- Clone
- 8.1.1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T1a, gp38, stromal cell marker
- Isotype
- Syrian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![8-1-1_BV421_Podoplanin_Antibody_081519 8-1-1_BV421_Podoplanin_Antibody_081519](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/8-1-1_BV421_Podoplanin_Antibody_081519.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Mouse thymic epithelial stromal cell line TE-71 stained with Podoplanin (clone 8.1.1) Brilliant Violet 421™ (filled histogram) or anti-hamster (Syrian) IgG Brilliant Violet 421™ isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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127423 | 50 µg | $253 |
The mucin-type glycoprotein podoplanin is thought to be involved in the development of the lymphatic vascular system. Podoplanin is named after its expression in the kidney glomerular epithelial cells (podocytes). It has a potential role in tumor progression.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Syrian Hamster
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemistry6, and spatial biology (IBEX)8,9.
-
Application References
(PubMed link indicates BioLegend citation) -
- Farr A, et al. 1992. J. Histochem. Cytochem. 40:651.
- Farr AG, et al. 1992. J. Exp. Med. 176:1477.
- Bekiaris V, et al. 2008. J. Immunol. 180:6768.
- Algars A, et al. 2011. Blood 117:4387. PubMed
- Reis VO, et al. 2012. Immunobiology. 217:831. PubMed
- Kaji C, et al. 2012. Acta. Histochem. Cytochem. 45:227. (IHC)
- Kretschmer S, et al. 2013. PLoS One. 8:e52201. PubMed.
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
-
- RRID
-
AB_2814017 (BioLegend Cat. No. 127423)
Antigen Details
- Structure
- 43 Kd glycosylated type-1 transmembrane protein. Mucin-type protein.
- Distribution
-
Expressed on epithelial and mesothelial cells.
- Cell Type
- Epithelial cells
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
- Antigen References
-
1. Farr A, et al. 1992. J. Histochem. Cytochem. 40:651.
2. Schacht V, et al. 2005. Am. J. Pathol. 166:913. - Gene ID
- 14726 View all products for this Gene ID
- UniProt
- View information about Podoplanin on UniProt.org
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
-
It is lot-specific. On average it ranges between 2-4.
Other Formats
View All Podoplanin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Biotin anti-mouse Podoplanin | 8.1.1 | FC |
Purified anti-mouse Podoplanin | 8.1.1 | FC,IHC-F,SB |
Alexa Fluor® 488 anti-mouse Podoplanin | 8.1.1 | FC |
PE anti-mouse Podoplanin | 8.1.1 | FC |
APC anti-mouse Podoplanin | 8.1.1 | FC |
PE/Cyanine7 anti-mouse Podoplanin | 8.1.1 | FC |
Alexa Fluor® 594 anti-mouse Podoplanin | 8.1.1 | ICC,IHC-F |
FITC anti-mouse Podoplanin | 8.1.1 | FC |
APC/Cyanine7 anti-mouse Podoplanin | 8.1.1 | FC |
PE/Dazzle™ 594 anti-mouse Podoplanin | 8.1.1 | FC |
PerCP/Cyanine5.5 anti-mouse Podoplanin | 8.1.1 | FC |
Brilliant Violet 421™ anti-mouse Podoplanin | 8.1.1 | FC |
TotalSeq™-A1035 anti-mouse Podoplanin | 8.1.1 | PG |
APC/Fire™ 750 anti-mouse Podoplanin | 8.1.1 | FC |
TotalSeq™-C1035 anti-mouse Podoplanin | 8.1.1 | PG |
TotalSeq™-B1035 anti-mouse Podoplanin | 8.1.1 | PG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-mouse Podoplanin
Mouse thymic epithelial stromal cell line TE-71 stained with... -
Purified anti-mouse Podoplanin
TE-71 cells stained with purified 8.1.1, followed by anti-Sy... Fresh, frozen mouse spleen was stained with purified Podopla... Mice were injected subcutaneously with sheep red blood cells... -
Alexa Fluor® 488 anti-mouse Podoplanin
TE-71 mouse thymic epithelial stromal cell line stained with... -
PE anti-mouse Podoplanin
TE-71, mouse thymic epithelial stromal cell line, stained wi... -
APC anti-mouse Podoplanin
Mouse thymic epithelial stromal cell line TE-71 stained with... -
PE/Cyanine7 anti-mouse Podoplanin
Mouse thymic epithelial stromal cell line TE-71 stained with... -
Alexa Fluor® 594 anti-mouse Podoplanin
TE-71 mouse thymic epithelial stromal cell line cells were f... C57BL/6 mouse frozen kidney section was fixed with 4% parafo... -
FITC anti-mouse Podoplanin
Mouse thymic epithelial stromal cell line TE-71 stained with... -
APC/Cyanine7 anti-mouse Podoplanin
Mouse thymic epithelial stromal cell line TE-71 stained with... -
PE/Dazzle™ 594 anti-mouse Podoplanin
Mouse thymic epithelial stromal cell line TE-71 stained with... -
PerCP/Cyanine5.5 anti-mouse Podoplanin
Mouse thymic epithelial stromal cell line TE-71 stained with... -
Brilliant Violet 421™ anti-mouse Podoplanin
Mouse thymic epithelial stromal cell line TE-71 stained with... -
TotalSeq™-A1035 anti-mouse Podoplanin
-
APC/Fire™ 750 anti-mouse Podoplanin
Mouse thymic epithelial stromal cell line TE-71 was stained ... -
TotalSeq™-C1035 anti-mouse Podoplanin
-
TotalSeq™-B1035 anti-mouse Podoplanin
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