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PerCP/Cyanine5.5 anti-mouse CD103 Antibody

Pricing & Availability
Clone
2E7 (See other available formats)
Regulatory Status
RUO
Other Names
Integrin αIEL chain, Integrin αE chain, αE integrin, ITGAE
Isotype
Armenian Hamster IgG
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Product Citations
publications
2E7_PerCPCyanine55_CD103_Antibody_010319
C57BL/6 mouse splenocytes were stained with CD3 APC and CD103 (clone 2E7) PerCP/Cyanine5.5 (left) or Armenian hamster IgG PerCP/Cyanine5.5 isotype control (right).
  • 2E7_PerCPCyanine55_CD103_Antibody_010319
    C57BL/6 mouse splenocytes were stained with CD3 APC and CD103 (clone 2E7) PerCP/Cyanine5.5 (left) or Armenian hamster IgG PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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121415 25 µg $141
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121416 100 µg $347
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Description

CD103 is a type I transmembrane glycoprotein known as αE integrin or Integrin αIEL chain. It belongs to the integrin family and is primarily found on intestinal intraepithelial lymphocytes (IEL). CD103 is also expressed on a subpopulation of lamina propria T cells, epithelial dendritic cells, lamina propria-derived dendritic cells, and a small subset of peripheral lymphocytes. T regulatory cells express high level of CD103. The CD103 expression on lymphocytes can be induced upon activation and TGF-β stimulation. In association with integrin β7, CD103 is expressed as αE/β7 heterodimer. Mature CD103 protein can be cleaved into 2 chains, a 150 kD (C-terminal) chain and a 25 kD (N-terminal) chain, which remain linked by disulfide bonds. CD103 binds to E-cadherin and mediates homing of lymphocytes to the intestinal epithelium.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse intestinal intraepithelial lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining1,7 of acetone-fixed frozen sections, immunofluorescence2, and in vitro activation1.

Application References

(PubMed link indicates BioLegend citation)
  1. LeFrancois L, et. al, 1994. Eur. J. Immunol. 24:635. (FC, IHC, IP)
  2. Mysorekar IU, et. al, 2002. J. Biol. Chem. 277:37811. (FC, IF)
  3. Mikami N, et al. 2011. J. Immunol. 186:6886. PubMed
  4. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  5. Quinn KM, et al. 2013. J. Immunol. 191:5085. PubMed
  6. Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed
  7. Xiao B, et al. 2015. PLoS One 1:e0115333. (IHC)
Product Citations
  1. Sauter M, et al. 2022. STAR Protoc. 3:101664. PubMed
  2. Gao L, et al. 2023. J Nanobiotechnology. 21:56. PubMed
  3. Campisi L, et al. 2022. Nature. 606:945. PubMed
  4. Michalaki C, et al. 2022. Curr Protoc. 2:e505. PubMed
  5. Quinn K, et al. 2013. J Immunol. 191:5085. PubMed
  6. Ender F, et al. 2017. PLoS One. 12(2):e0172446. PubMed
  7. Ritzel R, et al. 2016. J Immunol. 196: 3318-3330. PubMed
  8. Sauter M, et al. 2022. iScience. 25:103677. PubMed
  9. Verhagen J, et al. 2013. PLoS One. 8:61334. PubMed
  10. Wang D, et al. 2018. Immunity. 48:659. PubMed
  11. Wong E, et al. 2019. Cell Rep. 29:3047. PubMed
  12. Khandelwal P, et al. 2013. PLoS One. 8:64193. PubMed
  13. Wiese A, et al. 2017. PLoS One. 10.1371/journal.pone.0184956. PubMed
  14. Zhang J, et al. 2022. Front Immunol. 13:931740. PubMed
  15. Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed
  16. Karsten C, et al. 2015. J Immunol. 194:1841. PubMed
  17. Blijswijk J, et al. 2015. J Immunol. 194:307. PubMed
  18. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  19. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  20. Cuburu N, et al. 2019. J Immunol. 202:1250. PubMed
  21. Pérez-Girón J, et al. 2015. J Vis Exp. 100: 52803. PubMed
  22. Port JR, et al. 2020. J Virol. 94:. PubMed
  23. Becker W, et al. 2021. J Crohns Colitis. 15:1032. PubMed
  24. Boucard-Jourdin M, et al. 2016. J Immunol. 197: 1968 - 1978. PubMed
  25. Kelly SH, et al. 2021. ACS Biomater Sci Eng. 7:1876. PubMed
  26. Huang S, et al. 2016. Immunol Cell Biol. 10.1038/icb.2016.75. PubMed
  27. Giampazolias E, et al. 2021. Cell. . PubMed
  28. Hoffmann FM, et al. 2018. Front Immunol. 9:1046. PubMed
  29. Bent EH, et al. 2021. Nat Commun. 12:6218. PubMed
  30. Penke LR, et al. 2020. Life Sci Alliance. 3:. PubMed
  31. Luck H, et al. 2019. Nat Commun. 10:3650. PubMed
  32. Yasuda T, et al. 2016. PLoS One. 11:e0167952. PubMed
  33. Da Mesquita S, et al. 2021. Science Advances. 7(21):. PubMed
  34. Meunier I, et al. 2015. PLoS One. 10: 0138055. PubMed
  35. Reed HO, et al. 2019. J Clin Invest. 130:2514. PubMed
  36. Pavelko K, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01532. PubMed
  37. Gaylo‐Moynihan A et al. 2019. Immunity. 51(2):298-309 . PubMed
  38. Kautz-Neu K, et al. 2011. J Exp Med. 208:885. PubMed
  39. Stotesbury C, et al. 2020. Aging Cell. 19:e13170. PubMed
  40. Ubags ND, et al. 2021. J Allergy Clin Immunol. 1049:147. PubMed
  41. Sheng J, et al. 2021. eLife. 10:00. PubMed
  42. Chen R, et al. 2021. Front Oncol. 11:743050. PubMed
  43. Spath S, et al. 2022. iScience. 25:104998. PubMed
  44. Lian M, et al. 2015. PLoS One. 10: 0132741. PubMed
  45. Waaler J, et al. 2020. Commun Biol. 3:196. PubMed
RRID
AB_1574957 (BioLegend Cat. No. 121415)
AB_1574957 (BioLegend Cat. No. 121416)

Antigen Details

Structure
Type I transmembrane glycoprotein, Integrin family, can be cleaved into 150 kD and 25 kD chains, associated with β7 integrin
Distribution

Majority of intestinal intraepithelial lymphocytes (IEL), subpopulation of lamina propria T cells, epithelial dendritic cells, small subset of peripheral lymphocytes, Treg cells.

Function
Retention and activation of CD103+ lymphocytes in the intestinal epithelium, regulate tissue-specific T cell homing.
Ligand/Receptor
E-Cadherin
Cell Type
Dendritic cells, Lymphocytes, T cells, Tregs
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Kilshaw PJ and SJ. Murant. 1990. Eur. J. Immunol. 20:2201.
2. Karecla PI, et al. 1995. Eur. J. Immunol. 25:852.
3. LeFrancois L, et al. 1994. Eur. J. Immunol. 24:635.
4. Sung SS, et al. 2006. J. Immunol. 176:2161.
5. Johansson-Lindbom B, et al. 2005. J. Exp. Med. 202:1063.
6. Dujardin HC, et al. 2004. Proc. Natl. Acad. Sci. USA. 101:14473.

Gene ID
16407 View all products for this Gene ID
UniProt
View information about CD103 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 01/03/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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