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PE anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
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Product Citations
publications
17A2_PE_021606
C57BL/6 mouse splenocytes stained with 17A2 PE
  • 17A2_PE_021606
    C57BL/6 mouse splenocytes stained with 17A2 PE
Compare all formats See PE spectral data
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100205 50 µg $61
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100206 200 µg $143
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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Xue G, et al. 2021. Cancer Cell. 39:1610. PubMed
  2. Hiyoshi H, et al. 2022. Cell Host Microbe. 30:163. PubMed
  3. Chan WY, et al. 2022. Infect Immun. 90:e0084618a. PubMed
  4. Wang D, et al. 2022. Cell Rep. 39:110587. PubMed
  5. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  6. Yang K, et al. 2022. Sci Immunol. 7:eabn2888. PubMed
  7. McAlpine CS, et al. 2022. J Exp Med. 219: . PubMed
  8. TaSli PN, et al. 2022. J Food Biochem. 46:e14393. PubMed
  9. Shen Z, et al. 2022. EBioMedicine. 85:104285. PubMed
  10. Deng P, et al. 2022. J Clin Invest. 132: . PubMed
  11. Wei M, et al. 2022. Front Immunol. 13:1017574. PubMed
  12. Domingos-Pereira S, et al. 2022. Int J Mol Sci. 24: . PubMed
  13. Giannou AD, et al. 2023. Immunity. 56:125. PubMed
  14. Briukhovetska D, et al. 2023. Immunity. 56:143. PubMed
  15. Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed
  16. Zuchtriegel G, et al. 2016. PLoS Biol. 14: 1002459. PubMed
  17. Xu C, et al. 2017. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2017.05.071. PubMed
  18. Zhao L, et al. 2022. Oncogene. 41:4200. PubMed
  19. Guo D, et al. 2023. Cell Biosci. 13:51. PubMed
  20. Fan NW, et al. 2023. FASEB J. 37:e22855. PubMed
  21. Zhang X, et al. 2023. Front Immunol. 14:1116749. PubMed
  22. Sun JY, et al. 2023. Sci Adv. 9:eade4110. PubMed
  23. Shi Y, et al. 2023. Nat Commun. 14:1884. PubMed
  24. Fiedler T, et al. 2023. J Neuroinflammation. 20:100. PubMed
  25. Li Y, et al. 2023. Front Cell Infect Microbiol. 13:1117230. PubMed
  26. Bhaskar A, et al. 2023. iScience. 26:106644. PubMed
  27. Wang Y, et al. 2023. iScience. 26:106630. PubMed
  28. Milighetti M, et al. 2023. iScience. 26:106937. PubMed
  29. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  30. Li Q, et al. 2022. Cell Rep. 40:111308. PubMed
  31. Jong RM, et al. 2022. J Immunol. 208:407. PubMed
  32. Correa S, et al. 2020. ACS Nano. 14:2224. PubMed
  33. Gonalves S, et al. 2021. Cell Reports. 34(11):108860. PubMed
  34. Reboldi A, et al. 2016. Sci Transl Med. 352: aaf4822. PubMed
  35. Gazit R, et al. 2014. J Exp Med. 211:1315. PubMed
  36. Baryawno N et al. 2019. Cell. 177(7):1915-1932 . PubMed
  37. Shen S, et al. 2018. Pain Med. 19:686. PubMed
  38. Ushijima M, et al. 2018. Front Immunol. 9:243. PubMed
  39. Xu ZH, et al. 2021. Mediators Inflamm. 2021:8856326. PubMed
  40. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  41. Kunimura K, et al. 2020. Cell Reports. 29(9):2823-2834.e7.. PubMed
  42. Bedi B, et al. 2012. Proc Natl Acad Sci U S A. 109:275. PubMed
  43. Shu T, et al. 2022. Eur Respir J. Online ahead of print. PubMed
  44. Al-Rifai R, et al. 2022. Nat Commun. 13:6592. PubMed
  45. Jazowiecka-Rakus J, et al. 2021. Cancers (Basel). 13:. PubMed
  46. Liang W, et al. 2020. Nat Commun. 4.563888889. PubMed
  47. Lutzmann M, et al. 2019. Cell Rep. 28:2851. PubMed
  48. Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed
  49. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  50. Kuang Z, et al. 2020. Antib Ther. 3:227. PubMed
  51. Hu D, et al. 2021. J Cell Mol Med. 25:2900. PubMed
  52. Mathur S, et al. 2021. JCI Insight. 6:. PubMed
  53. Ohyagi M, et al. 2021. Nat Commun. 12:7344. PubMed
  54. Yan X, et al. 2022. Int J Biol Sci. 18:585. PubMed
  55. Helm M, et al. 2022. Life (Basel). 12:. PubMed
  56. Tu X, et al. 2022. Nat Commun. 13:6977. PubMed
  57. Schmidt D, et al. 2016. PLoS One. 11: 0159059. PubMed
  58. He C, et al. 2020. Clin Transl Med. 10:e39. PubMed
  59. Ma C, et al. 2018. Science. 360:eaan5931. PubMed
  60. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  61. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  62. Biram A, et al. 2020. Cell Rep. 30:1910. PubMed
  63. Delgado‐Benito V et al. 2018. Molecular cell. 72(4):636-649 . PubMed
  64. Mariani SA, et al. 2019. Immunity. 50:1439. PubMed
  65. Wang F, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:257. PubMed
  66. Zhou Y, et al. 2022. Nat Commun. 13:2906. PubMed
  67. Stutchfield B, et al. 2015. Gastroenterology. 149: 1896-1909.e14. PubMed
  68. Koren N, et al. 2021. Cell Host Microbe. 29(2):197-209.e5. PubMed
  69. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  70. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  71. McAlpine CS, et al. 2021. Nature. 595:701. PubMed
  72. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  73. Goldman E, et al. 2021. Cells. 10:. PubMed
  74. Cassidy BR, et al. 2020. J Neuroinflammation. 17:259. PubMed
  75. Wu J, et al. 2020. Immunity. 53:115. PubMed
  76. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  77. Lin H, et al. 2019. Infect Immun. 87:e00055-19. PubMed
  78. Pedraza–Zamora CP, et al. 2017. Parasite Immunol. 39:4. PubMed
  79. Marinescu CI, et al. 2021. Stem Cell Res Ther. 12:319. PubMed
  80. Shi R, et al. 2022. Theranostics. 12:875. PubMed
  81. Cheng B, et al. 2022. Cancer Commun (Lond). 42:17. PubMed
  82. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  83. Hu J, et al. 2022. J Neuroinflammation. 19:71. PubMed
  84. Chen Y, et al. 2015. PLoS One. 10: 0135217. PubMed
  85. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  86. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  87. Doonan J, et al. 2019. Nat Commun. 10:1554. PubMed
  88. Halpern KB, et al. 2018. Nat Biotechnol. 36:962. PubMed
  89. Deng M, et al. 2020. Nat Commun. 11:2193. PubMed
  90. Paul S, et al. 2018. Appl Microbiol Biotechnol. 102:8895. PubMed
  91. Fang E, et al. 2022. MedComm (2020). 3:e117. PubMed
  92. Harnett MM, et al. 2022. Front Immunol. 13:953053. PubMed
  93. Zuo S, et al. 2021. J Immunother Cancer. 9:. PubMed
  94. O'Connor T, et al. 2020. Cancer Cell. 36(3):250-267. PubMed
  95. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  96. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  97. Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed
  98. Kim JH, et al. 2019. Mol Med. 25:33. PubMed
  99. Pedraza-Zamora C, et al. 2017. Parasite Immunol. 10.1111/pim.12420. PubMed
  100. Huang Z, et al. 2021. Nat Commun. 12:145. PubMed
  101. Cho K, et al. 2021. iScience. 24:103117. PubMed
  102. Zhou Z, et al. 2022. Infect Immun. 90:e0045321. PubMed
  103. Bullard BL, et al. 2022. NPJ Vaccines. 7:65. PubMed
  104. Tomala J, et al. 2020. Methods Mol Biol. 2111:101. PubMed
  105. Nikolos F, et al. 2022. Nat Commun. 13:1487. PubMed
  106. Lewandowski JP, et al. 2020. Genome Biol. 1.039583333. PubMed
  107. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  108. Xiang W, et al. 2018. Nat Commun. 9:2574. PubMed
  109. Sanmarco LM, et al. 2021. Nature. 590:473. PubMed
  110. Rozanski C, et al. 2015. J Immunol. 194:4717. PubMed
  111. Zou D, et al. 2020. EBioMedicine. 52:102652. PubMed
  112. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  113. Feng Y, et al. 2022. J Nanobiotechnology. 20:193. PubMed
  114. Fang H, et al. 2020. Nat Commun. 4.661805556. PubMed
  115. Tummers B, et al. 2020. Immunity. 52(6):994-1006.e8. PubMed
  116. Sun J et al. 2018. Cell stem cell. 23(3):355-369 . PubMed
  117. Huang L, et al. 2018. Exp Neurol. 300:41. PubMed
  118. Ding Y, et al. 2020. Evid Based Complement Alternat Med. 2020:9521073. PubMed
  119. Zhang T, et al. 2020. Cell. 180(6):1115-1129. PubMed
  120. Wang W, et al. 2021. J Am Heart Assoc. 10:e019142. PubMed
  121. Olson B, et al. 2021. Brain Behav Immun. 97:102. PubMed
  122. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  123. Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed
  124. Yamamura K, et al. 2017. Nat Commun. 8:13946. PubMed
  125. Hsieh Y, et al. 2014. Infect Immun . 82:2087. PubMed
  126. Winchell CG, et al. 2020. Front Immunol. 1.077083333. PubMed
  127. Chan WY, et al. 2019. Infect Immun. 87:. PubMed
  128. Zhang MS, et al. 2022. Nat Commun. 13:954. PubMed
  129. Jazowiecka-Rakus J, et al. 2020. Mol Ther Oncolytics. 18:335. PubMed
  130. Chen H, et al. 2022. Front Pharmacol. 13:827520. PubMed
  131. Schneider A, et al. 2014. J Leukoc Biol. 96:857. PubMed
  132. Yao C, et al. 2018. Autophagy. 1.854861111. PubMed
  133. Kim MY, et al. 2022. Nat Commun. 13:3296. PubMed
  134. Zhou H, et al. 2021. Cell Death Discov. 7:332. PubMed
  135. Zhou T, et al. 2021. Bioact Mater. 0.734027778. PubMed
  136. Rodríguez L, et al. 2021. Biomolecules. 11: . PubMed
  137. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  138. Lu C, et al. 2020. Cancer Cell. 39(1):96-108.e6. PubMed
  139. Uluçkan ö, et al. 2020. Cell Reports. 29(4):844-859.e3.. PubMed
  140. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  141. Maulhardt HA, et al. 2020. Invest New Drugs. 1618:38. PubMed
  142. Yu H, et al. 2015. PLoS One. 10: 0143001. PubMed
  143. Fathman J, et al. 2010. Proc Natl Acad Sci U S A. 107:3663. PubMed
  144. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  145. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  146. Gawish R, et al. 2022. Elife. 11:. PubMed
  147. Vannini N, et al. 2016. Nat Commun. 7:13125. PubMed
  148. Wu L, et al. 2020. Cancer Immunol Res. 710:8. PubMed
  149. Das A, et al. 2020. J Bone Miner Res. 36:199. PubMed
  150. Crowe J, et al. 2020. PLoS Pathog. 16:e1008391. PubMed
  151. He C, et al. 2021. Immunol Cell Biol. 99:611. PubMed
  152. Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
  153. Zuo S, et al. 2021. EBioMedicine. 64:103240. PubMed
  154. Zeng F, et al. 2021. J Invest Dermatol. 596:141. PubMed
  155. Guo S, et al. 2021. Frontiers in Immunology. 11:614667. PubMed
  156. Xiang W, et al. 2020. Signal Transduct Target Ther. 0.374305556. PubMed
  157. Hayashi K, et al. 2020. Nat Commun. 4.832638889. PubMed
RRID
AB_312662 (BioLegend Cat. No. 100205)
AB_312662 (BioLegend Cat. No. 100206)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-mouse CD3 17A2 FC
PE anti-mouse CD3 17A2 FC
Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Alexa Fluor® 647 anti-mouse CD3 17A2 FC,IHC-F,3D IHC,SB
Alexa Fluor® 488 anti-mouse CD3 17A2 FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse CD3 17A2 FC
Alexa Fluor® 700 anti-mouse CD3 17A2 FC
PerCP/Cyanine5.5 anti-mouse CD3 17A2 FC
PE/Cyanine7 anti-mouse CD3 17A2 FC
APC/Cyanine7 anti-mouse CD3 17A2 FC
Brilliant Violet 421™ anti-mouse CD3 17A2 FC,ICC
Brilliant Violet 570™ anti-mouse CD3 17A2 FC
Brilliant Violet 650™ anti-mouse CD3 17A2 FC
Brilliant Violet 785™ anti-mouse CD3 17A2 FC
Brilliant Violet 510™ anti-mouse CD3 17A2 FC
APC anti-mouse CD3 17A2 FC
Ultra-LEAF™ Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Brilliant Violet 605™ anti-mouse CD3 17A2 FC
Alexa Fluor® 594 anti-mouse CD3 17A2 IHC-F,FC,3D IHC,SB
Brilliant Violet 711™ anti-mouse CD3 17A2 FC
Biotin anti-mouse CD3 17A2 FC,IHC-F
PE/Dazzle™ 594 anti-mouse CD3 17A2 FC
APC/Fire™ 750 anti-mouse CD3 17A2 FC
Brilliant Violet 750™ anti-mouse CD3 17A2 FC
TotalSeq™-A0182 anti-mouse CD3 17A2 PG
TotalSeq™-B0182 anti-mouse CD3 17A2 PG
Spark Blue™ 550 anti-mouse CD3 17A2 FC
Spark NIR™ 685 anti-mouse CD3 17A2 FC
TotalSeq™-C0182 anti-mouse CD3 17A2 PG
APC/Fire™ 810 anti-mouse CD3 17A2 FC
PE/Fire™ 640 anti-mouse CD3 17A2 FC
Spark YG™ 570 anti-mouse CD3 17A2 IHC-F
PE/Fire™ 700 anti-mouse CD3 17A2 FC
PE/Cyanine5 anti-mouse CD3 17A2 FC
Spark Blue™ 574 anti-mouse CD3 Antibody 17A2 FC
Spark Violet™ 423 anti-mouse CD3 17A2 FC
PE/Fire™ 810 anti-mouse CD3 17A2 FC
Spark Red™ 718 anti-mouse CD3 17A2 FC
Spark UV™ 387 anti-mouse CD3 17A2 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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