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Purified anti-Aquaporin-4 Antibody

Pricing & Availability
Clone
E5206 (See other available formats)
Regulatory Status
RUO
Other Names
MIWC, WCH4, AQP4
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
E5206_PURE_-Aquaporin-4_Antibody_120721
IHC staining of anti-Aquaporin-4 antibody (clone E5206) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5.0 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB) (Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 μm
  • E5206_PURE_-Aquaporin-4_Antibody_120721
    IHC staining of anti-Aquaporin-4 antibody (clone E5206) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5.0 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB) (Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 μm
  • E5206_PURE_-Aquaporin-4_Antibody_2_120721
    IHC staining of anti-Aquaporin-4 antibody (clone E5206) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5.0 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB) (Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 μm
  • E5206_PURE_-Aquaporin-4_Antibody_3_120721
    IHC staining of anti-Aquaporin-4 antibody (clone E5206) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5.0 µg/mL of purified anti-Aquaporin-4 antibody and Alexa Fluor® 488 anti-GFAP antibody (Cat. No. 837507) overnight at 4°C. Alexa Fluor® 594 Goat anti-mouse IgG antibody (Cat. No. 405326) was used for 2-hour incubation at room temperature, followed by nuclear counterstaining with DAPI (Cat. No. 422801). The slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 μm
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600351 25 µg $118
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600352 100 µg $293
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Description

Aquaporin-4 (AQP4) is a member of the water-selective channel family that is predominantly expressed in astrocytes in the central nervous system. AQP4 is a regulator of water homeostasis by exchanging water across the endfoot membranes in the brain. This protein is vital for maintaining the normal level of cerebrospinal fluid influx into the brain cortex, parenchyma, and perivascular space.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial recombinant human AQP4 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ICC, IHC-F, WB, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Ramadhanti J, et al. 2013. Monoclon Antib Immunogiagn Immunother. 32:270-276
RRID
AB_2904437 (BioLegend Cat. No. 600351)
AB_2904437 (BioLegend Cat. No. 600352)

Antigen Details

Structure
Aquaporin 4 is a 323 amino acid protein with a molecular mass of 35 kD.
Distribution

Tissue distribution: astrocytes in CNS 
Cellular distribution: plasma membrane, endosome, and cytoskeleton

Function
AQP4 facilitates the transport of water across the blood-brain interface.
Cell Type
Astrocytes
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Antigen References
  1. Salman MM, et al. 2021. Nat Rev Neurosci. 22:650-651
  2. Tang G and Yang GY. 2016. Int J Mol Sci. 17:1413
  3. Jarius S and Wildemann B. 2013. Brain Pathol. 23:661-83 
Gene ID
361 View all products for this Gene ID
UniProt
View information about Aquaporin-4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12/06/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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