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Alexa Fluor® 647 anti-Tubulin β 3 (TUBB3) Antibody

Pricing & Availability
Clone
TUJ1 (See other available formats)
Regulatory Status
RUO
Other Names
CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, Tubulin beta-3 chain, tubulin beta-III, tubulin beta-4 chain, class III beta-tubulin
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1_TUJ1_A647_Tubulinbeta3_Antibody_040617
ICC staining of Alexa Fluor® 647 anti-Tubulin β 3 (TUBB3) antibody (clone TUJ1) on SH-SY5Y neuroblastoma cells. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% Normal Goat Serum. The cells were then stained with 1 µg/mL of the primary antibody for three hours at room temperature. Nuclei were counterstained with Hoechst 33342. The image was captured with a 60X objective.
  • 1_TUJ1_A647_Tubulinbeta3_Antibody_040617
    ICC staining of Alexa Fluor® 647 anti-Tubulin β 3 (TUBB3) antibody (clone TUJ1) on SH-SY5Y neuroblastoma cells. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% Normal Goat Serum. The cells were then stained with 1 µg/mL of the primary antibody for three hours at room temperature. Nuclei were counterstained with Hoechst 33342. The image was captured with a 60X objective.
  • 2_TUJ1_A647_Tubulinbeta3_Antibody_010818
    ICC staining of Alexa Fluor® 647 anti-Tubulin β 3 (TUBB3) (clone TUJ1) on SH-SY5Y neuroblastoma cells. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then stained with 1 µg/ml of the primary antibody for three hours at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective.
  • 3_TUJ1_A647_Tubulinbeta3_Antibody_3D-IHC_092121
    Paraformaldehyde-fixed (4%), 500 μm-thick mouse kidney section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-mouse Podoplanin Antibody (clone PMab-1) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse CD31 Antibody (clone MEC13.3) Alexa Fluor® 594 at 5 µg/mL (orange), and anti-Tubulin β 3 (TUBB3) Antibody (Clone TUJ1) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 20X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
    Watch the video.
  • 4-TUJ1_A647_Tubulinbeta3_Antibody_IHC-P_030823
    IHC staining of Alexa Fluor® 647 anti-Tubulin β 3 (TUBB3) (clone TUJ1) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Tris-EDTA pH 9.0, the tissue was incubated without (panel A) and with (panel B) 5.0 µg/mL of Alexa Fluor® 647 anti-Tubulin β 3 (TUBB3) (clone TUJ1) (magenta). Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). Images were captured with a 10X objective. Scale bar: 50 µm
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801209 25 µg $141
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801210 100 µg $352
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Description

Tubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against microtubules derived from rat brain.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
ICFC, 3D IHC, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 5.0 µg per million cells. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: flow cytometry4, immunofluorescence microscopy1-5,7, immunohistochemistry5,7, and Western blotting8.

This antibody is well characterized and highly reactive to neuron specific Class III ß-tubulin (ßIII). TUJ1 does not identify ß-tubulin found in glial cells. TUJ1 recognizes an epitope located within the last 15 C-terminal residues8.

Application References

(PubMed link indicates BioLegend citation)
  1. Nishimura K, et al. 2017. PLoS One. 12(1): e0170568. (ICC)
  2. Jongbloets J, et al. 2017. Nat Commun. 8: 14666. (ICC) PubMed
  3. Liu W.J, et al. 2015. Eur J Histochem. 59(1): 2464. (ICC) PubMed
  4. Chintalapudi SR, et al. 2016. Front Aging Neursci. 8:93. (FC, ICC) PubMed
  5. Ambasudhan R, et al. 2011. Cell Stem Cell. 9(2):113. (IHC, ICC)
  6. Hu X., et al. 2006. Nature Neuroscicene. 9(12):1520. (WB) PubMed
  7. Zechner D., et al. 2003. Develop Biology. 258(2):406. (ICC, IHC)
  8. Lee MK, et al. 1990. Proc. Natl. Acad. Sci. USA 18:7195. (WB)
Product Citations
  1. Li W, et al. 2022. Nat Commun. 13:7812. PubMed
  2. Citro A, et al. 2023. Nat Commun. 14:878. PubMed
  3. Arsić A, et al. 2020. Sci Rep. 10:6441. PubMed
  4. Holman HA, et al. 2019. Front Cell Neurosci. 13:186. PubMed
  5. Funato K, et al. 2021. Cell Stem Cell. 28(5):894-905.e7. PubMed
  6. Bloom ML, et al. 2020. Chemical Senses. 45(5):333-346. PubMed
RRID
AB_2686930 (BioLegend Cat. No. 801209)
AB_2686930 (BioLegend Cat. No. 801210)

Antigen Details

Structure
Tubulin β 3 is a 450 amino acid protein with a molecular mass of ~50 kD.
Distribution

Tissue distribution: central and peripheral nervous system.
Cellular distribution: cytosol, cytoskeleton and nucleus.

Function
Tubulin β 3 is the major constituent of microtubules, and plays a critical role in proper axon guidance and maintenance.
Interaction
Alpha tubulin, kinesin and dynein.
Cell Type
Mature Neurons, Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Microtubules
Antigen References

1. Zhao X, et al. 2017. Med Sci Monit. 22: 3915.
2. Lebok P, et al. 2016. Oncol Lett. 11(3):1987.
3. Du J, et al. 2015. BMC Cancer. 15:536. PubMed
4. Rogue DM., et al. 2013. Clin Exp Metastasis. 31(1): 101.
5. Ploussard G, et al. 2010. Cancer Res. 70(22):9253. PubMed

Gene ID
10381 View all products for this Gene ID
UniProt
View information about Tubulin beta-3 on UniProt.org
Go To Top Version: 3    Revision Date: 03/08/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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