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PE/Cyanine7 anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
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Product Citations
publications
17A2_PECy7_083105
C57BL/6 splenocytes stained with 17A2 PE/Cyanine7
  • 17A2_PECy7_083105
    C57BL/6 splenocytes stained with 17A2 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
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100219 25 µg $105
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100220 100 µg $248
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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Luo J, et al. 2022. J Nanobiotechnology. 20:228. PubMed
  2. Ramos SA, et al. 2022. J Allergy Clin Immunol. 149:767. PubMed
  3. Friesen M, et al. 2022. Brain Pathol. 32:e13032. PubMed
  4. Blanco LP, et al. 2022. Arthritis Rheumatol. 74:1971. PubMed
  5. Millar FR, et al. 2022. Cell Rep. 41:111596. PubMed
  6. Tran TA, et al. 2022. Front Immunol. 13:1007285. PubMed
  7. Lee CY, et al. 2022. J Invest Dermatol. :. PubMed
  8. Phares TW, et al. 2022. Front Pharmacol. 13:1029636. PubMed
  9. Montilla A, et al. 2023. Cell Death Dis. 14:16. PubMed
  10. Zhu X, et al. 2023. Front Immunol. 14:1114802. PubMed
  11. Ryan CB, et al. 2022. Neurobiol Dis. 163:105608. PubMed
  12. Georgiev GI, et al. 2022. Cell Chem Biol. 29:811. PubMed
  13. Xiao R, et al. 2022. J Immunol. 208:2109. PubMed
  14. Ramdas B, et al. 2022. Mol Ther. 30:2505. PubMed
  15. Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed
  16. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  17. Ho J, et al. 2016. PLoS Biol. 14:e2000117. PubMed
  18. Djokić V, et al. 2019. Front Immunol. 9:2891. PubMed
  19. Sanmarco LM, et al. 2022. Nature. 611:801. PubMed
  20. Barsheshet Y, et al. 2022. Int J Mol Sci. 23:. PubMed
  21. Swan SL, et al. 2023. Front Immunol. 14:1085547. PubMed
  22. Sakae H, et al. 2023. Cells. 12:. PubMed
  23. Dona MSI, et al. 2023. Basic Res Cardiol. 118:11. PubMed
  24. Zhang YT, et al. 2023. Front Immunol. 14:1085456. PubMed
  25. Bhaskar A, et al. 2023. iScience. 26:106644. PubMed
  26. Wang Y, et al. 2023. iScience. 26:106630. PubMed
  27. Nguyen T, et al. 2023. Nat Commun. 14:3357. PubMed
  28. Xiao C, et al. 2021. Front Pharmacol. 12:721273. PubMed
  29. Qi Z, et al. 2022. Nat Commun. 13:182. PubMed
  30. d'Errico P, et al. 2021. Nat Neurosci. Online ahead of print. PubMed
  31. Jacoby E, et al. 2021. NPJ Regen Med. 6:58. PubMed
  32. Ercoli G, et al. 2021. Front Immunol. 11:611661. PubMed
  33. Schneider C, et al. 2018. Cell. 174:271. PubMed
  34. Page N, et al. 2018. Immunity. 48:937. PubMed
  35. Vacca M, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01462. PubMed
  36. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
  37. Pan Y, et al. 2021. Front Cell Neurosci. 15:664312. PubMed
  38. Chen L, et al. 2022. Mol Ther Oncolytics. 24:522. PubMed
  39. Chen S, et al. 2022. Cancer Discov. :. PubMed
  40. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
  41. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  42. De Vlaeminck Y, et al. 2020. Cancers (Basel). 12:. PubMed
  43. Liang Y, et al. 2020. J Immunol. 204:2984. PubMed
  44. Tilstam PV, et al. 2021. J Clin Invest. 131:. PubMed
  45. Feizi N, et al. 2021. Cell Death Dis. 12:1026. PubMed
  46. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  47. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  48. Tanner MA, et al. 2021. Am J Physiol Heart Circ Physiol. 321:H633. PubMed
  49. Tian X, et al. 2022. Front Immunol. 13:875236. PubMed
  50. Patnode M, et al. 2013. J Biol Chem. 288:26533. PubMed
  51. Chan YH, et al. 2019. EMBO Mol Med. 11:e10092. PubMed
  52. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  53. Jia L, et al. 2022. Front Immunol. 13:897879. PubMed
  54. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  55. Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed
  56. Dieterich LC, et al. 2019. International Journal of Cancer. 145(10):2804-2815. PubMed
  57. Chew W, et al. 2016. Nat Methods. 13: 868-874. PubMed
  58. Campana L, et al. 2018. J Immunol. 200:1169. PubMed
  59. Green CD et al. 2018. Developmental cell. 46(5):651-667 . PubMed
  60. Wang F, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:257. PubMed
  61. Mooney J, et al. 2015. Sci Rep. 5: 14603. PubMed
  62. Luan HH et al. 2019. Cell. 178(5):1231-1244 . PubMed
  63. Chen D, et al. 2020. Cancer Immunol Res. 8:883. PubMed
  64. Taube C, et al. 2011. PLoS One. 6:e21799. PubMed
  65. Miska J et al. 2019. Cell reports. 27(1):226-237 . PubMed
  66. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  67. Garcia LR, et al. 2021. Nat Commun. 12:3364. PubMed
  68. Wessel AW, et al. 2020. Nat Commun. 4.123611111. PubMed
  69. Hu Y, et al. 2022. J Nanobiotechnology. 20:417. PubMed
  70. Ullah I, et al. 2021. Immunity. 54:2143. PubMed
  71. Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed
  72. Luo J, et al. 2022. J Nanobiotechnology. 20:228. PubMed
  73. Kulkarni R, et al. 2021. PLoS One. 16:e0257191. PubMed
  74. Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
  75. Mulas F, et al. 2020. Cell Mol Immunol. . PubMed
  76. Kishore M et al. 2017. Immunity. 47(5):875-889 . PubMed
  77. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  78. De Veirman K, et al. 2021. Oncoimmunology. 10:2000699. PubMed
  79. Jiang Y, et al. 2020. Adv Mater. 32:e2001808. PubMed
  80. Shannon JP, et al. 2021. STAR Protoc. 2:100790. PubMed
  81. Zeng W, et al. 2021. STAR Protocols. 2(1):100361. PubMed
  82. Li Z, et al. 2018. Front Immunol. 9:347. PubMed
  83. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  84. Yazlovitskaya EM, et al. 2021. J Cell Sci. 134:. PubMed
  85. Yang R, et al. 2022. J Adv Res. 35:259. PubMed
  86. Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed
  87. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
  88. M H, et al. 2016. Sci Rep. 6: 20588. PubMed
  89. Song P, et al. 2018. Int J Mol Med. 41:935. PubMed
  90. Hu Y, et al. 2021. Cell Death Dis. 12:743. PubMed
  91. Spix B, et al. 2022. Nat Commun. 13:318. PubMed
  92. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  93. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  94. Takaku S, et al. 2022. Evid Based Complement Alternat Med. 2022:6122955. PubMed
  95. Wieghofer P, et al. 2021. EMBO J. 40:e105123. PubMed
  96. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  97. Chitu V, et al. 2020. Cell Reports. 30(9):3004-3019. PubMed
  98. Burgener SS, et al. 2019. Cell Rep. 27:3646. PubMed
  99. Marcandalli J et al. 2019. Cell. 176(6):1420-1431 . PubMed
  100. Chen Z, et al. 2019. Mol Med Rep. 19:3210. PubMed
  101. Li E, et al. 2021. Front Immunol. 12:667177. PubMed
  102. Glasenapp A, et al. 2021. Circ Res. 129:369. PubMed
  103. Haase P, et al. 2020. Eur J Immunol. 50:558. PubMed
  104. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
  105. Kung YJ, et al. 2022. J Biomed Sci. 29:57. PubMed
  106. Akoolo L, et al. 2021. Cell Microbiol. 23:e13350. PubMed
  107. Namkoong H, et al. 2018. PLoS Pathog. 14:e1006955. PubMed
  108. Wawrzyniak M, et al. 2021. Pharmacol Res Perspect. 9:e00837. PubMed
  109. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  110. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  111. Blaszczak AM, et al. 2020. Immunometabolism. 2:00. PubMed
  112. Eastman AE, et al. 2020. Cell Rep. 31:107804. PubMed
  113. Reed-Geaghan EG, et al. 2020. J Exp Med. 217:00:00. PubMed
  114. Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed
  115. Zhao Y, et al. 2020. Front Immunol. 2.572222222. PubMed
  116. Li H, et al. 2022. iScience. 25:104481. PubMed
  117. Catrysse L, et al. 2021. Cell Rep. 36:109748. PubMed
  118. Carson W, et al. 2011. PLoS One. 6:e20385. PubMed
  119. Spevak CC, et al. 2020. Cell Stem Cell. 26(5):755-765. PubMed
  120. Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
  121. Tseng SH, et al. 2021. J Biomed Sci. 28:63. PubMed
  122. Hohsfield LA, et al. 2021. Elife. 10:. PubMed
RRID
AB_1732057 (BioLegend Cat. No. 100219)
AB_1732057 (BioLegend Cat. No. 100220)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-mouse CD3 17A2 FC
PE anti-mouse CD3 17A2 FC
Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Alexa Fluor® 647 anti-mouse CD3 17A2 FC,IHC-F,3D IHC,SB
Alexa Fluor® 488 anti-mouse CD3 17A2 FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse CD3 17A2 FC
Alexa Fluor® 700 anti-mouse CD3 17A2 FC
PerCP/Cyanine5.5 anti-mouse CD3 17A2 FC
PE/Cyanine7 anti-mouse CD3 17A2 FC
APC/Cyanine7 anti-mouse CD3 17A2 FC
Brilliant Violet 421™ anti-mouse CD3 17A2 FC,ICC
Brilliant Violet 570™ anti-mouse CD3 17A2 FC
Brilliant Violet 650™ anti-mouse CD3 17A2 FC
Brilliant Violet 785™ anti-mouse CD3 17A2 FC
Brilliant Violet 510™ anti-mouse CD3 17A2 FC
APC anti-mouse CD3 17A2 FC
Ultra-LEAF™ Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Brilliant Violet 605™ anti-mouse CD3 17A2 FC
Alexa Fluor® 594 anti-mouse CD3 17A2 IHC-F,FC,3D IHC,SB
Brilliant Violet 711™ anti-mouse CD3 17A2 FC
Biotin anti-mouse CD3 17A2 FC,IHC-F
PE/Dazzle™ 594 anti-mouse CD3 17A2 FC
APC/Fire™ 750 anti-mouse CD3 17A2 FC
Brilliant Violet 750™ anti-mouse CD3 17A2 FC
TotalSeq™-A0182 anti-mouse CD3 17A2 PG
TotalSeq™-B0182 anti-mouse CD3 17A2 PG
Spark Blue™ 550 anti-mouse CD3 17A2 FC
Spark NIR™ 685 anti-mouse CD3 17A2 FC
TotalSeq™-C0182 anti-mouse CD3 17A2 PG
APC/Fire™ 810 anti-mouse CD3 17A2 FC
PE/Fire™ 640 anti-mouse CD3 17A2 FC
Spark YG™ 570 anti-mouse CD3 17A2 IHC-F
PE/Fire™ 700 anti-mouse CD3 17A2 FC
PE/Cyanine5 anti-mouse CD3 17A2 FC
Spark Blue™ 574 anti-mouse CD3 Antibody 17A2 FC
Spark Violet™ 423 anti-mouse CD3 17A2 FC
PE/Fire™ 810 anti-mouse CD3 17A2 FC
Spark Red™ 718 anti-mouse CD3 17A2 FC
Spark UV™ 387 anti-mouse CD3 17A2 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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