Alexa Fluor® 594 anti-mouse IgD Antibody

Pricing & Availability
Clone
11-26c.2a (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin D
Isotype
Rat IgG2a, κ
A_11-26ca_A594_IgD_Antibody_040820
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS + rat/mouse/hamster serum for 30 minutes at room temperature. Then the section was stained with 5 µg/ml of Alexa Fluor® 594 anti-mouse IgD (Clone 11-26c-2a) (red) and 5 µg/ml Alexa Fluor® 647 anti-mouse CD3 (green) overnight at 4°C. The image was captured by 10X objective.
  • A_11-26ca_A594_IgD_Antibody_040820
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS + rat/mouse/hamster serum for 30 minutes at room temperature. Then the section was stained with 5 µg/ml of Alexa Fluor® 594 anti-mouse IgD (Clone 11-26c-2a) (red) and 5 µg/ml Alexa Fluor® 647 anti-mouse CD3 (green) overnight at 4°C. The image was captured by 10X objective.
  • B_11-26cdot2a_A594_IgD_Antibody_3D-IHC_092121.png
    Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-mouse CD3ε Antibody (clone 500A2) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse IgD Antibody (clone 11-26c.2a) Alexa Fluor® 594 at 5 µg/mL (yellow), and anti-mouse CD172a (SIRPα) Antibody (clone P84) Alexa Fluor® 647 at 5 µg/mL (magenta) and counterstained with DAPI (blue). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
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405740 100 µg $323.00
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Description

Surface IgD is an important B cell differentiation marker.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested
3D IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.0 - 10 μg/mL is recommended. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3, and spatial biology (IBEX)10,11.

Application References

(PubMed link indicates BioLegend citation)
  1. Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC)
  2. Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
  3. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
  4. Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
  5. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  6. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
  7. Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed
  8. Park SY, et al. 2013. J. Immunol. 190:1094. PubMed
  9. Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Whyte CE, et al. 2022. J Exp Med. 219:. PubMed
  2. Xu H, et al. 2020. Immunity. 51(4):696-708.e9.. PubMed
  3. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  4. Radice E, et al. 2020. Cell Reports. 32(5):107951. PubMed
RRID
AB_2565572 (BioLegend Cat. No. 405740)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation
Cell Type
B cells
Biology Area
Immunology
Gene ID
380797 View all products for this Gene ID
UniProt
View information about IgD on UniProt.org
Go To Top Version: 3    Revision Date: 09/21/2021

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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