sequence
Distribution

Depends on the subcellular location of the tagged protein

Biology Area
Cell Biology, Protein Purification, Protein Trafficking and Clearance
Gene ID
NA
UniProt
View information about Avi-tag on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 09/22/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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