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Purified anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
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Product Citations
publications
17A2_Pure_021606
C57BL/6 mouse splenocytes stained with purified 17A2, followed by anti-rat IgG FITC
  • 17A2_Pure_021606
    C57BL/6 mouse splenocytes stained with purified 17A2, followed by anti-rat IgG FITC
  • 17A2_Pure_CD3_Antibody_2_102418
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the section was stained with 10 µg/mL of Purified CD3 (clone 17A2) and Alexa Fluor® 488 anti-mouse/human CD45R/B220 antibody (green) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 594 anti-rat IgG2b (clone MRG2b-85) (red) for two hours at room temperature. The image was captured by 10X objective.
  • 17A2_Pure_CD3_Antibody_3_102418
    Fresh, frozen mouse spleen was stained with purified CD3 clone 17A2 conjugated and detected with a Cy3 CODEX™ oligonucleotide duplex (red). Samples were counterstained with B220 A647 (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
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100201 50 µg $30
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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
IP, ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration of 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Abdel-Gadir A, et al. 2019. Nat Med. 25:1164. PubMed
  2. Hamada T, et al. 2008. J Immunol. 180:1843. PubMed
  3. Pietras E, et al. 2016. Nat Cell Biol. 10.1038/ncb3346. PubMed
  4. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  5. Bernstein DL, et al. 2020. Brain Behav Immun. 87:543. PubMed
  6. Liu CJ, et al. 2020. Neuro Oncol. 22:1276. PubMed
  7. Doll JR, et al. 2020. PLoS Pathog. 16:e1008296. PubMed
  8. Li TT, et al. 2021. BMC Immunol. 22:55. PubMed
  9. Lu X, et al. 2021. J Exp Med. 218: . PubMed
  10. Cautivo KM, et al. 2022. Immunity. 55:254. PubMed
  11. Valet C, et al. 2022. J Clin Invest. 132: . PubMed
  12. Campisi L, et al. 2022. Nature. 606:945. PubMed
  13. Halin C, et al. 2022. EMBO J. 41:e111528. PubMed
  14. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  15. Zhao J, et al. 2022. J Clin Invest. 132: . PubMed
  16. Glaubitz J, et al. 2023. Gut. :. PubMed
  17. Fueyo-González F, et al. 2022. Immunity. 55:459. PubMed
  18. Potempa M, et al. 2022. J Immunol. 208:1742. PubMed
  19. Robles-Valero J, et al. 2022. Mol Oncol. 16:3533. PubMed
  20. Mammadli M, et al. 2023. Int J Mol Sci. 24:. PubMed
  21. Kang YA, et al. 2023. J Exp Med. 220:. PubMed
  22. Zhao J, et al. 2021. JCI Insight. 6:. PubMed
  23. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  24. Kulkarni NN, et al. 2020. J Invest Dermatol. 140:645. PubMed
  25. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  26. Xiao J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:386. PubMed
  27. Bergstrom K, et al. 2008. Infect Immun. 76:796. PubMed
  28. Mazor R, et al. 2018. Proc Natl Acad Sci U S A. 115:E733. PubMed
  29. Burrello C, et al. 2018. Front Med (Lausanne). 5:21. PubMed
  30. Hawse WF, et al. 2019. J Immunol. 203:2771. PubMed
  31. Xu L, et al. 2017. Int J Biol Macromol. . 10.1016/j.ijbiomac.2017.07.090. PubMed
  32. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  33. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  34. Zhang Z, et al. 2020. J Immunol. 204:3400. PubMed
  35. Haque M, et al. 2021. Viruses. 13:. PubMed
  36. Wabitsch S, et al. 2021. STAR Protoc. 2:100517. PubMed
  37. Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed
  38. Plumlee CR, et al. 2020. Cell Host Microbe. 29(1):68-82.e5. PubMed
  39. Rasoulouniriana D, et al. 2019. J Clin Invest. 129:4151. PubMed
  40. Thulasingam S, et al. 2019. Int J Mol Sci. 20. PubMed
  41. Kerdidani D, et al. 2019. Nat Commun. 10:1405. PubMed
  42. Hayashi Y, et al. 2017. J Cancer. 8:2478. PubMed
  43. Düsedau HP, et al. 2019. Glia. 67:193. PubMed
  44. Liu T, et al. 2020. Sci Adv. 6:eaay1497. PubMed
  45. Schafflick D, et al. 2020. Nat Commun. 247:11. PubMed
  46. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  47. Boothby IC, et al. 2021. Nature. 599:667. PubMed
  48. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  49. Mayer KA, et al. 2021. FASEB J. 35:e21217. PubMed
  50. Jiang P, et al. 2021. Nat Methods. 18:1181. PubMed
  51. Zhang Z, et al. 2021. Oncoimmunology. 10:1912472. PubMed
  52. Chen Y, et al. 2018. Oncoimmunology. 7:e1388484. PubMed
  53. Child KM et al. 2018. The Journal of Neuroscience. 38(31):6806-6824 . PubMed
  54. Recino A, et al. 2019. Gene Ther. 26:40. PubMed
  55. Adamson SE, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1020. PubMed
  56. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  57. Yoshihara T, et al. 2020. Gut Microbes. 11:1385. PubMed
  58. Yan J, et al. 2020. Cancer Immunol Res. 8:356. PubMed
  59. Chen L, et al. 2020. Front Immunol. 11:584458. PubMed
  60. He LD, et al. 2021. EMBO Rep. 22:e52196. PubMed
  61. Forsyth KS, et al. 2020. PLoS Pathog. 16:e1008685. PubMed
  62. Srey MT, et al. 2020. PLoS Negl Trop Dis. 14:e0008842. PubMed
  63. Karhadkar TR, et al. 2021. PLoS One. 16:e0245924. PubMed
  64. Fatkhullina AR et al. 2018. Immunity. 49(5):943-957 . PubMed
  65. Mainini F, et al. 2018. Nucleic Acid Ther. 28:225. PubMed
  66. Kopec A, et al. 2014. Toxicol Sci. 141:515. PubMed
  67. Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed
  68. Bittner-Eddy PD, et al. 2020. Front Immunol. 11:677. PubMed
  69. Dooley K, et al. 2021. Mol Ther. 29:1729. PubMed
  70. Zareie P, et al. 2017. J Neuroinflammation. 0.630555556. PubMed
  71. Wei S, et al. 2019. Virulence. 0.588888889. PubMed
  72. Miret JJ, et al. 2019. J Immunother Cancer. 7:32. PubMed
  73. Liu M, et al. 2018. Diabetologia. 61:2333. PubMed
  74. Davies CL, et al. 2019. Front Immunol. 10:1048. PubMed
  75. Shin J, et al. 2018. Diabetes. 67:1068. PubMed
  76. Schuler CF, et al. 2020. Allergy. 75:2279. PubMed
  77. Lim CS, et al. 2020. Front Immunol. 11:1186. PubMed
  78. You X, et al. 2022. Front Genet. 12:790990. PubMed
  79. Gilfillan CB, et al. 2020. Eur J Immunol. 50:505. PubMed
  80. Karhadkar TR, et al. 2020. bioRxiv. . PubMed
  81. Clark K, et al. 2019. Front Immunol. 10:2355. PubMed
  82. Sugita S, et al. 2017. Arthritis Res Ther. 19:176. PubMed
  83. Patterson AR, et al. 2019. J Allergy Clin Immunol. 143:245. PubMed
  84. Williams SK, et al. 2018. Sci Rep. 8:13628. PubMed
  85. Bartolacci C, et al. 2018. Cancer Immunol Res. 1.281944444. PubMed
  86. Fitzpatrick E, et al. 2017. PLoS One. 10.1371/journal.pone.0169273. PubMed
  87. Karhadkar TR, et al. 2020. Am J Physiol Lung Cell Mol Physiol. 318:L165. PubMed
  88. Levy BD, et al. 2020. J Allergy Clin Immunol. 145:335. PubMed
  89. Flietner E, et al. 2022. Sci Rep. 12:10616. PubMed
  90. Pan S, et al. 2021. Front Cell Dev Biol. 9:629306. PubMed
  91. Zelco A, et al. 2020. Front Cell Neurosci. 0.75625. PubMed
  92. Morgado P, et al. 2011. Infect Immun. 79:4401. PubMed
  93. Chen X, et al. 2019. Methods Mol Biol. 2048:131. PubMed
  94. Kawabe T, et al. 2017. Sci Immunol. 2:eaam9315. PubMed
  95. Moon H, et al. 2019. Nat Commun. 10:2225. PubMed
  96. Akiel M, et al. 2017. Cancer Res. 77:4014. PubMed
  97. von Roemeling CA, et al. 2020. Nat Commun. 11:1508. PubMed
  98. Fernández-Orth J, et al. 2020. Eur J Immunol. . PubMed
  99. Unsinger J, et al. 2021. J Leukoc Biol. 109:697. PubMed
  100. Brownlie D, et al. 2021. J Immunother Cancer. 9:. PubMed
  101. Liu J, et al. 2022. Stem Cell Res Ther. 13:247. PubMed
  102. Link CWM, et al. 2020. Front Immunol. 11:596772. PubMed
  103. Gaspar M, et al. 2020. Cancer Immunol Res. 0.875694444. PubMed
  104. Tilley S, et al. 2007. J Immunol . 178:3208. PubMed
  105. Kang LJ, et al. 2020. Sci Rep. 10:5603. PubMed
  106. Di Dalmazi G, et al. 2019. J Immunol. 202:1350. PubMed
  107. Lee YJ, et al. 2018. Front Microbiol. 9:83. PubMed
  108. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  109. Heib T, et al. 2021. Cell Reports. 35(6):109102. PubMed
  110. He Y, et al. 2021. eLife. 10:00. PubMed
  111. Englezou PC, et al. 2018. Mol Ther Nucleic Acids. 12:118. PubMed
  112. Katsuyama T, et al. 2019. J Clin Invest. 129:5411. PubMed
  113. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  114. Diao J, et al. 2018. J Immunol. 201:1306. PubMed
  115. Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed
  116. Zhang J, et al. 2019. Onco Targets Ther. 12:4985. PubMed
  117. Gonzales C, et al. 2016. PLoS One. 11:e0164557. PubMed
  118. Rastelli D, et al. 2021. J Clin Invest. :e150789. PubMed
  119. Moretti FA, et al. 2021. Sci Rep. 11:23221. PubMed
  120. Song M, et al. 2020. Nat Commun. 11:6298. PubMed
  121. Robles-Valero J, et al. 2021. EMBO J. 40:e108125. PubMed
  122. Kästele V, et al. 2021. Mucosal Immunol. 14:717. PubMed
  123. Lei F, et al. 2012. J Vis Exp. 63: 3986. PubMed
  124. Yadav A, et al. 2009. Am J Physiol Renal Physiol. 298:734. PubMed
  125. Shao Y, et al. 2017. Onco Targets Ther. 10:2675. PubMed
  126. Egaña–Gorroño L, et al. 2019. Atherosclerosis. 289:184. PubMed
  127. Regan–Komito D, et al. 2017. Front Immunol. 1.459027778. PubMed
  128. He X, et al. 2017. Cancer Biol Ther. 0.815277778. PubMed
RRID
AB_312658 (BioLegend Cat. No. 100201)
AB_312658 (BioLegend Cat. No. 100202)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-mouse CD3 17A2 FC
PE anti-mouse CD3 17A2 FC
Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Alexa Fluor® 647 anti-mouse CD3 17A2 FC,IHC-F,3D IHC,SB
Alexa Fluor® 488 anti-mouse CD3 17A2 FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse CD3 17A2 FC
Alexa Fluor® 700 anti-mouse CD3 17A2 FC
PerCP/Cyanine5.5 anti-mouse CD3 17A2 FC
PE/Cyanine7 anti-mouse CD3 17A2 FC
APC/Cyanine7 anti-mouse CD3 17A2 FC
Brilliant Violet 421™ anti-mouse CD3 17A2 FC,ICC
Brilliant Violet 570™ anti-mouse CD3 17A2 FC
Brilliant Violet 650™ anti-mouse CD3 17A2 FC
Brilliant Violet 785™ anti-mouse CD3 17A2 FC
Brilliant Violet 510™ anti-mouse CD3 17A2 FC
APC anti-mouse CD3 17A2 FC
Ultra-LEAF™ Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Brilliant Violet 605™ anti-mouse CD3 17A2 FC
Alexa Fluor® 594 anti-mouse CD3 17A2 IHC-F,FC,3D IHC,SB
Brilliant Violet 711™ anti-mouse CD3 17A2 FC
Biotin anti-mouse CD3 17A2 FC,IHC-F
PE/Dazzle™ 594 anti-mouse CD3 17A2 FC
APC/Fire™ 750 anti-mouse CD3 17A2 FC
Brilliant Violet 750™ anti-mouse CD3 17A2 FC
TotalSeq™-A0182 anti-mouse CD3 17A2 PG
TotalSeq™-B0182 anti-mouse CD3 17A2 PG
Spark Blue™ 550 anti-mouse CD3 17A2 FC
Spark NIR™ 685 anti-mouse CD3 17A2 FC
TotalSeq™-C0182 anti-mouse CD3 17A2 PG
APC/Fire™ 810 anti-mouse CD3 17A2 FC
PE/Fire™ 640 anti-mouse CD3 17A2 FC
Spark YG™ 570 anti-mouse CD3 17A2 IHC-F
PE/Fire™ 700 anti-mouse CD3 17A2 FC
PE/Cyanine5 anti-mouse CD3 17A2 FC
Spark Blue™ 574 anti-mouse CD3 Antibody 17A2 FC
Spark Violet™ 423 anti-mouse CD3 17A2 FC
PE/Fire™ 810 anti-mouse CD3 17A2 FC
Spark Red™ 718 anti-mouse CD3 17A2 FC
Spark UV™ 387 anti-mouse CD3 17A2 FC
Go To Top Version: 3    Revision Date: 01/04/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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